3P-0657 Xenomyoblasts transplantation into porcine myocardium with transient immunosuppression

Haider, Kh. H; Lei, Yuk Man; Shujia, J.; Tang, Ming; Chua, Tju Siang; Law, Peter K.; Sim, Eugene K.W.

doi:10.1016/s1567-5688(03)90877-4

Cited by 2 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even a small contamination of fibroblasts in the separated myoblasts could result in a continuous reduction of purity during cell expansion, as fibroblasts grow faster than myoblasts in culture using plain surface. For clinical application, it was previously estimated that at least one billion pure myoblasts are required for efficient treatment [3]. Andreina et al [23] previously reported that irrespective of patient age, an adequate number of cells can be produced within one month; however, only half of the population was identified as myoblasts.…”

Section: Isolation Of Cells From Skeletal Muscle Produces Mixed Populmentioning

confidence: 99%

“…Over the past decades, myoblasts have gained remarkable attention for their clinical application due to their easy accessibility and availability, in vitro self-renewal capacity, stress resistance and lack of tumorigenicity [2,3].…”

Section: Introductionmentioning

confidence: 99%

“…Myoblasts have been tested for their application as a cell therapy to regenerate muscles [2][3][4][5][6][7][8][9] and as a gene delivery system [10] to treat muscle and non-muscle diseases. Several preclinical and clinical studies have shown the functional benefits of myoblast transplantation in the treatment of congenital muscle disorder [4,10], cardiac diseases [2,3,5,6], urinary incontinence [7] and traumatic muscle damage [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Several preclinical and clinical studies have shown the functional benefits of myoblast transplantation in the treatment of congenital muscle disorder [4,10], cardiac diseases [2,3,5,6], urinary incontinence [7] and traumatic muscle damage [8,9].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

One-Step Purification of Human Skeletal Muscle Myoblasts and Subsequent Expansion Using Laminin-Coated Surface

Chowdhury

Ismail

Chee

et al. 2015

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

Skeletal myoblasts have been extensively used to study muscle growth and differentiation, and were recently tested for their application as cell therapy and as a gene delivery system to treat muscle and nonmuscle diseases. However, contamination of fibroblasts in isolated cells from skeletal muscle is one of the long-standing problems for routine expansion. This study aimed to establish a simple one-step process to purify myoblasts and maintain their purity during expansion. Mixed cells were preplated serially on laminin- and collagen type I-coated surfaces in a different array for 5, 10, and 15 min. Immunocytochemical staining with antibodies specific to myoblasts was performed to evaluate myoblast attachment efficiency, purity, and yield. It was found that laminin-coated surface favors the attachment of myoblasts. Highest myoblast purity of 78.9% ± 6.8% was achieved by 5 min of preplating only on the laminin-coated surface with a yield of 56.9% ± 3.3%. Primary cells, isolated from skeletal muscle (n = 4), confirm the enhancement of purity through preplating on laminin-coated surface for 5 min. Subsequent expansion after preplating enhanced myoblast purity due to an increase in myoblast growth than fibroblasts. Myoblast purity of ∼ 98% was achieved when another preplating was performed during passaging. In conclusion, myoblasts can be purified and efficiently expanded in one step by preplating on laminin-coated surface, which is a simple and robust technique.

show abstract

Section: Isolation Of Cells From Skeletal Muscle Produces Mixed Populmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%