3D-Printed Replica and Porcine Explants for Pre-Clinical Optimization of Endoscopic Tumor Treatment by Magnetic Targeting

Roeth, Anjali A.; Garretson, Ian C.; Beltz, Maja; Herbold, Till; Schulze-Hagen, Maximilian; Quaisser, Sebastian; Georgens, Alex; Reith, Dirk; Slabu, Ioana; Klink, Christian D.; Neumann, Ulf; Linke, Barbara

doi:10.3390/cancers13215496

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…This is because the tumor cells in 3D tumor model aggregated in clusters to form tumor spheres thereby reducing its sensitivity to drugs and increasing drug resistance. It has been shown that in 2D culture, only a planar structure existed without kinetic factors, while MDA-MB-231 cells were cultured in 2D monolayer flat, where the cells on the surface could be directly exposed to the drug and thus could directly influence the cell state [75,76].…”

Section: Cytotoxicity Test Of Curnps In 3d Tumor Model/environmentmentioning

confidence: 99%

Curcumin nanoparticles combined with 3D printed bionic tumor models for breast cancer treatment

Su¹,

Hu²,

Kang³

et al. 2022

Biofabrication

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Compared with conventional therapeutic approaches, nanomedicines are attracting a growing interest due to their better targeting ability, higher delivery efficiency, and good water solubility. However, conventional drug efficacy assessment methods are based on a two-dimensional (2D) culture approach of single cells to obtain in vitro therapeutic effects, which may not be representative of actual tumors. Based on the above considerations, the three-dimensional (3D) cell culture models became a better choice since they can increase the complexity of in vitro systems and provide a biomimetic microenvironment that is closer to the in vivo native than two-dimensional (2D) cultures. In our study, Chinese medicine nanoparticles (CurNPs) with good water solubility and good tumor therapeutic effects were prepared by combining polymeric non-ionic surfactant (Pluronic F127) with curcumin. The hybrid scaffolds based on nano-clay, sodium alginate, and gelatin were also prepared, which showed good printability and excellent biocompatibility. We then studied the therapeutic effects of CurNPs on metastatic breast cancer using a 3D tumor model fabricated with scaffold-bound metastatic breast cancer (MDA-MB-231) cells. It was showed that the 3D cell model presented better cell proliferation effect while compared with 2D version. Additionally, there was good enhanced permeability and retention effect (EPR) effect when CurNPs entered with better accumulate in 3D cell “tumor” sites which represented more realistic response of a more real tumor treatment effect for breast cancer cells. Our study indicated that the combinational of nanomaterials with 3D cell ‘tumor’ models provided an alternative and better platform for drug screening and has great potential be used as safe and effective treatment screening for breast cancer.

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Section: Cytotoxicity Test Of Curnps In 3d Tumor Model/environmentmentioning

confidence: 99%

Curcumin nanoparticles combined with 3D printed bionic tumor models for breast cancer treatment

Su¹,

Hu²,

Kang³

et al. 2022

Biofabrication

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“…Animal models deliver the necessary tissue function but also have limitations (i.e., the comparatively small number of specimens that can be investigated and high variability in the experimental conditions, e.g., due to biodynamic signatures) [14]. In addition, it is recommended to avoid animal experiments whenever possible [15]; however, reliable alternatives exist only for macroscopic structures [16] that do not mimic human tissue function. In tissue engineering, 3D models of vascular networks are already used for the design of scaffolds [17].…”

Section: Introductionmentioning

confidence: 99%

Towards Realistic 3D Models of Tumor Vascular Networks

Lindemann,

Glänzer,

Roeth

et al. 2023

Cancers

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For reliable in silico or in vitro investigations in, for example, biosensing and drug delivery applications, accurate models of tumor vascular networks down to the capillary size are essential. Compared to images acquired with conventional medical imaging techniques, digitalized histological tumor slices have a higher resolution, enabling the delineation of capillaries. Volume rendering procedures can then be used to generate a 3D model. However, the preparation of such slices leads to misalignments in relative slice orientation between consecutive slices. Thus, image registration algorithms are necessary to re-align the slices. Here, we present an algorithm for the registration and reconstruction of a vascular network from histologic slices applied to 169 tumor slices. The registration includes two steps. First, consecutive images are incrementally pre-aligned using feature- and area-based transformations. Second, using the previous transformations, parallel registration for all images is enabled. Combining intensity- and color-based thresholds along with heuristic analysis, vascular structures are segmented. A 3D interpolation technique is used for volume rendering. This results in a 3D vascular network with approximately 400–450 vessels with diameters down to 25–30 µm. A delineation of vessel structures with close distance was limited in areas of high structural density. Improvement can be achieved by using images with higher resolution and or machine learning techniques.

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“…In recent years, computer-aided surgery (CAS) in the field of oral and maxillofacial treatment and the virtual planning of craniofacial surgical procedures are becoming increasingly popular [ 1 , 2 , 3 ]. Individualized 3D-printed templates and models, as an important computer-assisted surgery technology, is often applied for preoperative simulation, osteotomy, and occlusal reconstruction [ 2 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Clinical Application of Individualized 3D-Printed Templates in the Treatment of Condylar Osteochondroma

Niu

Wang

et al. 2022

Healthcare

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Background: Osteochondroma (OC) is one of the most common benign tumors of the long bones, but it rarely occurs in the maxillofacial skeleton. However, mandibular condylar OC often leads to severe facial deformity in affected patients, including facial asymmetry, deviation of the chin, and malocclusion. This study aimed to explore the clinical application of individualized 3D-printed templates to accurately and effectively treat condylar OC. Methods: A total of 8 patients with mandibular condylar OC were treated from July 2015 to August 2021. The enrolled patients (5 women and 3 men) had a median age of 27 years (range: 21–32 years). All patients exhibited symptoms of facial asymmetry and occlusal disorders preoperatively. The digital software used to virtually design the process consisted of three-dimensional reconstruction, 3D-cephalometry analysis, virtual surgery, individualized templates, and postoperative facial soft-tissue prediction. A set of 3D-printed templates (DOS and DOT) were used in all cases to stabilize the occlusion and guide the osteotomy. Then, pre- and post-operative complications, mouth opening, clinical signs, and the accuracy of the CT imaging analysis were all evaluated. All the measurement data were presented as means ± SD; Bonferroni and Tamhane T2 multiple comparison tests were used to examine the differences between the groups. Results: All patients healed uneventfully. None of the patients exhibited facial nerve injury at follow-up. In comparing the condylar segments with T0p and T1, the average deviation of the condylar segments was 0.5796 mm, indicating that the post-operative reconstructed condyles showed a high degree of similarity to the reconstruction results of the virtual surgical plan. Conclusions: Individualized 3D-printed templates simplified surgical procedures and improved surgical accuracy, proving to be an effective method for the treatment of patients with slight asymmetric deformities secondary to condylar OC.

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3D-Printed Replica and Porcine Explants for Pre-Clinical Optimization of Endoscopic Tumor Treatment by Magnetic Targeting

Cited by 4 publications

References 33 publications

Curcumin nanoparticles combined with 3D printed bionic tumor models for breast cancer treatment

Curcumin nanoparticles combined with 3D printed bionic tumor models for breast cancer treatment

Towards Realistic 3D Models of Tumor Vascular Networks

Clinical Application of Individualized 3D-Printed Templates in the Treatment of Condylar Osteochondroma

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