3P124 Proton pathway and rotary motion of c-ring in F_o analyzed by all-atom and coarse-grained molecular dynamics simulation.(Membrane proteins,The 48th Annual Meeting of the Biophysical Society of Japan)

Abstract

revioubly we studied Loi relatien bctween the mcltallg of2D crystaltine array of bR and the photobleachmg The rcsults suggested thelt the wcakenmg of mLermelecular interaLuen is responsible for llTeversible photobleachmg In ths work we carried out the measurement of vistble Lircu]ar dichioism (CD) spectTaandthephotobleachmgexpeninenLofbRrecenstitutedintothreekinds of phosphatid}ILhehne (PC) vcsicles vvith dlfierent acyl cham lengtb Vlsible CD tspectra showLd an exciton to potsitsve transLLon of bR rLLensututed into dimyns{oyl dipellmito}1 and distearoyl PC iesicles around 17 35 and 50 OC respeLtiiely Thebe trelnsmon tLmperatures were close to the main tran smon of the reeonstatuted veslcles meagured by LaloTimeL"L measurcment Irrevergible photob]eaching was stdrted to occur at the same tempciatare re gions as the Lhdnge in the vistble CD trelnsmon Llar]fytng the Lorielation between the mclting of crystalhne dsgembly and the photobledching et bR On thc other hdnd lhc thermdl bteachmg m the dark wels not obberved below 60 "C for eaLh sample The plaubible model for irreierslble photobleachmg is discussed based on these expenrncnta} results 3P122 lsuEomusclente) 2za-"-emh\y:-v-s,3yccs6gmwFvaFvyx nt'9-AcrBOneut All atom molecujar dy"amicg gimu]ation of multidrug eMuN transporterALrB Yhmane Vmsunell JkLgllch/ (fokohana Cttv Un iettir}