3D tissue engineering, an emerging technique for pharmaceutical research

Jensen, Gregory C.; Morrill, Christian; Huang, Yu

doi:10.1016/j.apsb.2018.03.006

Cited by 58 publications

(43 citation statements)

References 119 publications

(181 reference statements)

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“…Our 3D human cell culture model is vital for studying many human CNS diseases, which is inaccessible in 2D cultures 26 . To improve the use of 3D scaffolds for drug screening and drug delivery purposes, it is required to alter the chemical and biophysical properties of the materials to better suit of the needs of the cultured cells and native tissues 27 . Taking traumatic brain injury (TBI) as an instance, we showed that human cortical neurons responded to physiologically relevant impact forces.…”

Section: Discussionmentioning

confidence: 99%

Develop a 3D neurological disease model of human cortical glutamatergic neurons using micropillar-based scaffolds

Chen

Dong

Fang

et al. 2019

Acta Pharmaceutica Sinica B

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Establishing an effective three-dimensional (3D) in vitro culture system to better model human neurological diseases is desirable, since the human brain is a 3D structure. Here, we demonstrated the development of a polydimethylsiloxane (PDMS) pillar-based 3D scaffold that mimicked the 3D microenvironment of the brain. We utilized this scaffold for the growth of human cortical glutamatergic neurons that were differentiated from human pluripotent stem cells. In comparison with the 2D culture, we demonstrated that the developed 3D culture promoted the maturation of human cortical glutamatergic neurons by showing significantly more MAP2 and less Ki67 expression. Based on this 3D culture system, we further developed an in vitro disease-like model of traumatic brain injury (TBI), which showed a robust increase of glutamate-release from the neurons, in response to mechanical impacts, recapitulating the critical pathology of TBI. The increased glutamate-release from our 3D culture model was attenuated by the treatment of neural protective drugs, memantine or nimodipine. The established 3D in vitro human neural culture system and TBI-like model may be used to facilitate mechanistic studies and drug screening for neurotrauma or other neurological diseases.

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Section: Discussionmentioning

confidence: 99%

Develop a 3D neurological disease model of human cortical glutamatergic neurons using micropillar-based scaffolds

Chen

Dong

Fang

et al. 2019

Acta Pharmaceutica Sinica B

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“…Additional functional groups such as targeting ligands 48 , enzyme-responsive groups49, 50, 51, drugs52, 53, fatty acids54, 55, DNA 56 , chelator 57 , fluorophores 58 can be integrated into peptide gelators without disturbing their capability in hydrogel formation. These peptide-based hydrogels have found broad biomedical applications in areas, such as tissue engineering 59 , drug delivery 60 and imaging58, 61.…”

Section: Introductionmentioning

confidence: 99%

Injectable peptide hydrogel as intraperitoneal triptolide depot for the treatment of orthotopic hepatocellular carcinoma

Zhao

Liu

Zhang

et al. 2019

Acta Pharmaceutica Sinica B

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Chemotherapy is among the limited choices approved for the treatment of hepatocellular carcinoma (HCC) at intermediate and advanced stages. Preferential and prolonged drug exposure in diseased sites is required to maximize the therapeutic index of the drug. Here, we report an injectable supramolecular peptide hydrogel as an intraperitoneal depot for localized and sustained release of triptolide for the treatment of orthotopic HCC. We chose peptide amphiphile C16-GNNQQNYKD-OH-based nanofibers as gelators and carriers for triptolide. Sustained triptolide release from the hydrogel was achieved over 14 days in vitro, with higher accumulation in and cytotoxicity against human HCC Bel-7402 in comparison with L-02 fetal hepatocytes. After intraperitoneal injection, the hydrogel showed prolonged retention over 13 days and preferential accumulation in the liver, realizing HCC growth inhibition by 99.7 ± 0.1% and animal median survival extension from 19 to 43 days, without causing noticeable pathological changes in the major organs. These results demonstrate that injectable peptide hydrogel can be a potential carrier for localized chemotherapy of HCC.

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“…Indeed, by using biocompatible materials in the form of three-dimensional (3D) scaffolds for cell growth and differentiation, it is possible to obtain in vitro biological constructs that resemble, in terms of morphology and function, the native tissues more closely than conventional bi-dimensional (2D) cell cultures [14]. As a consequence, 3D tissue-engineered constructs represent valuable models to assess the effect of new drugs in a more reliable manner [15]. A number of biomaterials and technologies have been used to create TE scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Waste Autochthonous Tuscan Olive Leaves (Olea europaea var. Olivastra seggianese) as Antioxidant Source for Biomedicine

Ossa

Felice

Azimi

et al. 2019

IJMS

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Olive leaf extract (OLE) can be obtained as biowaste and is extensively used a food supplement and an over-the-counter drug for its beneficial effects. New studies have investigated OLE concerning the role of oxidative stress in the pathogenesis of vascular disease. This in vitro study aims to evaluate if OLE extracted from the Tuscan Olea europaea protects endothelial cells against oxidative stress generated by reactive oxygen species (ROS). Methods: OLE total polyphenols (TPs) were characterized by the Folin–Ciocalteu method. Endothelial cells were grown in conventional cultures (i.e., two-dimensional, 2D) and on a biomaterial scaffold (i.e., three-dimensional, 3D) fabricated via electrospinning. Cell viability and ROS measurement after H2O2 insults were performed. Results: OLE TP content was 23.29 mg GAE/g, and oleuropein was the principal compound. The dose-dependent viability curve highlighted the absence of significant cytotoxic effects at OLE concentrations below 250 µg/mL TPs. By using OLE preconditioning at 100 µg/mL, cell viability decrease was observed, being in 3D lower than in the 2D model. OLE was protective against ROS in both models. Conclusions: OLE represents a high-value antioxidant source obtained by biowaste that is interesting for biomedical products. Using a 3D scaffold could be the best predictive model to mimic the physiological conditions of vascular tissue reaction.

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3D tissue engineering, an emerging technique for pharmaceutical research

Cited by 58 publications

References 119 publications

Develop a 3D neurological disease model of human cortical glutamatergic neurons using micropillar-based scaffolds

Develop a 3D neurological disease model of human cortical glutamatergic neurons using micropillar-based scaffolds

Injectable peptide hydrogel as intraperitoneal triptolide depot for the treatment of orthotopic hepatocellular carcinoma

Waste Autochthonous Tuscan Olive Leaves (Olea europaea var. Olivastra seggianese) as Antioxidant Source for Biomedicine

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