4‐Methoxy‐α‐naphthol as a specific substrate for kinetic, zymographic and cytochemical studies on plant peroxidase activities

Ferrer, Marı́a A.; Calderón, Antonio A.; Muñoz, Romualdo; Barceló, A. Ros

doi:10.1002/pca.2800010203

Cited by 99 publications

(58 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The assay of peroxidase activity using 4-MN as a substrate was carried out as previously described (Ferrer et al 1990). The activity of peroxidase in oxidizing 4-HS to viniferin-type compounds was assayed as reported by Calder6n et al (1990a).…”

Section: Spectrophotometric Measurementsmentioning

confidence: 99%

Levels of 4-hydroxystilbene-oxidizing isoperoxidases related to constitutive disease resistance in in vitro-cultured grapevine

Calderón

Zapata

Pedreño³

et al. 1992

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

A zymographic screening of peroxidases (EC 1.11.1.7) capable of oxidizing 4-hydroxystilbene was carried out by means of the peroxidase-catalyzed oxidative coupling of 4-hydroxystilbene and 4-aminoantipyrine. This screening reveals that only a few isoperoxidases are active in oxidizing 4-hydroxystilbene to viniferin-type compounds in in vitro cultures of grapevine. Unlike total peroxidase activity measured with 4-methoxy-a-naphthol, the levels of total peroxidase activity measured using 4-hydroxystilbene are related to disease resistance against downy mildew in axillary bud cultures of Vitis vinifera and (Vitis vinifera x Vitis rupestris) x Vitis riparia. From this observation, and using the above zymographic assay, it was found that a 4-hydroxystilbene-oxidizing isoperoxidase was overexpressed in both leaves and stems of the hybrid in relation to the increase in disease resistance of this species. These results suggest that constitutive 4-hydroxystilbene-oxidizing isoperoxidases may participate through their role in viniferin synthesis in the constitutive resistance mechanism that grapevines show against downy mildew. Abbreviations

show abstract

Section: Spectrophotometric Measurementsmentioning

confidence: 99%

Levels of 4-hydroxystilbene-oxidizing isoperoxidases related to constitutive disease resistance in in vitro-cultured grapevine

Calderón

Zapata

Pedreño³

et al. 1992

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Isoelectric focusing and stain of peroxidase isoenzymes with 4-methoxy-α-naphthol was performed as described by Ferrer et al (1990). Controls were carried out in the absence of H 2 O 2 , and in the presence of 0·5 mol m -3 sodium p-chloromercuribenzoate, an inhibitor of ascorbate peroxidases (Chen & Asada 1989).…”

Section: Isoelectric Focusing and Stain Of Peroxidase Isoenzymesmentioning

confidence: 99%

Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans

Ferrer

Barceló

1999

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

Sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP) are two nitric oxide (NO)-releasing compounds that, when used at 5·0 mol m -3 concentrations, are capable of releasing NO in the aqueous phase at a rate of 35 ± 4 and 47 ± 5 µmol m -3 s -1 , respectively. For this reason, the effect of SNP and SNAP on coniferyl alcohol peroxidase and on H 2 O 2 production by the lignifying xylem of Zinnia elegans (L.) has been studied in order to ascertain whether NO, which is a synchronizing chemical messenger in animals and an air pollutant, has any effect on these plant-specific metabolic aspects. The results showed that both SNP and SNAP provoke an inhibition in the mol m -3 concentration range of the coniferyl alcohol peroxidase activity of a basic peroxidase isoenzyme present in the intercellular washing fluid of Z. elegans. The effect of these NO-releasing compounds on peroxidase was confirmed through histochemical studies, which showed that xylem peroxidase was totally inhibited by treatment with these NO donors at 5·0 mol m -3 , and by NO at a concentration change rate of 55 ± 5 and 110 ± 9 µmol m -3 s -1 . However, SNP, at 5·0 mol m -3 , does not have any effect on H 2 O 2 production by the xylem of Z. elegans. The fact that SNP and SNAP are two structurally dissimilar compounds which only share the common ability to release NO in aqueous buffer, and that similar results were obtained when using NO itself, suggest that NO could be considered as an inhibitor of coniferyl alcohol peroxidase which does not affect H 2 O 2 production in the xylem of Z. elegans.Key-words: Zinnia elegans; coniferyl alcohol oxidation; H 2 O 2 production; inhibition; nitric oxide; peroxidase; xylem.Abbreviations: IWF, intercellular washing fluid; pCMB, pchloromercuribenzoic acid; SNAP, S-nitroso-N-acetyl-penicillamine; SNP, sodium nitroprusside; TMB, 3,3',5,5'-tetramethylbenzidine. INTRODUCTIONNitric oxide (NO) is a paramagnetic relatively stable freeradical molecule involved in many physiological processes in animals, where it serves as a synchronizing chemical messenger involved in vasorelaxation, platelet inhibition, neurotransmission, cytotoxicity and immunoregulation (Anbar 1995). In animal cells, NO is synthesized by the enzyme NO synthase (Knowles 1997), and most of its biological regulatory properties have been explained on the basis of its capacity to act as an iron ligand in haemoproteins (Stamler, Singel & Loscalzo 1992). Dual (activating or inhibitory) effects of NO on haemoproteins have been described (Tsai 1994), and the nature of the effect has been suggested to depend to a great extent on the resting state (oxidation state) of the haemoprotein, which conditions the ligand properties and the electronic configuration of the haem iron (Tsai 1994). In the case of catalase, it has been shown that NO reversibly binds to the haem of catalase and inhibits H 2 O 2 breakdown with a K i of about 0·2 mmol m -3 (Brown et al. 1997).Evidence is emerging to support the possibility that NO may also act as chemical mess...

