Abstract:A simple, precise and stability-indicating HPLC method was developed and validated for the simultaneous determination of anti-diabetic drugs. The separation was achieved on ACE 3 150mm*4.6mm, 3.5µm column with gradient flow. The mobile phase at a flow rate of 1.5 mL min −1 consisted of 10mM sodium hexane sulphonate monohydrate and 10mM Potassium dihydrogen phosphate buffer with acetonitrile and methanol in gradient ratio. The UV detection was carried out at 210 nm. The method was successfully validated in acco… Show more
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