3D Printing of Lotus Root‐Like Biomimetic Materials for Cell Delivery and Tissue Regeneration

Feng, Chun; Zhang, Wenjie; Deng, Chengbin; Li, Guanglong; Chang, Jiang; Zhang, Zhiyuan; Jiang, Xinquan; Wu, Chengtie

doi:10.1002/advs.201700401

Cited by 170 publications

(117 citation statements)

References 46 publications

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“…[29] In Vitro Osteogenic Differentiation Analysis of Encapsulated BMSCS: To evaluate the proliferation of encapsulated BMSCs, spheroids were cultured in medium supplemented with EdU (diluted 1:1000, 1 mg L −1 ; Ribobio, China). BMSCs were suspended in medium with a high Mg 2+ concentration.…”

Section: Methodsmentioning

confidence: 99%

A Magnesium‐Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration

et al. 2019

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The redevelopment/regeneration pattern of amputated limbs from a blastema in salamander suggests that enhanced regeneration might be achieved by mimicking the developmental microenvironment. Inspired by the discovery that the expression of magnesium transporter‐1 (MagT1), a selective magnesium (Mg) transporter, is significantly upregulated in the endochondral ossification region of mouse embryos, a Mg‐enriched 3D culture system is proposed to provide an embryonic‐like environment for stem cells. First, the optimum concentration of Mg ions (Mg 2+ ) for creating the osteogenic microenvironment is screened by evaluating MagT1 expression levels, which correspond to the osteogenic differentiation capacity of stem cells. The results reveal that Mg 2+ selectively activates the mitogen‐activated protein kinase/extracellular regulated kinase (MAPK/ERK) pathway to stimulate osteogenic differentiation, and Mg 2+ influx via MagT1 is profoundly involved in this process. Then, Mg‐enriched microspheres are fabricated at the appropriate size to ensure the viability of the encapsulated cells. A series of experiments show that the Mg‐enriched microenvironment not only stimulates the osteogenic differentiation of stem cells but also promotes neovascularization. Obvious vascularized bone regeneration is achieved in vivo using these Mg‐enriched cell delivery vehicles. The findings suggest that biomaterials mimicking the developmental microenvironment might be promising tools to enhance tissue regeneration.

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Section: Methodsmentioning

confidence: 99%

A Magnesium‐Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration

et al. 2019

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“…The compressive modulus was defined as the slope of the initial linear regions of the stress-strain curves. The porosity of the scaffolds was investigated by the Archimedes' method as described previously (12). The surface roughness of the scaffolds was measured with a contact surface profilometer (Daktak-XT, Bruker, Germany) at a speed of 0.05 mm/s and a scanning length of 1 mm.…”

Section: Materials Characterizationmentioning

confidence: 99%

3D printing of Haversian bone–mimicking scaffolds for multicellular delivery in bone regeneration

et al. 2020

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The integration of structure and function for tissue engineering scaffolds is of great importance in mimicking native bone tissue. However, the complexity of hierarchical structures, the requirement for mechanical properties, and the diversity of bone resident cells are the major challenges in constructing biomimetic bone tissue engineering scaffolds. Herein, a Haversian bone–mimicking scaffold with integrated hierarchical Haversian bone structure was successfully prepared via digital laser processing (DLP)–based 3D printing. The compressive strength and porosity of scaffolds could be well controlled by altering the parameters of the Haversian bone–mimicking structure. The Haversian bone–mimicking scaffolds showed great potential for multicellular delivery by inducing osteogenic, angiogenic, and neurogenic differentiation in vitro and accelerated the ingrowth of blood vessels and new bone formation in vivo. The work offers a new strategy for designing structured and functionalized biomaterials through mimicking native complex bone tissue for tissue regeneration.

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“…The tailorable properties of biomaterials such as their chemical composition, surface properties, and topographical structures, can act as insoluble exogenous stimuli to promote adhesion and activation of specific cellular signaling pathways, as well as to direct MSC differentiation toward osteoblasts or other lineages . Recently, our group developed novel hydrogels from small peptide gelators containing an RGD ligand and silk fibroin (SF) .…”

Section: Introductionmentioning

confidence: 99%

Enhanced Osteogenesis of Bone Marrow‐Derived Mesenchymal Stem Cells by a Functionalized Silk Fibroin Hydrogel for Bone Defect Repair

Yan

Cheng

Chen

et al. 2018

Adv Healthcare Materials

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effective for bone defect treatment and bone reconstruction, these approaches are limited in their clinical application by certain constraints such as i) tissue morbidity at donor sites and the limited availability of autologous bone as well as ii) the high risk of infection and immunogenic rejection with allografts. [5][6][7][8][9] To overcome the limitations of autografting and allografting, various materials, including metals, ceramics, and polymers, have been investigated as bone scaffold biomaterials for bone graft substitutes. [10][11][12] In some cases, these scaffolds often suffer from low cell adhesion, insufficient bioactivity, or uncontrolled degradability. Therefore, the development of new classes of biomaterials for bone regeneration has been the focus of research in the fields of bone tissue engineering. [13][14][15] Ideal biomaterials for bone regeneration should provide temporary structural scaffolds with suitable mechanical strength and possess bioactive elements for cell adhesion, proliferation, differentiation of tissue regenerating cells, and the generation of a new mineralized bone matrix. [16,17] Over the last decade, hydrogels have emerged as a promising class of biomaterials for the fabrication of both bone scaffolds and bone-engineering tissues due to their high water content, structural similarity to natural extracellular matrices (ECM), [18][19][20][21] and tailorable physical and biochemical properties. [22] In contrast to rigid biomaterials from ceramics and metals, hydrogels exhibit a high degree of flexibility and toughness. Therefore, after implantation via minimally invasive surgical techniques, hydrogels are capable of fitting the lesion geometry of defects and establish tight contacts with host tissues to achieve better cell adhesion and therapy. [23][24][25] Moreover, recent advances in the development of cell-laden hydrogels have opened up a new opportunity for bone repair and regeneration. [26][27][28] Hydrogels act as 3D scaffolds providing suitable microenvironments for cell proliferation, differentiation, and maintenance of function, thus allowing exogenous cells to grow and secrete new ECM for the reconstruction of damaged bone tissues. [29,30] Mesenchymal stem cells (MSCs) are multipotent stromal cells originating from umbilical cord, muscle, and bone Silk fibroin (SF) from Bombyx mori is a promising natural material for the synthesis of biocompatible and biodegradable hydrogels for use in biomedical applications from tissue engineering to drug delivery. However, weak gelation performance and the lack of biochemical cues to trigger cell proliferation and differentiation currently significantly limit its application in these areas. Herein, a biofunctional hydrogel containing SF (2.0%) and a small peptide gelator (e.g., NapFFRGD = 1.0 wt%) is generated via cooperative molecular self-assembly. The introduction of NapFFRGD to SF is shown to significantly improve its gelation properties by lowering both its threshold gelation concentration to 2.0% and gelation time to 20 min ...

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3D Printing of Lotus Root‐Like Biomimetic Materials for Cell Delivery and Tissue Regeneration

Cited by 170 publications

References 46 publications

A Magnesium‐Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration

A Magnesium‐Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration

3D printing of Haversian bone–mimicking scaffolds for multicellular delivery in bone regeneration

Enhanced Osteogenesis of Bone Marrow‐Derived Mesenchymal Stem Cells by a Functionalized Silk Fibroin Hydrogel for Bone Defect Repair

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