[3H]Ro 15-1788 binding sites to brain membrane of the saltwater Mugil cephalus

Betti, Laura; Giannaccini, Gino; Gori, Michele; Bistocchi, M; Lucacchini, Antonio

doi:10.1016/s1532-0456(00)00195-2

Cited by 7 publications

(6 citation statements)

References 25 publications

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“…Interestilingly, in the present study these structures (OT, TS) exhibit [ 3 H]Ro 15-1788 labelling indicating that BZ receptor could be associated with this pathway. Similar to our binding study, the cerebellum of the saltwater fish Mullus surmuletus (Giannaccini et al, 1997) and of the Mugil cephalus (Betti et al, 2001) contained a low concentration of receptor sites. Autoradiographic investigations, using [ 3 H]flunitrazepan, in the cerebellum of chicken brain revealed a moderate degree of labelling (Aller et al, 2003).…”

Section: Discussionsupporting

confidence: 87%

“…The rank order of displacement efficacy of unlabelled ligands observed, suggested that in brain membranes of Cyprinus carpio central-type benzodiazepine receptors are present in one class of binding sites (Type I-lke), as found in Mullus surmuletus (Giannaccini et al, 1997) and in Mugil cephalus (Betti et al, 2001).…”

Section: Discussionmentioning

confidence: 92%

“…1b-g) displayed a high density of labelling, confirming the binding data. The presence of abudant BZ receptor sites in the OT was described in the teleost fish Mugil cephalus and Mullus surmuletus (Giannaccini et al, 1997;Betti et al, 2001). In chicken brain autoradiograms exhibited at level of the OT a high density of BZ binding sites (Aller et al, 2003).…”

Section: Discussionmentioning

confidence: 96%

“…Fish brain for its phylogenetic variability, and for its anatomical specializations, represents a valid model for neurobiological studies. In previous papers, we performed binding assays on the GABA/BZ receptors in the brain of two teleost fishes (Giannaccini et al, 1997;Betti et al, 2001). Here, we investigated the autoradiographic distribution and the binding properties of the GABA/BZ receptor in the brain of the carp (Cyprinus carpio L.), which showed a particular development of the neuroanatomical structures implicated in gustatory signal processing Finger, 1985, 1987).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Autoradiographic localization and binding study of benzodiazepines receptor sites in carp brain (Cyprinus carpio L.)

Pirone

Giannaccini

Betti

et al. 2006

Journal of Chemical Neuroanatomy

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Autoradiographic localization and binding study of benzodiazepines receptor sites in carp brain (Cyprinus carpio L.)

Pirone

Giannaccini

Betti

et al. 2006

Journal of Chemical Neuroanatomy

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“…A variety of studies have identified benzodiazepine-GABA-A receptors in fish and shown that they have similar binding characteristics as those in rodents and humans [1,2,7,13,32], though functionally they may have some differences from mammals [6]. There is evidence that benzodiazepine-GABA receptors are less pharmacologically responsive in fish, at least with regard to controlling convulsant activity [9].…”

Section: Introductionmentioning

confidence: 99%

Buspirone, chlordiazepoxide and diazepam effects in a zebrafish model of anxiety

Bencan

Sledge

Levin

2009

Pharmacology Biochemistry and Behavior

360

290

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Zebrafish are becoming more widely used to study neurobehavioral pharmacology. We have developed a method to assess novel environment diving behavior of zebrafish as a model of stress response and anxiolytic drug effects. In a novel tank, zebrafish dwell in the bottom of the tank initially and then increase their swimming exploration to higher levels as the session progresses. We previously found that nicotine, which has anxiolytic effects in rodents and humans, significantly lessens the novel tank diving response in zebrafish. The specificity of the diving effect was validated with a novel vs. non-novel test tank. The novel tank diving response zebrafish was tested when given three anxiolytic drugs from two different chemical and pharmacological classes: buspirone, chlordiazepoxide and diazepam. When the test tank was novel the diving response was clearly seen where as it was significantly reduced when the test tank was not novel. Buspirone, a serotonergic (5HT 1A receptor agonist) anxiolytic drug with some D 2 dopaminergic effect, had a pronounced anxiolytic-like effect in the zebrafish diving model at doses that did not have sedative effects. In contrast, chlordiazepoxide, a benzodiazepine anxiolytic drug, which is an effective agonist at GABA-A receptors, did not produce signs of anxiolysis in zebrafish over a broad dose range up to those that caused sedation. Diazepam another benzodiazepine anxiolytic drug did produce an anxiolytic effect at doses that did not cause sedation. The zebrafish novel tank diving task can be useful in discriminating anxiolytic drugs of several classes (serotonergic, benzodiazepines and nicotinic).

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