4-Amino-6-hydroxy-2-mercaptopyrimidine capped gold nanoparticles as fluorophore for the ultrasensitive and selective determination of l-cysteine

Shankar, Sekar; John, S. Abraham

doi:10.1016/j.snb.2015.07.092

Cited by 36 publications

(10 citation statements)

References 45 publications

(47 reference statements)

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“…Since the degree of attenuation on the FRET efficiency is strongly dependent on the amount of L-Cys, a highly sensitive assay for L-Cys detection was developed. Compared to some other complicated and costly biological methods for constructing an AuNP complex system [18,19,21], the FRET strategy here, based on electrostatic interaction, is simple and practical. The principle of this method, based on the decrease of the PL intensity of the SiNPs-ethanol solution, results in FRET assemblies between amino-capped SiNPs and citrate-stabilized AuNPs via electrostatic interaction.…”

Section: Resultsmentioning

confidence: 99%

“…So the effect of the pH range on the interaction of L-Cys with AuNPs was also studied. It has already been reported that only at relatively low pH values does the amine group of amino acids bind with AuNPs, whereas the thiol group can bind with AuNPs at high pH values (pH > 5) [18]. In this experiment, the pH of the SiNPs-ethanol and AuNPs solution was found to be 5.6 and the other pH values of the solutions were adjusted to 7.0, 8.0, 9.0 and 10.0 with 1.0 M NaOH.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, the construction of fluorescent materials for L-Cys sensing is designed to be “off-on” [10,14,15,16,17]. However, the fluorescence spectrum band of traditional fluorescent materials including rhodamine B, gold nanoclusters (AuNCs) or silica nanoparticles is relatively broad and asymmetric [18,19,20], and the effective overlap of the absorbance spectrum of AuNPs or the fluorescence spectrum band of traditional fluorophores is narrow, which affects the detection sensitivity of the immunosensor. There is a wide overlap of the absorbance spectrum of AuNPs and the fluorescence emission wavelength of fluorescent quantum dots (QDs), but the method is limited by a need for well-trained professionals and the high cost of QDs.…”

Section: Introductionmentioning

confidence: 99%

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Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution

Zhang

Jia

2017

Sensors

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A new type of fluorescence “off-on” probe was designed for L-Cysteine (L-Cys) based on the fluorescence resonance energy transfer (FRET) between negatively charged amino-capped porous silicon nanoparticles (SiNPs) and positively charged citrate-stabilized Au nanoparticles (AuNPs). In this proposed FRET immunosensor, novel water-soluble amino-conjugated porous SiNPs in ethanol with excellent photoluminescence properties act as the energy donor. Excellent quenching efficiency between SiNPs-ethanol and citrate-stabilized AuNPs by electrostatic interaction via FRET provides an ideal “off-state” (turn-off). The addition of L-Cys leads to releasing the adsorbed AuNPs from the surface of SiNPs and hence the fluorescence emission of SiNPs-ethanol is restored (turn-on), which means the coordination ability of the thiols with AuNPs is stronger than that of the electrostatic interaction. The fluorescence intensity of SiNPs-AuNPs in ethanol is sensitive to L-Cys, and such a restored fluorescence is proportional to the concentration of L-Cys. The method will broadly benefit the development of a new thiol biosensor based on nanostructured porous materials, and the proposed procedure is also expected to develop a variety of functional nanoparticles to form other novel kinds of nanosensors.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution

Zhang

Jia

2017

Sensors

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“…Therefore, it is necessary to develop a technique for cysteine detection. L‐cysteine detection has been reported using fluorimetry , chemiluminescence , high‐performance liquid chromatography , and electrochemistry . Among these, electrochemical detection has received considerable attention owing to the high sensitivity, speed, and low cost .…”

Section: Introductionmentioning

confidence: 99%

Photoelectrochemical Detection of L‐Cysteine with a Covalently Grafted ZnTAPc‐Gr‐based Probe

et al. 2020

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L‐cysteine plays a vital role in organisms, and is an important biomarker for many pathological diseases that seriously affect human health. In the study, a photoelectrochemical (PEC) probe with zinc‐tetramine phthalocyanine covalently grafted to graphene oxide (ZnTAPc‐Gr) was developed for L‐cysteine detection. Graphene oxide (GO) with carboxyl was used to immobilize zinc‐tetramine phthalocyanine (ZnTAPc) with amidogen (a graft structure formed by an amide covalent bond), which could firmly immobilize ZnTAPc, thereby improving the photoelectrochemical performance and stability. L‐cysteine molecule, an electron acceptor, was specifically recognized by the PEC probe, exhibiting a decrease in the photocurrent signal. Under the optimal experimental conditions, the fabricated PEC probe exhibited excellent performance in L‐cysteine analysis within a linear range of 0.25–113 μM and a detection limit of 11.4 nM. The PEC probe showed high sensitivity, selectivity, and stability. The method described herein provides an effective strategy for PEC probe construction for L‐cysteine detection, and can also serve as a promising PEC platform for the analyses of other small molecules.

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“…4-Amino-6-hydroxy-2-mercaptopyrimidine monohydrate (AHMP) is an interesting ligand. It contains a thiol group which can self-assemble on the AuNP surface [29,30]. Additionally, the amine and hydroxyl groups available in AHMP are free from binding.…”

Section: Introductionmentioning

confidence: 99%

A Transparency Sheet-Based Colorimetric Device for Simple Determination of Calcium Ions Using Induced Aggregation of Modified Gold Nanoparticles

Duenchay

Chailapakul

Siangproh

2019

IJMS

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A simple and novel transparency sheet-based colorimetric detection device using gold nanoparticles (AuNPs) modified by 4-Amino-6-hydroxy-2-mercaptopyrimidine monohydrate (AHMP) was fabricated and developed for the determination of calcium ions (Ca2+). The detection was based on a colorimetric reaction as a result of the aggregation of modified AuNPs induced by Ca2+ due to the ability to form strong electrostatic interactions between positively charged Ca2+ and negatively charged modified AuNPs. Probe solution changes color from red to blue in the presence of Ca2+ and can be observed by the naked eyes. To verify the complete self-assembly of the AHMP onto the AuNP surface, the modified AuNPs were characterized using ultraviolet–visible spectroscopy and zeta potential measurements. Under optimal conditions, a quantitative linearity was 10 to 100 ppm (R2 = 0.9877) with a detection limit of 3.05 ppm. The results obtained by the developed method were in good agreement with standard atomic absorption spectrometry (AAS) results and demonstrated that this method could reliably measure Ca2+. Overall, this novel alternative approach presents a low-cost, simple, sensitive, rapid, and promising device for the detection of Ca2+.

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4-Amino-6-hydroxy-2-mercaptopyrimidine capped gold nanoparticles as fluorophore for the ultrasensitive and selective determination of l-cysteine

Cited by 36 publications

References 45 publications

Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution

Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution

Photoelectrochemical Detection of L‐Cysteine with a Covalently Grafted ZnTAPc‐Gr‐based Probe

A Transparency Sheet-Based Colorimetric Device for Simple Determination of Calcium Ions Using Induced Aggregation of Modified Gold Nanoparticles

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