“…A variety of biomaterials (i.e., bioinks) have been used for tissue bioprinting, including ceramics, synthetic and natural polymers, decellularized tissues, and more frequently, hybrid bioinks consisting of a combination of these materials [8][9][10][11].While significant and rapid progresses have been made in tissue bioprinting processes for various in vitro applications, such as disease modeling [12] and drug screening [13], there are several challenges to address before bioprinting becomes clinically relevant [14][15][16]. These constraints include: 1) limited number of available bioink solutions and lack of thorough characterization of their biological and physiomechanical properties [10,17]; 2) poor understanding of the correlation between printed architecture and the ultimate tissue function [18,19]; 3) limitations on the quality of imaging techniques [20,21] and available bioprinters [22]; 4) complex and rather expensive processes involved pre, during, and post-bioprinting [22]; 5) suboptimal, non-specialized printing software and their often incompatibilities [23].There are eight articles published in this Special Issue composed of four research papers and four review papers. The research articles focus on the influence of electron beam (E-beam) sterilization on in vivo degradation of composite filaments [24], enhancing osteogenic differentiation of stem cells using 3D printed wavy scaffolds [25], the development of a scaffold-free bioprinter [26], and the fabrication of multilayered vascular constructs with a curved structure and multi-branches [27].…”