3D Printing of Poly(3‐hydroxybutyrate) Porous Structures Using Selective Laser Sintering

Pereira, Tatiana; Oliveira, Marcelo Fernandes de; Maia, Ivan Ferrer; Silva, Jorge Vicente Lopes da; Costa, Marysilvia Ferreira da; Thiré, Rossana Mara da Silva Moreira

doi:10.1002/masy.201100237

Cited by 41 publications

(33 citation statements)

References 14 publications

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“…In the fourth and last step, PHB secreted by bacteria is extracted from the liquid harvest stream using dissolved air flotation. After drying, the PHB can be used in a Selective Laser Sintering (SLS) 3D printer without additional processing [14]. Each step of the process is further explained in subsequent sections.…”

Section: Process Overviewmentioning

confidence: 99%

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Astroplastic: A start-to-finish process for polyhydroxybutyrate production from solid human waste using genetically engineered bacteria to address the challenges for future manned Mars missions

Chen

Ibrahim

Kunitskaya

et al. 2018

Preprint

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Space exploration has long been a source of inspiration, challenging scientists and engineers to find innovative solutions to various problems. One of the current focuses in space exploration is to send humans to Mars. However, the challenge of transporting materials to Mars and the need for waste management processes are two major obstacles for these long-duration missions.To address these two challenges a process called Astroplastic was developed that produces polyhydroxybutyrate (PHB) from solid human waste, which can be used to 3D print useful items for astronauts. PHB granules are naturally produced by bacteria such as Ralstonia eutropha and Pseudomonas aeruginosa for carbon and energy storage. The phaJ, phaC, and phaCBA genes were cloned from these native PHB-producing bacteria into Escherichia coli. These genes code for enzymes that aid in PHB production by converting products of glycolysis and β -oxidation pathways, such as acetyl-CoA and enoyl-CoA, into PHB. To ensure a continuous PHB production system and to eliminate the need for cell lysis to extract PHB, recombinant E. coli was engineered to use the genes in its natural type I secretion system to secrete PHB. The C-terminal of the HlyA secretion tag was fused to phasin (PhaP), a protein originally from R. eutropha. Phasin-HlyA electrostatically binds PHB granules and transports them outside of the cell.In addition to genetically engineering bacteria, a concept for start-to-finish PHB production process was designed.Integrating expert feedback and experimental results, conditions for each step of the process including the collection and storage of waste, volatile fatty acid (VFA) fermentation, VFA extraction, PHB fermentation, and PHB extraction were optimized. The optimized system will provide a sustainable and continuous PHB production system, which will address the problems of transportation costs and waste management for future space missions.

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Section: Process Overviewmentioning

confidence: 99%

“…Lastly, the resulting PHB is extracted from the liquid harvest stream. The PHB can then be used in a Selective Laser Sintering (SLS) 3D printer without the need for additional processing [14]. Made in Space, Inc. is the company that developed and implemented the installation of a 3D printer currently in use on the ISS.…”

Section: Introductionmentioning

confidence: 99%

Astroplastic: A start-to-finish process for polyhydroxybutyrate production from solid human waste using genetically engineered bacteria to address the challenges for future manned Mars missions

Chen

Ibrahim

Kunitskaya

et al. 2018

Preprint

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“…In SLS, one of the many 3D printing technologies available, the slices are written by a carbon dioxide (CO 2 ) laser beam. The written process derives from the coalescence of particles through sinterization caused by the application of laser and thermal energy [16,17]. The slice geometries are defined by selective sintering that follows the laser beam scanning onto the powder layers, according to the digital slices.…”

Section: The Principle Of 3d Printing Technologymentioning

confidence: 99%

3D-Printed Biopolymers for Tissue Engineering Application

Cui

Sun

et al. 2014

International Journal of Polymer Science

141

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3D printing technology has recently gained substantial interest for potential applications in tissue engineering due to the ability of making a three-dimensional object of virtually any shape from a digital model. 3D-printed biopolymers, which combine the 3D printing technology and biopolymers, have shown great potential in tissue engineering applications and are receiving significant attention, which has resulted in the development of numerous research programs regarding the material systems which are available for 3D printing. This review focuses on recent advances in the development of biopolymer materials, including natural biopolymer-based materials and synthetic biopolymer-based materials prepared using 3D printing technology, and some future challenges and applications of this technology are discussed.

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“…SLS uses a carbon dioxide laser to coalesce and thereby sinterize the printing material through the application of both thermal and laser energy. The powdered printer material is thereby heated to melting point causing the particles to fuse together to form a shape in a thin slice-by-slice manner [2]. DMLS is a similar process that uses an Ytterbium laser to fuse metal powder into a solid part by melting it locally.…”

Section: Introductionmentioning

confidence: 99%

The Role of 3D Modelling and Printing in Orthopaedic Tissue Engineering: A Review of the Current Literature

Shaunak

Dhinsa²,

Khan

2017

CSCR

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Orthopaedic surgery lends itself well to advances in technology. An area of interest and ongoing research is that of the production of scaffolds for use in trauma and elective surgery. 3D printing provides unprecedented accuracy in terms of micro- and macro-structure and geometry for scaffold production. It can also be utilised to construct scaffolds of a variety of different materials and more recently has allowed for the construction of bio-implants which recapitulate bone and cartilage tissue. This review seeks to look at the various methods of 3DP, the materials used, elements of functionality and design, as well as modifications to increase the biomechanics and bioactivity of 3DP scaffolds.

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3D Printing of Poly(3‐hydroxybutyrate) Porous Structures Using Selective Laser Sintering

Cited by 41 publications

References 14 publications

Astroplastic: A start-to-finish process for polyhydroxybutyrate production from solid human waste using genetically engineered bacteria to address the challenges for future manned Mars missions

Astroplastic: A start-to-finish process for polyhydroxybutyrate production from solid human waste using genetically engineered bacteria to address the challenges for future manned Mars missions

3D-Printed Biopolymers for Tissue Engineering Application

The Role of 3D Modelling and Printing in Orthopaedic Tissue Engineering: A Review of the Current Literature

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