show abstract

“…Peroxidase activity (POD EC 1.11.1.7) was measured following the oxidation of 3,3 0 ,5,5 0 -Tetramethylbenzidine (TMB) at 652 nm (e = 26,9 mM -1 cm -1 ), according to Ferrer et al (1990).…”

Section: Enzyme Assaysmentioning

confidence: 99%

Bioremediation of dry olive-mill residue removes inhibition of growth induced by this waste in tomato plants

García‐Sánchez

Paradiso

García-Romera

et al. 2013

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

The disposal of dry olive-mill residue, the waste product from olive oil production, is a serious environmental issue. Dry olive-mill residue, being rich in organic and inorganic nutrients, could be used as fertilizer; however, it contains phenolic compounds that can inhibit plant growth. In order to clarify whether bioremediation of this waste could be a valuable strategy for its reuse, the effect of aqueous extract of dry olive-mill residue, untreated or bioremediated by the saprobe fungi Coriolopsis rigida and Penicillium chrysogenum-10, has been analyzed in relation to some physiological parameters of tomato plants. The data show that aqueous dry olive-mill residue significantly reduces the biomass of roots and shoots. In particular, it causes a dramatic reduction in root length, area, and volume as well as in the number of root tips. At an early stage, aqueous dry olive-mill residue also reduces the content of chlorophyll a and b and the efficiency of PS II. The inhibition of growth seems to be due to the increase in phenolic compounds that induce oxidative stress. Interestingly, when plants are treated with aqueous dry olive-mill residue bioremediated by saprobe fungi a decrease in phenolic content and an alleviation of oxidative stress occur. In conclusion, the results show that bioremediation of aqueous dry olive-mill residue is a useful tool to remove most of the inhibiting effects of this waste on plant growth.

show abstract

4‐Methoxy‐α‐naphthol as a specific substrate for kinetic, zymographic and cytochemical studies on plant peroxidase activities

Cited by 99 publications

References 25 publications

Levels of 4-hydroxystilbene-oxidizing isoperoxidases related to constitutive disease resistance in in vitro-cultured grapevine

Levels of 4-hydroxystilbene-oxidizing isoperoxidases related to constitutive disease resistance in in vitro-cultured grapevine

Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans

Bioremediation of dry olive-mill residue removes inhibition of growth induced by this waste in tomato plants

Contact Info

Product

Resources

About

4‐Methoxy‐α‐naphthol as a specific substrate for kinetic, zymographic and cytochemical studies on plant peroxidase activities

Cited by 99 publications

References 25 publications

Levels of 4-hydroxystilbene-oxidizing isoperoxidases related to constitutive disease resistance in in vitro-cultured grapevine

Levels of 4-hydroxystilbene-oxidizing isoperoxidases related to constitutive disease resistance in in vitro-cultured grapevine

Differential effects of nitric oxide on peroxidase and H2O2 production by the xylem of Zinnia elegans

Bioremediation of dry olive-mill residue removes inhibition of growth induced by this waste in tomato plants

Contact Info

Product

Resources

About

Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans