3H-Clonidine binding to membranes of platelets prepared under physiological conditions

Auffarth, Susan E.; Steiner, Meir; Werstiuk, Eva S.

doi:10.1016/0278-5846(85)90020-x

Cited by 3 publications

(2 citation statements)

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“…Under these experimental conditions it is the high affinity state of the a2adrenergic receptor that is labelIed by 3H-c1onidine (Mean KJ values: 1.71 ± 1.26 and 1.87 ± 1.54 nM in controls and untreated patients, respectively). The Smax values in our study were approximately 2-3 limes higher than those reported by other groups (Mean Smax values 134 ± 77 and 156 ± 88 fmol/mg protein in controls and patients, respectively), suggesting that during our experimental conditions (using intact platelets, isolated under physiological conditions, and binding assay term inated by centrifugation) (Auffarth et al, 1985) a greater number of a2-adrenergic receptor sites are maintained in the high affinity state.…”

Section: Discussioncontrasting

confidence: 67%

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Platelet α₂-Adrenergic Receptors in Depressed Patients and Healthy Volunteers: The Effects of Desipramine

Werstiuk

Auffarth²,

Coote³

et al. 1992

Pharmacopsychiatry

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Binding parameters (Bmax and Kd) of alpha 2-adrenergic receptors were studied in platelets from 14 depressed patients and 18 control subjects. Using 3H-clonidine (a partial alpha 2-adrenergic agonist) as the ligand and membranes, prepared from platelets isolated under physiological conditions, we found no significant differences in Bmax and Kd between medication free patients and control subjects. Platelet binding parameters in the depressed patients did not correlate with plasma levels of norepinephrine, epinephrine or MHPG. Age had a significant positive effect on platelet alpha 2-adrenergic receptor Bmax in both groups, and may have masked the patient-control differences. Treatment with desipramine for 28 days had no effect on the binding parameters in depressed patients when compared to pretreatment values. Adding desipramine to platelets of control subjects 'in vitro' did also not affect binding parameters. Our findings suggest that receptor binding studies with a partial alpha 2-adrenergic agonist in platelet membranes are not a useful model to test the hypothesis of a central supersensitive adrenergic system in depression.

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Section: Discussioncontrasting

confidence: 67%

“…Protein detenninations were perfonned according to the method of Lowry et al (1951). The binding parameters Kd and B max were calculated using the program Ligand (Munson and Rodbard, 1980) and the data were analyzed to fit a one site model (for details see Auffarth et al, 1985).…”

Section: Binding Assaysmentioning

confidence: 99%

Platelet α₂-Adrenergic Receptors in Depressed Patients and Healthy Volunteers: The Effects of Desipramine

Werstiuk

Auffarth²,

Coote³

et al. 1992

Pharmacopsychiatry

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Platelet alpha2A-adrenoceptor function in major depression: Gi coupling, effects of imipramine and relationship to treatment outcome

Gurguis

Griffith

et al. 1999

Psychiatry Research

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Effects of Electroconvulsive Therapy on Peripheral Adrenoceptors, Plasma, Noradrenaline, MHPG and Cortisol in Depressed Patients

et al. 1996

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3H-Clonidine binding to membranes of platelets prepared under physiological conditions

Cited by 3 publications

References 8 publications

Platelet α₂-Adrenergic Receptors in Depressed Patients and Healthy Volunteers: The Effects of Desipramine

Platelet α₂-Adrenergic Receptors in Depressed Patients and Healthy Volunteers: The Effects of Desipramine

Platelet alpha2A-adrenoceptor function in major depression: Gi coupling, effects of imipramine and relationship to treatment outcome

Effects of Electroconvulsive Therapy on Peripheral Adrenoceptors, Plasma, Noradrenaline, MHPG and Cortisol in Depressed Patients

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3H-Clonidine binding to membranes of platelets prepared under physiological conditions

Cited by 3 publications

References 8 publications

Platelet α2-Adrenergic Receptors in Depressed Patients and Healthy Volunteers: The Effects of Desipramine

Platelet α2-Adrenergic Receptors in Depressed Patients and Healthy Volunteers: The Effects of Desipramine

Platelet alpha2A-adrenoceptor function in major depression: Gi coupling, effects of imipramine and relationship to treatment outcome

Effects of Electroconvulsive Therapy on Peripheral Adrenoceptors, Plasma, Noradrenaline, MHPG and Cortisol in Depressed Patients

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Product

Resources

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Platelet α₂-Adrenergic Receptors in Depressed Patients and Healthy Volunteers: The Effects of Desipramine

Platelet α₂-Adrenergic Receptors in Depressed Patients and Healthy Volunteers: The Effects of Desipramine