3D printing in cell culture systems and medical applications

Lerman, Max J.; Lembong, Josephine; Gillen, Greg; Fisher, John P.

doi:10.1063/1.5046087

Cited by 46 publications

(19 citation statements)

References 138 publications

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“…This could represent a promising starting point for further investigations of intercellular interactions, or for assessing the effects of potential regulators on vessel formation. To the best knowledge of the authors, this experiment marks one of the first indirect co-cultivation assay in this vein that has been performed within a 3D-printed system—and thereby represents an important step towards the integration of customized 3D-printed devices in biomedical and/or tissue engineering applications [ 43 , 44 ].…”

Section: Resultsmentioning

confidence: 99%

“…Since the 3D printing material also enables phase contrast and fluorescence microscopy, the behaviour of cells could be observed over the entire cultivation period via both. To the best of the authors’ knowledge, there have been only few works published that demonstrates how 3D-printed systems can be used as customizable platforms in indirect 2D co-cultivation approaches [ 43 , 44 ]. We believe this paper therefore marks an important step forward for the integration of customized 3D-printed systems as self-contained test systems or equipment in biomedical applications.…”

Section: Discussionmentioning

confidence: 99%

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Customizable 3D-Printed (Co-)Cultivation Systems for In Vitro Study of Angiogenesis

et al. 2020

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Due to the ever-increasing resolution of 3D printing technology, additive manufacturing is now even used to produce complex devices for laboratory applications. Personalized experimental devices or entire cultivation systems of almost unlimited complexity can potentially be manufactured within hours from start to finish—an enormous potential for experimental parallelization in a highly controllable environment. This study presents customized 3D-printed co-cultivation systems, which qualify for angiogenesis studies. In these systems, endothelial and mesenchymal stem cells (AD-MSC) were indirectly co-cultivated—that is, both cell types were physically separated through a rigid, 3D-printed barrier in the middle, while still sharing the same cell culture medium that allows for the exchange of signalling molecules. Biochemical-based cytotoxicity assays initially confirmed that the 3D printing material does not exert any negative effects on cells. Since the material also enables phase contrast and fluorescence microscopy, the behaviour of cells could be observed over the entire cultivation via both. Microscopic observations and subsequent quantitative analysis revealed that endothelial cells form tubular-like structures as angiogenic feature when indirectly co-cultured alongside AD-MSCs in the 3D-printed co-cultivation system. In addition, further 3D-printed devices are also introduced that address different issues and aspire to help in varying experimental setups. Our results mark an important step forward for the integration of customized 3D-printed systems as self-contained test systems or equipment in biomedical applications.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Customizable 3D-Printed (Co-)Cultivation Systems for In Vitro Study of Angiogenesis

et al. 2020

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“…3D-printing is a rapidly evolving technology that provides an opportunity to fabricate complex 3D structures for biological applications[ 5 , 27 ]. It is an important tool for translational research that focuses on the in vitro biology and disease models in bioreactors.…”

Section: 3d-printed Bioreactor For Biological Applicationsmentioning

confidence: 99%

3D-printed Bioreactors for In Vitro Modeling and Analysis

Priyadarshini¹,

Dikshit²,

Zhang³

2024

IJB

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In recent years, three-dimensional (3D) printing has markedly enhanced the functionality of bioreactors by offering the capability of manufacturing intricate architectures, which changes the way of conducting in vitro biomodeling and bioanalysis. As 3D-printing technologies become increasingly mature, the architecture of 3D-printed bioreactors can be tailored to specific applications using different printing approaches to create an optimal environment for bioreactions. Multiple functional components have been combined into a single bioreactor fabricated by 3D-printing, and this fully functional integrated bioreactor outperforms traditional methods. Notably, several 3D-printed bioreactors systems have demonstrated improved performance in tissue engineering and drug screening due to their 3D cell culture microenvironment with precise spatial control and biological compatibility. Moreover, many microbial bioreactors have also been proposed to address the problems concerning pathogen detection, biofouling, and diagnosis of infectious diseases. This review offers a reasonably comprehensive review of 3D-printed bioreactors for in vitro biological applications. We compare the functions of bioreactors fabricated by various 3D-printing modalities and highlight the benefit of 3D-printed bioreactors compared to traditional methods.

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“…Three-dimensional (3D) tissue-specific cultures have been a powerful tool in disease therapy in recent years, and a large number of studies have been conducted on various diseases [ 203 ]. Currently, 3D structures can be derived from pluripotent stem cells (including ESCs and iPSCs) or adult stem cells (including epithelial cells and MSCs [ 204 , 205 ].…”

Section: Future Perspectives Of Mensc-derived Small Evs In Regenerative Medicinementioning

confidence: 99%

Small extracellular vesicles from menstrual blood-derived mesenchymal stem cells (MenSCs) as a novel therapeutic impetus in regenerative medicine

Chen

Mei

et al. 2021

Stem Cell Res Ther

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Menstrual blood-derived mesenchymal stem cells (MenSCs) have great potential in regenerative medicine. MenSC has received increasing attention owing to its impressive therapeutic effects in both preclinical and clinical trials. However, the study of MenSC-derived small extracellular vesicles (EVs) is still in its initial stages, in contrast to some common MSC sources (e.g., bone marrow, umbilical cord, and adipose tissue). We describe the basic characteristics and biological functions of MenSC-derived small EVs. We also demonstrate the therapeutic potential of small EVs in fulminant hepatic failure, myocardial infarction, pulmonary fibrosis, prostate cancer, cutaneous wound, type-1 diabetes mellitus, aged fertility, and potential diseases. Subsequently, novel hotspots with respect to MenSC EV-based therapy are proposed to overcome current challenges. While complexities regarding the therapeutic potential of MenSC EVs continue to be unraveled, advances are rapidly emerging in both basic science and clinical medicine. MenSC EV-based treatment has great potential for treating a series of diseases as a novel therapeutic strategy in regenerative medicine.

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3D printing in cell culture systems and medical applications

Cited by 46 publications

References 138 publications

Customizable 3D-Printed (Co-)Cultivation Systems for In Vitro Study of Angiogenesis

Customizable 3D-Printed (Co-)Cultivation Systems for In Vitro Study of Angiogenesis

3D-printed Bioreactors for In Vitro Modeling and Analysis

Small extracellular vesicles from menstrual blood-derived mesenchymal stem cells (MenSCs) as a novel therapeutic impetus in regenerative medicine

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