3D Pulsed Laser Triggered High Speed Microfluidic Fluorescence Activated Cell Sorter

Chen, Yue; Wu, Ting-Hsiang; Kung, Yu-Chun; Teitell, Michael A.; Chiou, Eric P. Y.

doi:10.1364/cleo_si.2013.cm1m.3

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…The microfluidic chip is based on a two-step 3D hydrodynamic focusing technique (Sakuma et al, 2017;Chen et al, 2013) to align cells at the center of the microchannel before the FDM microscope (Figures 1 and S1A). Since the flow speed distribution in the microchannel has a parabolic profile (i.e., the speed is maximum at the center), the position of cells in the cross section of the microchannel greatly affects not only the yield of image detection, but also the recovery of sorting.…”

Section: Hydrodynamic Focusingmentioning

confidence: 99%

Intelligent Image-Activated Cell Sorting

Nitta

Sugimura

Isozaki

et al. 2018

Cell

432

354

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A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.

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Section: Hydrodynamic Focusingmentioning

confidence: 99%

Intelligent Image-Activated Cell Sorting

Nitta

Sugimura

Isozaki

et al. 2018

Cell

432

354

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“…In the past two decades, there has been a surge in studies exploring micropump technologies [4][5][6]. In view of the need for rapid and accurate control of microfluidics in lab-on-chip applications, scholars have explored micropumps with multiple driving modes, including optically-driven pumps [7,8], electro-osmotic pumps [9,10], electrokinetic pumps [11,12], dielectric pumps [13,14], magnetic pumps [15,16], laser-driven pumps [17], pneumatic membrane pumps [18][19][20], bio-hybrid pumps [21,22], and diffuser pumps [23,24]. Recently, the acoustic streaming effect produced by acoustic waves in microfluids has attracted considerable interest, and several microdevices have been explored, including micromixers [25][26][27][28][29], particle manipulation [30][31][32][33][34], and flow control [35,36].…”

Section: Introductionmentioning

confidence: 99%

A Bi-Directional Acoustic Micropump Driven by Oscillating Sharp-Edge Structures

et al. 2023

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This paper proposes a bi-directional acoustic micropump driven by two groups of oscillating sharp-edge structures: one group of sharp-edge structures with inclined angles of 60° and a width of 40 μm, and another group with inclined angles of 45° and a width of 25 μm. One of the groups of sharp-edge structures will vibrate under the excitation of the acoustic wave generated with a piezoelectric transducer at its corresponding resonant frequency. When one group of sharp-edge structures vibrates, the microfluid flows from left to right. When the other group of sharp-edge structures vibrates, the microfluid flows in the opposite direction. Some gaps are designed between the sharp-edge structures and the upper surface and the bottom surface of the microchannels, which can reduce the damping between the sharp-edge structures and the microchannels. Actuated with an acoustic wave of a different frequency, the microfluid in the microchannel can be driven bidirectionally by the inclined sharp-edge structures. The experiments show that the acoustic micropump, driven by oscillating sharp-edge structures, can produce a stable flow rate of up to 125 μm/s from left to right, when the transducer was activated at 20.0 kHz. When the transducer was activated at 12.8 kHz, the acoustic micropump can produce a stable flow rate of up to 85 μm/s from right to left. This bi-directional acoustic micropump, driven by oscillating sharp-edge structures, is easy to operate and shows great potential in various applications.

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“…Thus, glass is mainly used in the microfluidic devices for applications requiring high pressure/high‐flow velocity and low‐background noise for observation, including high throughput cell manipulation, imaging, diagnosis, and therapy [4–6]. For example, in applications of high‐speed cell sorting and imaging [7, 8], they required high‐flow velocity, such as 10 m/s [9, 10], and strong/rigid wall for pressure resistance [11]. To achieve such velocity, it required high pressure applied to an inlet and a channel.…”

Section: Introductionmentioning

confidence: 99%

Horizontal connection method for glass microfluidic devices

Yalikun

Ota

et al. 2020

Micro & Nano Letters

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In a conventional glass microfluidic system, the vertical connection is the most frequently used for connections between a microfluidic device and its peripheral equipment. However, it leads to a significant pressure drop at the inlet of a microfluidic device, decreases the flow velocity, and can introduce few samples than expected. To overcome these problems, a horizontal connection method for glass microfluidic devices, which have advantages such as keeping pressure/flow velocity, compatible with small space, reduced sample loss and convenience for modularisation is reported. These advantages are important for high-speed analysis on precious samples such as single-cell flow cytometry. In this work, they proposed a horizontal connection method which showed compatibility with high pressure (at least 0.6 MPa) at inlet without leakage, small loss of injected samples (<0.11%), and 22% faster flow velocity along the direction of applied pressure than that of vertical connection.

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3D Pulsed Laser Triggered High Speed Microfluidic Fluorescence Activated Cell Sorter

Cited by 7 publications

References 27 publications

Intelligent Image-Activated Cell Sorting

Intelligent Image-Activated Cell Sorting

A Bi-Directional Acoustic Micropump Driven by Oscillating Sharp-Edge Structures

Horizontal connection method for glass microfluidic devices

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