4. Comparison of various methods of disinfecting irreversible hydrocolloid impressions using chlorhexidine gluconate: Assessment of antimicrobial efficacy & dimensional changes

Abstract

The aim of the study was to investigate different procedures to check for the most effective disinfection method and most dimensionally accurate impressions post-treatment with disinfectant. Five alginate impressions, each of thirty-two dentate patients were made with 24 hours interval. Impressions were divided into five categories: Group I included alginate impression with only water rinsing (Control). Group II were with immersion in chlorhexidine gluconate solution, Group III were spraying and Group IV were impression of alginate mixed with chlorhexidine solution. Group V comprised of impressions taken after pre-procedural mouth rinsing. Samples were inoculated in blood agar media for 3 days at 37°C. The impressions were poured in type III dental stone. The inter-molar distance, inter-canine distance, the antero-posterior distance and the depth of the cast were measured with Digital Vernier calipers. The results indicate the presence of highest number of bacterial colonies on Group I impression i.e. the control group. Least bacterial count was found in Group V followed by Group III, Group II and Group IV. Highest dimensional changes were seen in Group IV followed by Group III and Group II casts. Group V was found to be most dimensionally stable. Within the limitations of the study, it was concluded that the most effective and accurate method of disinfection is pre-procedural rinsing followed by spraying and immersion. Internal disinfection was found to be the least effective method with high bacterial count as well as high dimensional changes.

Recomendaciones para prevención y control de infecciones por SARS-CoV-2 en odontología

“…Las impresiones dentales, registros de mordida, prótesis en prueba, modelos yeso, pueden llevar a transmitir enfermedades, ya que estuvieron en contacto con fluidos corporales. Para reducir la posibilidad de infección cruzada entre pacientes, dentistas, asistentes y técnicos de laboratorio, es esencial que todas las impresiones y todo trabajo de prostodoncia que estuvo en contacto con la boca del paciente se desinfecten 41 .…”

“…Gupta y cols. 41 , realizaron un estudio comparativo entre varios métodos de desinfección de impresiones y concluyeron que al lavar la impresión con agua no se logra disminuir la carga microbiana de la impresión, el método de desinfección más efectivo y preciso es el enjuague bucal previo al procedimiento, que además no causa impressions and other prosthodontics materials such as bite records are to be removed from the patient's mouth, they must be completely disinfected 28 . Due to its antiviral effectiveness, chlorine solutions are recommended 36 .…”

“…Dental impressions, bite records, trial prostheses, plaster models, can lead to transmit diseases, since they were in contact with body fluids. To reduce the possibility of cross infection among patients, dentists, assistants, and laboratory technicians, it is essential that all impressions and any prosthodontics work that came in contact with the patient's mouth be disinfected 41 .…”

“…The American Dental Academy (ADA) and the International Dental Federation (IDF) insist on disinfecting all impressions taken from patients before sending them to laboratories. Gupta et al 41 , carried out a comparative study between various methods of disinfection of impressions and concluded that washing the impression with water does not reduce the microbial load of the impression, the most effective and accurate disinfection method is pre-procedure mouthwash, which also did not cause • La saliva, sangre y la carga biológica deben limpiarse primero de la impresión antes de la desinfección.…”

“…• Los materiales de impresión primero se lavan en agua, luego se desinfectan teniendo en cuenta que los materiales utilizados tienen una tolerancia limitada a la inmersión en líquidos, por lo tanto, esto debe minimizarse y después de desinfectar finalmente se vuelve a enjuagarse con agua corriente antes de seguir procesándose o enviarse al laboratorio 41 .…”