3D printing of functional nerve guide conduits

Huang, Yulan; Wu, Wenbi; Liu, Haofan; Chen, Yu‐Wen; Li, Bo; Gou, Zhiyuan; Li, Xun; Gou, Maling

doi:10.1093/burnst/tkab011

Cited by 22 publications

(14 citation statements)

References 118 publications

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“…This is one limitation of our branch nerve conduit. However, an increasing number of studies have found that functional nerve conduits can promote nerve regeneration [ 22 , 23 ]. For instance, we previously found that cell-loaded or drug-loaded nerve conduits were superior to nonfunctional nerve conduits and had regeneration ability comparable to that of autografts [ 15 , 16 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Nerve transfer with 3D-printed branch nerve conduits

et al. 2022

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Background Nerve transfer is an important clinical surgical procedure for nerve repair by the coaptation of a healthy donor nerve to an injured nerve. Usually, nerve transfer is performed in an end-to-end manner, which will lead to functional loss of the donor nerve. In this study, we aimed to evaluate the efficacy of 3D-printed branch nerve conduits in nerve transfer. Methods Customized branch conduits were constructed using gelatine-methacryloyl by 3D printing. The nerve conduits were characterized both in vitro and in vivo. The efficacy of 3D-printed branch nerve conduits in nerve transfer was evaluated in rats through electrophysiology testing and histological evaluation. Results The results obtained showed that a single nerve stump could form a complex nerve network in the 3D-printed multibranch conduit. A two-branch conduit was 3D printed for transferring the tibial nerve to the peroneal nerve in rats. In this process, the two branches were connected to the distal tibial nerve and peroneal nerve. It was found that the two nerves were successfully repaired with functional recovery. Conclusions It is implied that the two-branch conduit could not only repair the peroneal nerve but also preserve partial function of the donor tibial nerve. This work demonstrated that 3D-printed branch nerve conduits provide a potential method for nerve transfer.

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Section: Discussionmentioning

confidence: 99%

Nerve transfer with 3D-printed branch nerve conduits

et al. 2022

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“…Since remnant particles may cause a foreign body reaction, [266] DIW allows continuous printing without remnant particles on the printing bed attractively. To obtain a more accurate bifurcated Yshaped NGC or more permeable NGC for hindering neuroma formation, [268][269][270] imaging technology and SLIS [210] can apace custom the model. Besides, selective laser sintering (SLS) [208,220] has been widely used for high mechanical strength scaffolds in bone tissue engineering, but it is rarely applied to fabricate NGC.…”

Section: D Printingmentioning

confidence: 99%

Three Potential Elements of Developing Nerve Guidance Conduit for Peripheral Nerve Regeneration

Zhang,

Gong,

Zhang

et al. 2023

Adv Funct Materials

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Autograft replaced by a nerve guidance conduit (NGC) is challenging in peripheral nerve injury because current NGC is still limited by precise conductivity and excellent biocompatibility in vivo, which influences the peripheral nerve repair even for a long lesion gap repair. Several particular elements have the potential function for nerve conductivity acceleration based on the traditional three factors of neural tissue engineering. The review aims to address three questions: 1) What is the superior factor for nerve conduction in the application? 2) How can a more conductive regenerative scaffold be constructed in vivo? 3) What is the next step in nerve regeneration for NGC? The bibliometrics analysis of NGC‐related references is adopted to acquire that the conductive material, manufacturing technology of neural scaffold, and electrical stimulation (ES) play essential roles in the acceleration of nerve conduction. This review visually analyses the research status and summarizes the main types of conductive materials, the manufacturing technologies of neural scaffolds, and the characteristics of ES. The viewpoints and outlook of developing NGC are also discussed in this review. The proposed three elements are expected to improve the nerve conduction of NGC in vivo and even address the dilemma of long‐distance peripheral nerve injury.

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“…Currently, indirect 3D bioprinting has made progress in both hard and soft tissue engineering systems. Some specific tissue constructs such as functional nerve guide conduits, human knee meniscal scaffolds, sized vascular grafts, can be successfully produced in laboratories as proof of concept (Schöneberg et al, 2018;Huang et al, 2021;P B et al, 2022). At the same time, with the assistance of sacrificial molds, materials that are traditionally difficult to print can be utilized to manufacture fine structures.…”

Section: Indirect 3d Bioprinting For Tissue Vascularization Vasculari...mentioning

confidence: 99%

Biodegradable Inks in Indirect Three-Dimensional Bioprinting for Tissue Vascularization

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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Mature vasculature is important for the survival of bioengineered tissue constructs, both in vivo and in vitro; however, the fabrication of fully vascularized tissue constructs remains a great challenge in tissue engineering. Indirect three-dimensional (3D) bioprinting refers to a 3D printing technique that can rapidly fabricate scaffolds with controllable internal pores, cavities, and channels through the use of sacrificial molds. It has attracted much attention in recent years owing to its ability to create complex vascular network-like channels through thick tissue constructs while maintaining endothelial cell activity. Biodegradable materials play a crucial role in tissue engineering. Scaffolds made of biodegradable materials act as temporary templates, interact with cells, integrate with native tissues, and affect the results of tissue remodeling. Biodegradable ink selection, especially the choice of scaffold and sacrificial materials in indirect 3D bioprinting, has been the focus of several recent studies. The major objective of this review is to summarize the basic characteristics of biodegradable materials commonly used in indirect 3D bioprinting for vascularization, and to address recent advances in applying this technique to the vascularization of different tissues. Furthermore, the review describes how indirect 3D bioprinting creates blood vessels and vascularized tissue constructs by introducing the methodology and biodegradable ink selection. With the continuous improvement of biodegradable materials in the future, indirect 3D bioprinting will make further contributions to the development of this field.

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3D printing of functional nerve guide conduits

Cited by 22 publications

References 118 publications

Nerve transfer with 3D-printed branch nerve conduits

Nerve transfer with 3D-printed branch nerve conduits

Three Potential Elements of Developing Nerve Guidance Conduit for Peripheral Nerve Regeneration

Biodegradable Inks in Indirect Three-Dimensional Bioprinting for Tissue Vascularization

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