3D printing of porous alginate/gelatin hydrogel scaffolds and their mechanical property characterization

You, Fei; Wu, Xiaoqing; Chen, Daniel

doi:10.1080/00914037.2016.1201830

Cited by 129 publications

(96 citation statements)

References 26 publications

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“…In some studies, only printing parameters were investigated as critical factors influencing printability [2,11]. In another study, the gelation properties of materials during the printing process were studied to achieve a mechanically stable structure [12]. Murphy et al studied gelation time, swelling, and the printability of various groups of hydrogels [13].…”

Section: Introductionmentioning

confidence: 99%

“…Gelatin is one of the hydrogels usually mixed with alginate. Gelatin is a natural polymer derived from collagen and it has a cell-friendly environment and this is one of the reasons for mixing alginate with this biomaterial [12]. Methylcellulose (MC) is another biocompatible hydrocarbon polymer commonly used in scaffold fabrication due to its high hydrophilicity and water absorption, essential for nutrient delivery to the cells [25].…”

Section: Introductionmentioning

confidence: 99%

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Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters

et al. 2019

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Extrusion-based bioprinting of hydrogel scaffolds is challenging due to printing-related issues, such as the lack of capability to precisely print or deposit hydrogels onto three-dimensional (3D) scaffolds as designed. Printability is an index to measure the difference between the designed and fabricated scaffold in the printing process, which, however, is still under-explored. While studies have been reported on printing hydrogel scaffolds from one or more hydrogels, there is limited knowledge on the printability of hydrogels and their printing processes. This paper presented our study on the printability of 3D printed hydrogel scaffolds, with a focus on identifying the influence of hydrogel composition and printing parameters/conditions on printability. Using the hydrogels synthesized from pure alginate or alginate with gelatin and methyl-cellulose, we examined their flow behavior and mechanical properties, as well as their influence on printability. To characterize the printability, we examined the pore size, strand diameter, and other dimensions of the printed scaffolds. We then evaluated the printability in terms of pore/strand/angular/printability and irregularity. Our results revealed that the printability could be affected by a number of factors and among them, the most important were those related to the hydrogel composition and printing parameters. This study also presented a framework to evaluate alginate hydrogel printability in a systematic manner, which can be adopted and used in the studies of other hydrogels for bioprinting.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters

et al. 2019

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“…However, absorption of gelatin molecules released from the indirectly-fabricated framework on the surface of alginate strands might enhance the surface properties of alginate scaffolds fabricated by the proposed indirect approach. Moreover, some amount of gelatin might get absorbed in the alginate hydrogel during the removal process, and thereafter have a positive effect on the cellular behavior (You et al, 2016).…”

Section: Cell Morphology In Bulk Gels and Cell-incorporated/post-seedmentioning

confidence: 99%

Indirect 3D bioprinting and characterization of alginate scaffolds for potential nerve tissue engineering applications

Naghieh

Sarker

Abelseth

et al. 2019

Journal of the Mechanical Behavior of Biomedical Materials

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Saman Naghieh) and xbc719@mail.usask.ca (Xiongbiao Chen). AbstractLow-concentration hydrogels have favorable properties for many cell functions in tissue engineering but are considerably limited from a scaffold fabrication point of view due to poor three-dimensional (3D) printability. Here, we developed an indirect-bioprinting process for alginate scaffolds and characterized the potential of these scaffolds for nerve tissue engineering applications. The indirect-bioprinting process involves (1) printing a sacrificial framework from gelatin, (2) impregnating the framework with low-concentration alginate, and (3) removing the gelatin framework by an incubation process, thus forming low-concentration alginate scaffolds. The scaffolds were characterized by compression testing, swelling, degradation, and morphological and biological assessment of incorporated or seeded Schwann cells. For comparison, varying concentrations of alginate scaffolds (from 0.5 to 3%) were fabricated and sterilized using either ultraviolet light or ethanol. Results indicated that scaffolds can be fabricated using the indirect-bioprinting process, wherein the scaffold properties are affected by 5 the concentration of alginate and sterilization technique used. These factors provide effective means of regulating the properties of scaffolds fabricated using the indirect-bioprinting process.Cell-incorporated scaffolds demonstrated better cell viability than bulk gels. In addition, scaffolds showed better cell functionality when fabricated with a lower concentration of alginate compared to a higher concentration. The indirect-bioprinting process that we implemented could be extended to other types of low-concentration hydrogels to address the tradeoffs between printability and properties for favorable cell functions.

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“…The surfaces of the struts can be constantly indurated, and the stability of the scaffold increases through the continuous crosslinking of the previously printed layers. This method formed a 3D structure easy and more consistently than the submerged crosslinking technique, since the submerged process contained a high possibility of the bioink floating in the crosslinking solution during the printing process and required additional treatment, such as polyethylenimine (PEI) surface coating [42,47] or a layer-by-layer interactively moving stage [39][40][41]43] .…”

Section: D Cell Printing With Modified Crosslinking Processesmentioning

confidence: 99%

Recent cell printing systems for tissue engineering

Lee¹,

Koo²,

Yeo³

et al. 2017

ijb

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Three-dimensional (3D) printing in tissue engineering has been studied for the bio mimicry of the structures of human tissues and organs. Now it is being applied to 3D cell printing, which can position cells and biomaterials, such as growth factors, at desired positions in the 3D space. However, there are some challenges of 3D cell printing, such as cell damage during the printing process and the inability to produce a porous 3D shape owing to the embedding of cells in the hydrogel-based printing ink, which should be biocompatible, biodegradable, and non-toxic, etc. Therefore, researchers have been studying ways to balance or enhance the post-print cell viability and the print-ability of 3D cell printing technologies by accommodating several mechanical, electrical, and chemical based systems. In this mini-review, several common 3D cell printing methods and their modified applications are introduced for overcoming deficiencies of the cell printing process.

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3D printing of porous alginate/gelatin hydrogel scaffolds and their mechanical property characterization

Cited by 129 publications

References 26 publications

Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters

Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters

Indirect 3D bioprinting and characterization of alginate scaffolds for potential nerve tissue engineering applications

Recent cell printing systems for tissue engineering

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