Objective: In order to explore how to change the transverse tension transmission of skeletal muscle, a small injury model of skeletal muscle caused by Wushu was established. Methods: 36 healthy Wushu training athletes were randomly assigned to the experimental group (EG, n = 27) and the control group (CG, n = 9). The CG group ate normally and did not do any kind of sports; the EG group let martial arts athletes do sports training, especially for skeletal muscle training. After 30 minutes of training, the skin and muscle tissues of EG group were extracted at 1 h, 48 h and 120 h, respectively, and were used to measure the changes of transverse tension transmission in vivo, and to observe the ultrastructures of skeletal muscle, the structure of extracellular matrix between adjacent cells, and the changes of the junction sites between the small branches of collagen fiber network of myofascicular membrane and myointima and myomembrane. Results: Compared with the control group, the changes of the total transverse tension transfer of EG and CG in the experimental group were larger at 1H, 24h and 48h, showing the trend of first rising, then falling and then rising again; the micro damage of skeletal muscle, collagen area and the number of PJPs in the experimental group were significantly increased and showed the trend of first rising and then falling, which was statistically significant (P < 0.05). Conclusion: The micro damage of skeletal muscle caused by martial arts increased, the number of muscle bundle membrane links increased, at the same time, the ability of transverse tension transmission of skeletal muscle increased.