3D Printing Hydrogel Scaffolds with Nanohydroxyapatite Gradient to Effectively Repair Osteochondral Defects in Rats

Zhang, Hui; Huang, Hongtao; Hao, Guangrun; Zhang, Yongsheng; Ding, Hao; Fan, Zengjie; Sun, Luyi

doi:10.1002/adfm.202006697

Cited by 94 publications

(109 citation statements)

References 49 publications

(32 reference statements)

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“…The recapitulation of the mechanical properties of cartilage is a highly challenging task because cartilage is a load-bearing tissue that is exposed to continuous and repeated friction and compression. The use of bioprinting approaches that employ multi-materials[ 33 , 34 ], multi-cell types[ 31 ], and multi-stages[ 34 , 35 ] has enabled the substantial progress in this particular front. Today, relatively complex and large (~ 1–10 cm 3 ) bioprinted constructs of cartilage have been implanted in large animal models[ 33 , 35 ] with excellent results in terms of both integration and mechanical performance.…”

Section: Applicationsmentioning

confidence: 99%

Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

Pedroza-González¹,

Rodríguez-Salvador²,

Pérez-Benítez³

et al. 2024

IJB

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This scientometric analysis of 393 original papers published from January 2000 to June 2019 describes the development and use of bioinks for 3D bioprinting. The main trends for bioink applications and the primary considerations guiding the selection and design of current bioink components (i.e., cell types, hydrogels, and additives) were reviewed. The cost, availability, practicality, and basic biological considerations (e.g., cytocompatibility and cell attachment) are the most popular parameters guiding bioink use and development. Today, extrusion bioprinting is the most widely used bioprinting technique. The most reported use of bioinks is the generic characterization of bioink formulations or bioprinting technologies (32%), followed by cartilage bioprinting applications (16%). Similarly, the cell-type choice is mostly generic, as cells are typically used as models to assess bioink formulations or new bioprinting methodologies rather than to fabricate specific tissues. The cell-binding motif arginine-glycine-aspartate is the most common bioink additive. Many articles reported the development of advanced functional bioinks for specific biomedical applications; however, most bioinks remain the basic compositions that meet the simple criteria: Manufacturability and essential biological performance. Alginate and gelatin methacryloyl are the most popular hydrogels that meet these criteria. Our analysis suggests that present-day bioinks still represent a stage of emergence of bioprinting technology.

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Section: Applicationsmentioning

confidence: 99%

Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

Pedroza-González¹,

Rodríguez-Salvador²,

Pérez-Benítez³

et al. 2024

IJB

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“…After implantation at the defect site, osseointegration to the scaffold readily occurs due to bone's high metabolism and cell content, including stem cells [ 10 ]. Because of the scaffold's intricate stratified structure, the vertical integration of cartilage to the underlying bone occurs to a considerable extent [ 11 , 12 ]. Nevertheless, the lateral integration of neocartilage to adjacent cartilage is a difficult problem that urgently needs to be solved [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Marginal sealing around integral bilayer scaffolds for repairing osteochondral defects based on photocurable silk hydrogels

Zhou

Jiang

et al. 2021

Bioactive Materials

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Osteochondral repair remains a major challenge in current clinical practice despite significant advances in tissue engineering. In particular, the lateral integration of neocartilage into surrounding native cartilage is a difficult and inadequately addressed problem that determines the success of tissue repair. Here, a novel design of an integral bilayer scaffold combined with a photocurable silk sealant for osteochondral repair is reported. First, we fabricated a bilayer silk scaffold with a cartilage layer resembling native cartilage in surface morphology and mechanical strength and a BMP-2-loaded porous subchondral bone layer that facilitated the osteogenic differentiation of BMSCs. Second, a TGF-β3-loaded methacrylated silk fibroin sealant (Sil-MA) exhibiting biocompatibility and good adhesive properties was developed and confirmed to promote chondrocyte migration and differentiation. Importantly, this TGF-β3-loaded Sil-MA hydrogel provided a bridge between the cartilage layer of the scaffold and the surrounding cartilage and then guided new cartilage to grow towards and replace the degraded cartilage layer from the surrounding native cartilage in the early stage of knee repair. Thus, osteochondral regeneration and superior lateral integration were achieved in vivo by using this composite. These results demonstrate that the new approach of marginal sealing around the cartilage layer of bilayer scaffolds with Sil-MA hydrogel has tremendous potential for clinical use in osteochondral regeneration.

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“…The recent development of advanced biomaterials including inorganic materials [ [15] , [16] , [17] ], organic polymers [ [18] , [19] , [20] , [21] , [22] , [23] , [24] ], and their composites [ 25 , 26 ] has shown great promise for accelerating bone reconstruction [ 27 , 28 ], providing alternatives bone remodeling methods [ [29] , [30] , [31] , [32] ]. Despite the outcomes of biomaterials for bone repair, many issues still hinder the clinical transformation from several aspects.…”

Section: Introductionmentioning

confidence: 99%

Implantable blood clot loaded with BMP-2 for regulation of osteoimmunology and enhancement of bone repair

Fan

Bai

Shan

et al. 2021

Bioactive Materials

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The treatment of large-area bone defects still faces many difficulties and challenges. Here, we developed a blood clot delivery platform loaded with BMP-2 protein (BMP-2@BC) for enhanced bone regeneration. Blood clot gel platform as natural biomaterials can be engineered from autologous blood. Once implanted into the large bone defect site, it can be used for BMP-2 local delivery, as well as modulating osteoimmunology by recruiting a great number of macrophages and regulating their polarization at different stages. Moreover, due to the deep-red color of blood clot gel, mild localized hyperthermia under laser irradiation further accelerated bone repair and regeneration. We find that the immune niche within the bone defect microenvironment can be modulated in a controllable manner by the blood clots implantation and laser treatment. We further demonstrate that the newly formed bone covered almost 95% of the skull defect area by our strategy in both mice and rat disease models. Due to the great biocompatibility, photothermal potential, and osteoimmunomodulation capacity, such technology shows great promise to be used in further clinical translation.

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3D Printing Hydrogel Scaffolds with Nanohydroxyapatite Gradient to Effectively Repair Osteochondral Defects in Rats

Cited by 94 publications

References 49 publications

Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

Marginal sealing around integral bilayer scaffolds for repairing osteochondral defects based on photocurable silk hydrogels

Implantable blood clot loaded with BMP-2 for regulation of osteoimmunology and enhancement of bone repair

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