3M™ Molecular Detection system versus MALDI-TOF mass spectrometry and molecular techniques for the identification of Escherichia coli 0157:H7, Salmonella spp. &amp; Listeria spp.

Loff, Marché; Maré, L.; Kwaadsteniet, Michéle de; Khan, Wesaal

doi:10.1016/j.mimet.2014.03.015

Cited by 17 publications

(9 citation statements)

References 41 publications

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“…This effect was even more apparent in cattle feed where only one repeat at the 10 0 CFU/25 g level was positive by LAMP-BART and conventional LAMP and none by 3M MDA Salmonella . Another recent study using 3M MDA Salmonella in water sources also showed it to be less effective than PCR in detecting Salmonella [ 38 ]. It is hypothesized that natural flora present in cattle feed or compounds released during processing may have affected Salmonella survival and growth during enrichment, causing the low sensitivity in detection.…”

Section: Discussionmentioning

confidence: 99%

Rapid detection of Salmonella in food and feed by coupling loop-mediated isothermal amplification with bioluminescent assay in real-time

et al. 2016

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BackgroundSalmonella is among the most significant pathogens causing food and feed safety concerns. This study examined the rapid detection of Salmonella in various types of food and feed samples by coupling loop-mediated isothermal amplification (LAMP) with a novel reporter, bioluminescent assay in real-time (BART). Performance of the LAMP-BART assay was compared to a conventional LAMP and the commercially available 3M Molecular Detection Assay (MDA) Salmonella.ResultsThe LAMP-BART assay was 100 % specific among 178 strains (151 Salmonella and 27 non-Salmonella) tested. The detection limits were 36 cells per reaction in pure culture and 104 to 106 CFU per 25 g in spiked food and feed samples without enrichment, which were comparable to those of the conventional LAMP and 3M MDA Salmonella but 5–10 min faster. Ground turkey showed a strong inhibition on 3M MDA Salmonella, requiring at least 108 CFU per 25 g for detection. The correlation between Salmonella cell numbers and LAMP-BART signals was high (R2 = 0.941–0.962), suggesting good quantification capability. After 24 h enrichment, all three assays accurately detected 1 to 3 CFU per 25 g of Salmonella among five types of food (cantaloupe, ground beef, ground turkey, shell eggs, and tomato) and three types of feed (cattle feed, chicken feed, and dry dog food) examined. However, 101 CFU per 25 g was required for cattle feed when tested by 3M MDA Salmonella.ConclusionsThe Salmonella LAMP-BART assay was rapid, specific, sensitive, quantitative, and robust. Upon further validation, it may become a valuable tool for routine screening of Salmonella in various types of food and feed samples.

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Section: Discussionmentioning

confidence: 99%

Rapid detection of Salmonella in food and feed by coupling loop-mediated isothermal amplification with bioluminescent assay in real-time

et al. 2016

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“…Since Biotyper‐ or VITEK‐MS‐based spectra pattern comparison does not involve any protease treatment, the exact composition of the spectra is unknown. However, the spectra are often very unique for most bacteria, especially at genus level . Statistical methods such as phyloproteomic principal component analysis can be used to find the patterns and unique peaks of individual strains , and software such as Samaris can be used for strain identification by comparing the calibrated mass spectra to the reference spectra .…”

Section: Common Ms Platforms In Bacteria Identification and Typing (Fmentioning

confidence: 99%

Recent development of mass spectrometry and proteomics applications in identification and typing of bacteria

Cheng

Chui

Domish

et al. 2016

Proteomics Clinical Apps

101

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Identification and typing of bacteria occupy a large fraction of time and work in clinical microbiology laboratories. With the certification of some MS platforms in recent years, more applications and tests of MS‐based diagnosis methods for bacteria identification and typing have been created, not only on well‐accepted MALDI‐TOF‐MS‐based fingerprint matches, but also on solving the insufficiencies of MALDI‐TOF‐MS‐based platforms and advancing the technology to areas such as targeted MS identification and typing of bacteria, bacterial toxin identification, antibiotics susceptibility/resistance tests, and MS‐based diagnostic method development on unique bacteria such as Clostridium and Mycobacteria. This review summarizes the recent development in MS platforms and applications in bacteria identification and typing of common pathogenic bacteria.

