3D printing of a lithium-calcium-silicate crystal bioscaffold with dual bioactivities for osteochondral interface reconstruction

Chen, Lei; Deng, Chengbin; Li, Jiayi; Yao, Qingqiang; Chang, Jiang; Wang, Liming; Wu, Chengtie

doi:10.1016/j.biomaterials.2018.04.005

Cited by 187 publications

(111 citation statements)

References 66 publications

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“…The L2C4S4 bioceramic powders were synthesized and characterized as previously reported [10]. Pure TCP powders were prepared as the control.…”

Section: Preparation Of the Extracts Of L2c4s4 And Tcp Powdersmentioning

confidence: 99%

“…In order to solve these doubts, we synthesized a new L2C4S4 bioceramics which was proven to promote in vivo repair of osteochondral defects in our previous study [10]. In view of the advantageous stimulatory effects of the ionic products from L2C4S4 on chondrogenesis in vitro, we supposed that ionic products of this bioceramics may also biologically facilitate chondrogenic differentiation of iPSCs.…”

Section: Introductionmentioning

confidence: 99%

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A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy

Chen

Gao

et al. 2020

Stem Cell Res Ther

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Background: Induced pluripotent stem cells (iPSCs) exhibit limitless pluripotent plasticity and proliferation capability to provide an abundant cell source for tissue regenerative medicine. Thus, inducing iPSCs toward a specific differentiation direction is an important scientific question. Traditionally, iPSCs have been induced to chondrocytes with the help of some small molecules within 21-36 days. To speed up the differentiation of iPSCs, we supposed to utilize bioactive ceramics to assist chondrogenic-induction process. Methods: In this study, we applied ionic products (3.125~12.5 mg/mL) of the lithium-containing bioceramic (Li 2 Ca 4 Si 4 O 13 , L2C4S4) and individual Li + (5.78~23.73 mg/L) in the direct chondrogenic differentiation of human iPSCs. Results: Compared to pure chondrogenic medium and extracts of tricalcium phosphate (TCP), the extracts of L2C4S4 at a certain concentration range (3.125~12.5 mg/mL) significantly enhanced chondrogenic proteins Type II Collagen (COL II)/Aggrecan/ SRY-Box 9 (SOX9) synthesis and reduced hypertrophic protein type X collagen (COL X)/matrix metallopeptidase 13 (MMP13) production in iPSCs-derived chondrocytes within 14 days, suggesting that these newly generated chondrocytes exhibited favorable chondrocytes characteristics and maintained a low-hypertrophy state. Further studies demonstrated that the individual Li + ions at the concentration range of 5.78~23.73 mg/L also accelerated the chondrogenic differentiation of iPSCs, indicating that Li + ions played a pivotal role in chondrogenic differentiation process.Conclusions: These findings indicated that lithium-containing bioceramic with bioactive specific ionic components may be used for a promising platform for inducing iPSCs toward chondrogenic differentiation and cartilage regeneration.

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“…The L2C4S4 bioceramic powders were synthesized and characterized as previously reported [10]. Pure TCP powders were prepared as the control.…”

Section: Preparation Of the Extracts Of L2c4s4 And Tcp Powdersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy

Chen

Gao

et al. 2020

Stem Cell Res Ther

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“…The Mn-TCP scaffolds improve the regeneration of cartilage and subchondral bone tissues in rabbit osteochondral defects. 76 Chen et al 77 successfully synthesized a pure-phase lithium calcium silicate (Li 2 Ca 4 Si 4 O 13 , L2C4S4) bioceramic by a sol-gel method, and further constructed L2C4S4 scaffolds by a deposition manufacture technique at room temperature. The study demonstrated that L2C4S4 scaffold with high mechanical strength and dual bioactivities represent a promising biomaterial for osteochondral interface reconstruction.…”

Section: D Printing Of Articular Cartilagementioning

confidence: 99%

“…The study demonstrated that L2C4S4 scaffold with high mechanical strength and dual bioactivities represent a promising biomaterial for osteochondral interface reconstruction. 77 Zhang et al fabricated a multilayered osteochondral scaffold by LDM and thermal-induced phaseseparation techniques to repair goat articular cartilage defects. 78 Cohen et al proposed the concept of the in situ repair of cartilage and osteochondral defects using in situ AM technology 79 that expands the applications of 3D printing in cartilage tissue engineering.…”

Section: D Printing Of Articular Cartilagementioning

confidence: 99%

Three Dimensional Printing-Based Strategies for Functional Cartilage Regeneration

Shu

Chen

Guo

et al. 2019

Tissue Engineering Part B: Reviews

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The regeneration of cartilage has made great progress in the past few decades, Previous techniques for constructing tissue-engineered cartilage scaffolds mainly include particulate-leaching, gas-foaming, freeze-drying, and phase-separation techniques. Cartilage is heterogeneous, and it is difficult for traditional scaffolds to simulate such anisotropy. Therefore, the functional regeneration of cartilage is challenging. With advancements in additive manufacturing, it has become possible to prepare functional bionic scaffolds for structures and components by the codeposition of biological materials, cells, and active biomolecules, thereby achieving functional cartilage regeneration. This article reviews the applications of three dimensional (3D) printing techniques in the regeneration of cartilage at different anatomical locations, including articular cartilage, meniscus, intervertebral disc, and auricle. In addition, methods for preparing biomimetic constructs with regional structural gradients and regional componential gradients are discussed, with multinozzle 3D bioprinting technology as a future research direction for the functional regeneration and repair of cartilage.

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“…To address this issue, we studied the bone regeneration capacity mediated by pore sizes (100, 250, and 400 µm) of 3D printed β‐tricalcium phosphate (β‐TCP) scaffolds on two different bone defect models (calvarial and tibial). These pore sizes were chosen because a pore size range of 100–400 µm were considered good for bone regeneration and vascularization . Our goal is to identify the optimal pore size to effectively induce new bone formation in each model and understand the associated mechanism.…”

Section: Introductionmentioning

confidence: 99%

Bone Defect Model Dependent Optimal Pore Sizes of 3D‐Plotted Beta‐Tricalcium Phosphate Scaffolds for Bone Regeneration

et al. 2019

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Bones such as long bone and flat bone vary physiologically and anatomically. How pore sizes affect bone regeneration in different bone defects and its mechanisms are not well studied. Herein, typical long bone (tibia) defect model and flat bone (calvarial) defect model are applied to investigate their bone regeneration capacity and the underlying mechanism mediated by the pore sizes. Pore sizes (100, 250, and 400 µm) of the beta‐tricalcium phosphate (β‐TCP) scaffolds are strictly controlled utilizing a 3D plotting method. Bone regeneration capability in vivo by these pore sizes is related to the defect models (tibial defects and calvarial defects). Among the three pore sizes being studied, 100 µm pore size is most efficient in inducing bone formation in repairing flat bone defects, which is owing to the enhanced osteoblast differentiation in the intramembranous ossification. Differently, the scaffolds with a pore size of 400 µm display the best ability of bone formation for repairing long bone defects, which is speculated to be related to the accelerated formation of cartilage templates and ossification centers in endochondral ossification. This study suggests that bone defect type should be concerned in the design of the porous structure of an artificial material.

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3D printing of a lithium-calcium-silicate crystal bioscaffold with dual bioactivities for osteochondral interface reconstruction

Cited by 187 publications

References 66 publications

A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy

A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy

Three Dimensional Printing-Based Strategies for Functional Cartilage Regeneration

Bone Defect Model Dependent Optimal Pore Sizes of 3D‐Plotted Beta‐Tricalcium Phosphate Scaffolds for Bone Regeneration

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