3H-spiroperidol binding to human peripheral mononuclear cells: Methodological aspects

Abstract

3H-spiroperidol binding to lymphocytes has been proposed as

3H-spiroperidol binding to peripheral mononuclear cells in schizophrenic and healthy subjects

“…We could previously demonstrate that the complex model of one specific and one nonspecific binding site is the most adequate to describe this atypically shaped "saturation curve" (Wodarz et al 1992). Therefore, data are presented only for this model.…”

“…There were no statistically significant differences in the characteristic binding parameters between the different subtypes of schizophrenic disorder or to the controls. Binding parameters Ka and B,~x were calculated by using the model of one s~cific an_d one nonspecific binding site (Le Fur et al 1983;Bidart et al 1983;Wodarz et al 1992). Fi~m'c 2.…”

“…Binding para_meters Kd and Bmax were ,-~l,-,,l~tod according to the m~-~de! of one specific and one nonspecific binding site (Le Fur et al 1983;Bidart et al 1983;Wodarz et al 1992).…”

“…As we previously have described in detail (Wodarz et al 1992), some well-known but obviously uncontrolled methodological issues might have contributed to this conIxoversial results (e.g., unspecific filter binding, which increases in the presence of ( + )-butaclamol as a displacer, or a variable amount of contaminating granulocytes, which do not exhibit the spiroperidol binding site). Moreover, selection criteria of schizophrenic patients differed between the groups.…”

“…In previous reports we described in detail that some well-known, but obviously uncontrolled pitfails in binding assays using viable cells might have contributed to this controversy. Moreover, because of an atypically shaped saturation curve, different mathematical methods to analyze the data were used throughout the literature (Wodarz et al 1989(Wodarz et al , 1992. The potential clinical importance of 3H-spiroperidol binding to lymphocytes as a tool to diagnose schizophrenia, to identify individuals at risk (Bondy and Ackenheil 1987) and/or to predict the response to neuroleptic treatment (Grodzicki et al 1990) led us to reinvestigate 28 patients fulfilling DSM-lll-R-criteria of schizophrenia with our previously reported methodology.…”