3D printing of ceramic scaffolds for engineering of bone tissue

Баринов, С. М.; Вахрушев, И. В.; Komlev, V. S.; Миронов, А. Н.; Попов, В. К.; Teterina, A. Yu.; Fedotov, A. Yu.; Yarygin, K. N.

doi:10.1134/s207511331504005x

Cited by 14 publications

(14 citation statements)

References 17 publications

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“…3D printed samples were made under a previously modified printing algorithm of ceramic constructions described elsewhere in details[ 12 ].…”

Section: Experimental Methodsmentioning

confidence: 99%

“…In this study, based on our previous experience in 3D printing of OCP bone substitutes[ 11 , 12 ], considering the critical role of angiogenesis for reparative osteogenesis, we hypothesized that deposition of plasmid DNA carrying a gene of vascular endothelial growth factor (pDNA- VEGFA ), as an active substance of the “Neovasculgen” drug (developed and certified for clinical applications by HSCI, Russia)[ 13 ], into custom-made OCP-based 3D printed implants would make it effective in large bone defect substitution and guided bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

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3D Printed Gene-activated Octacalcium Phosphate Implants for Large Bone Defects Engineering

Бозо

Деев

Smirnov

et al. 2024

IJB

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The aim of the study was the development of three-dimensional (3D) printed gene-activated implants based on octacalcium phosphate (OCP) and plasmid DNA encoding VEGFA. The first objective of the present work involved design and fabrication of gene-activated bone substitutes based on the OCP and plasmid DNA with VEGFА gene using 3D printing approach of ceramic constructs, providing the control of its architectonics compliance to the initial digital models. X-ray diffraction, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and compressive strength analyses were applied to investigate the chemical composition, microstructure, and mechanical properties of the experimental samples. The biodegradation rate and the efficacy of plasmid DNA delivery in vivo were assessed during standard tests with subcutaneous implantation to rodents in the next stage. The final part of the study involved substitution of segmental tibia and mandibular defects in adult pigs with 3D printed gene-activated implants. Biodegradation, osteointegration, and effectiveness of a reparative osteogenesis were evaluated with computerized tomography, SEM, and a histological examination. The combination of gene therapy and 3D printed implants manifested the significant clinical potential for effective bone regeneration in large/critical size defect cases.

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“…3D printed samples were made under a previously modified printing algorithm of ceramic constructions described elsewhere in details[ 12 ].…”

Section: Experimental Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

3D Printed Gene-activated Octacalcium Phosphate Implants for Large Bone Defects Engineering

Бозо

Деев

Smirnov

et al. 2024

IJB

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“…[85] Inkjet printing could achieve controllable and selective deposition of drug droplets onto microneedle surface by numerous thermal or piezoelectric-driven printing heads. [86] Currently, such technique has been main utilized to coat pre-fabricated microneedles with drugs for the personalized and combined drug loading. [87,88] Photopolymerization-based technique refers to the fabrication of 3D models by selectively polymerization of photo-sensitive polymers under the laser/light irradiation.…”

Section: Methods For the Fabrication Of Polymeric Microneedlesmentioning

confidence: 99%

Biomedical Applications of Polymeric Microneedles for Transdermal Therapeutic Delivery and Diagnosis: Current Status and Future Perspectives

Liu

Luo

Xing

2020

Advanced Therapeutics

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Transdermal drug delivery is a crucial extension of drug administration routes and has been widely acknowledged as an alternative way to traditional oral administration and subcutaneous injections due to the advantages such as increased dosage efficacy, decreased systemic side effect, and improved patient compliance. The past few decades have witnessed biomedical applications of microneedles in various cutting‐edge fields. Microneedles are needles with micron‐scale length which can pierce the epidermis of the skin in a minimally invasive manner for transdermal drug delivery. Compared with inorganic and metal microneedles, polymeric microneedles have attracted more attention because of their superior biocompatibility, nontoxicity, and biodegradability. In this review, the state‐of‐art and future biomedical applications of polymeric microneedles are summarized. First of all, a brief introduction to the polymeric microneedles, including types of polymeric microneedles and methods for the fabrication is included. Then the biomedical applications of polymeric microneedles in transdermal drug delivery and diagnosis are summarized in detail. Finally, discussions on the current limitations and future perspectives of polymeric microneedles are provided.

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“…A major limitation with this technique is that it uses hydrogels, which possess relatively weaker mechanical properties and therefore cannot withstand the mechanical load in vivo (Duan, Hockaday, Kang, & Butcher, 2013). Barinov et al (2015) printed several micro granules of tricalcium phosphate (TCP) and TCP-modified scaffolds using inkjet printing to develop bioactive ceramics for bone (Barinov et al, 2015). The printing process was performed using a multi-nozzle system with a droplet size of~40 pL and average diameter of the spot equivalent to 100 μm on the powder surface.…”

Section: Inkjet Bioprintingmentioning

confidence: 99%

Advances in three‐dimensional bioprinting of bone: Progress and challenges

Midha

Dalela

Sybil

et al. 2019

J Tissue Eng Regen Med

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Several attempts have been made to engineer a viable three‐dimensional (3D) bone tissue equivalent using conventional tissue engineering strategies, but with limited clinical success. Using 3D bioprinting technology, scientists have developed functional prototypes of clinically relevant and mechanically robust bone with a functional bone marrow. Although the field is in its infancy, it has shown immense potential in the field of bone tissue engineering by re‐establishing the 3D dynamic micro‐environment of the native bone. Inspite of their in vitro success, maintaining the viability and differentiation potential of such cell‐laden constructs overtime, and their subsequent preclinical testing in terms of stability, mechanical loading, immune responses, and osseointegrative potential still needs to be explored. Progress is slow due to several challenges such as but not limited to the choice of ink used for cell encapsulation, optimal cell source, bioprinting method suitable for replicating the heterogeneous tissues and organs, and so on. Here, we summarize the recent advancements in bioprinting of bone, their limitations, challenges, and strategies for future improvisations. The generated knowledge will provide deep insights on our current understanding of the cellular interactions with the hydrogel matrices and help to unravel new methodologies for facilitating precisely regulated stem cell behaviour.

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3D printing of ceramic scaffolds for engineering of bone tissue

Cited by 14 publications

References 17 publications

3D Printed Gene-activated Octacalcium Phosphate Implants for Large Bone Defects Engineering

3D Printed Gene-activated Octacalcium Phosphate Implants for Large Bone Defects Engineering

Biomedical Applications of Polymeric Microneedles for Transdermal Therapeutic Delivery and Diagnosis: Current Status and Future Perspectives

Advances in three‐dimensional bioprinting of bone: Progress and challenges

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