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“…There are reports where intact protein biomarkers have been defined by proteogenomics in order to differentiate microorganisms and discriminate pathogens of a given genus or clade [51][52][53] . However, the single MALDI-MS approach suffers several limitations due to lack of peptide sequence information, need of pure cultures to produce consistent results, and effect of culture conditions on spectral quality and ensuing identifications [23][24][25] . The inability to differentiate high-consequence pathogens from related bacterial species can be a serious issue for clinical diagnostic laboratories and more so in a biothreat perspective 28,50,54 .…”

Section: Discussionmentioning

confidence: 99%

“…MS platforms such as Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS) or liquid chromatography tandem mass spectrometry (LC-MS/MS) are gaining popularity in microbiological research, largely for diagnostic applications, using mass fingerprinting or peptide sequencing approaches 18 .MALDI-TOF MS is emerging as a rapid, robust, and cost-effective technology for a routine bacterial identification method, largely in clinical diagnostic laboratories [19][20][21][22] . For example, MALDI Biotyper and VITEK MS have been approved by the U.S. Food and Drug Administration for bacterial identification in clinical diagnostic laboratories [23][24][25][26] . Both these MALDI-TOF-MS-based platforms ionize intact proteins extracted from whole cell culture without specific protease treatment 19,27 .…”

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confidence: 99%

Elucidation of protein biomarkers for verification of selected biological warfare agents using tandem mass spectrometry

Rajoria

Sabna

Babele

et al. 2020

Sci Rep

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Some pathogens and toxins have the potential to be used as weapons of mass destruction and instigate population-based fear. Efforts to mitigate biothreat require development of efficient countermeasures which in turn relies on fast and accurate methods to detect the biological agents in a range of complex matrices including environmental and clinical samples. We report here an mass spectrometry (MS) based methodology, employing both targeted and shot-gun approaches for the verification of biological agents from the environmental samples. our shot-gun methodology relied on tandem MS analysis of abundant peptides from the spiked samples, whereas, the targeted method was based on an extensive elucidation of marker proteins and unique peptides resulting in the generation of an inclusion list of masses reflecting relevant peptides for the unambiguous identification of nine bacterial species [listed as priority agents of bioterrorism by centre for Disease control and prevention (cDc)] belonging to phylogenetically diverse genera. the marker peptides were elucidated by extensive literature mining, in silico analysis, and tandem MS (MS/MS) analysis of abundant proteins of the cultivated bacterial species in our laboratory. A combination of shot-gun MS/MS analysis and the targeted search using a panel of unique peptides is likely to provide unambiguous verification of biological agents at subspecies level, even with limited fractionation of crude protein extracts from environmental samples. the comprehensive list of peptides reflected in the inclusion list, makes a valuable resource for the multiplex analysis of select biothreat agents and further development of targeted MS/MS assays.The realization that that some pathogenic microbes and toxins of biological origin can be used as weapons of mass destruction has gained prominence especially in the backdrop of growing concerns for bioterrorism. Biological select agents are derived from biological sources, are capable of causing significant damage to human health and safety, and can be overtly or covertly used for ideological, political, or financial gain 1 . U.S. Department of Health and Human Services (HHS) and the U.S. Department of Agriculture (USDA) have prioritized these select agents 2 and CDC has classified the agents of bioterrorism under the categories A through C 3 . It includes several toxins, bacteria, and viruses that are prioritized based on their high morbidity and mortality, dose of infections, environmental stability, production ease, and lack of an established countermeasure. Our vulnerability to biological warfare agents was realized after the anthrax letter attack of 2001 4 ; the mortality rate of inhalational anthrax is 100% when left untreated. The high-consequence bacterial biowarfare (BW) agents encompass wide phylogenetic lineages including the causative agents of plague (Yersinia pestis), anthrax (Bacillus anthracis), brucellosis (Brucella spp), and tularaemia (Francisella tularensis). Missile warheads, manned or unmanned spray-tanks coupled to the...

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3M™ Molecular Detection system versus MALDI-TOF mass spectrometry and molecular techniques for the identification of Escherichia coli 0157:H7, Salmonella spp. & Listeria spp.

Cited by 17 publications

References 41 publications

Rapid detection of Salmonella in food and feed by coupling loop-mediated isothermal amplification with bioluminescent assay in real-time

Rapid detection of Salmonella in food and feed by coupling loop-mediated isothermal amplification with bioluminescent assay in real-time

Recent development of mass spectrometry and proteomics applications in identification and typing of bacteria

Elucidation of protein biomarkers for verification of selected biological warfare agents using tandem mass spectrometry

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