3D representation of biostructures imaged with an optical microscope

Diaspro, Alberto; Sartore, Marco; Nicolini, Claudio

doi:10.1016/0262-8856(90)90028-4

Cited by 32 publications

(13 citation statements)

References 28 publications

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“…Image acquisition and three-dimensional montage software were performed with C-Imaging software (Compix Inc., Cranberry Township, PA), and some processing was performed with IMAGO VII (16). Cell area was measured with C-Imaging software.…”

Section: Confocal Imagingmentioning

confidence: 99%

Cytofluorometry and fluorescence confocal laser scanning microscopy (CLSM) in the study of neutral lipid dynamics inParamecium primaurelia mating types during cell line development

et al. 1999

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Section: Confocal Imagingmentioning

confidence: 99%

Cytofluorometry and fluorescence confocal laser scanning microscopy (CLSM) in the study of neutral lipid dynamics inParamecium primaurelia mating types during cell line development

et al. 1999

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“…This method, known as optical sectioning (Weinstein and Castleman, 1971;Agard, 1984), is based on the acquisition of data at different planes of focus by means of an appropriate device. Its modern form is established in confocal and two-photon microscopy techniques (Diaspro, 2001), as well as in computational methods of datasets coming from conventional microscopy operating under defocused conditions (Agard, 1984;Bianco and Diaspro, 1989;Diaspro et al, 1990). When the microscope is in focus on a specific plane of a given object, the image being acquired is obtained from the joined contribution of the plane that is in focus as well as the other planes.…”

Section: Image Formation and Processingmentioning

confidence: 99%

Three‐dimensional microscopy migrates to the web with “PowerUp Your Microscope”

Bonetto

Boccacci

Scarito³

et al. 2004

Microscopy Res & Technique

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"PowerUp Your Microscope" is a software package designed and realized for the optimization of 3D optical microscopy image quality using the Internet and inverse problems computational approaches. The package is mainly devoted to 3D microscopy users, being operative for wide-field, confocal, and multiphoton microscopy. It provides the microscopy community with an extremely easy and comparatively powerful access to advanced image restoration methods. The core of the computational section is the optical system modeling and inverse deconvolution implementation, which is strongly linked to Web-based software and technology. This project constitutes a real and effective migration to the Web, extending computational approaches to image restoration to the whole microscopy user community, regardless of their background.

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“…In confocal microscopy, only in-focus light is imaged, whereas out-of-focus light is rejected by the confocal aperture (pinhole). By scanning successive optical planes, a three-dimensional image of the sample can be obtained (Bianco and Diaspro, 1977;Brakenhoff et al, 1979;Diaspro et al, 1990;Sheppard, 1989;Wilson, 1989).…”

Section: Introductionmentioning

confidence: 99%

Adaptive image‐processing technique and effective visualization of confocal microscopy images

Sun

Rajwa

Robinson

2004

Microscopy Res & Technique

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A common observation about confocal microscopy images is that lower image stacks have lower voxel intensities and are usually blurred in comparison with the upper ones. The key reasons are light absorption and scattering by the objects and particles in the volume through which light passes. This report proposes a new technique to reduce such noise impacts in terms of an adaptive intensity compensation and structural sharpening algorithm. With these image-processing procedures, effective 3D rendering techniques can be applied to faithfully visualize confocal microscopy data.

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3D representation of biostructures imaged with an optical microscope

Cited by 32 publications

References 28 publications

Cytofluorometry and fluorescence confocal laser scanning microscopy (CLSM) in the study of neutral lipid dynamics inParamecium primaurelia mating types during cell line development

Cytofluorometry and fluorescence confocal laser scanning microscopy (CLSM) in the study of neutral lipid dynamics inParamecium primaurelia mating types during cell line development

Three‐dimensional microscopy migrates to the web with “PowerUp Your Microscope”

Adaptive image‐processing technique and effective visualization of confocal microscopy images

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