3D printed porous biomimetic composition sustained release zoledronate to promote osteointegration of osteoporotic defects

Bai, Haotian; Cui, Yutao; Wang, Chenyu; Wang, Zhonghan; Luo, Wenbin; Liu, Yuzhe; Leng, Yangming; Wang, Jincheng; Li, Zuhao; Li, He

doi:10.1016/j.matdes.2020.108513

Cited by 60 publications

(41 citation statements)

References 50 publications

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“…In such cases, the size of the scaffold had to be limited to ensure sufficient oxygenation and nutrition to avoid transplant failure. VEGF is the factor dominantly involved in angiogenesis, as it is a potent inducer of endothelial cell migration and proliferation [ 48 ]. With the results above, it can be seen that our novel bi-layer scaffold has the potential to induce osteogenesis, cementogenesis, and angiogenesis.…”

Section: Resultsmentioning

confidence: 99%

Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration

et al. 2021

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Periodontal disease is a chronic disease that can lead to lose teeth and even tooth loss if left untreated. Osteoporosis and periodontal disease share similar characteristics and associated factors. Current regenerative techniques for periodontal diseases are ineffective in restoring complete function and structural integrity of periodontium due to unwanted migration of cells. In this study, we applied the concept of guided tissue regeneration (GTR) and 3D fabricated gingival fibroblast cell-laden collagen/strontium-doped calcium silicate (SrCS) bi-layer scaffold for periodontal regeneration. The results revealed that the bioactive SrCS had a hydroxyapatite formation on its surface after 14 days of immersion and that SrCS could release Sr and Si ions even after 6 months of immersion. In addition, in vitro results showed that the bi-layer scaffold enhanced secretion of FGF-2, BMP-2, and VEGF from human gingival fibroblasts and increased secretion of osteogenic-related proteins ALP, BSP, and OC from WJMSCs. In vivo studies using animal osteoporotic models showed that the 3D-printed cell-laden collagen/SrCS bi-layer scaffold was able to enhance osteoporotic bone regeneration, as seen from the increased Tb.Th and BV/TV ratio and the histological stains. In conclusion, it can be seen that the bi-layer scaffolds enhanced osteogenesis and further showed that guided periodontal regeneration could be achieved using collagen/SrCS scaffolds, thus making it a potential candidate for future clinical applications.

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Section: Resultsmentioning

confidence: 99%

Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration

et al. 2021

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“…Osteogenic differentiation is characterized by calcium deposits, which was indicated by AR staining. 34 As demonstrated in Figure 5(a) , BMSCs cultured with hydrogel incorporated pTi scaffolds (pTi@L-Gel and pTi@M-Gel groups) were strongly stained by AR, especially after 21 days of incubation. However, this mineralization rate slowed down in pTi@H-Gel group at 21 days, compared with pTi@L-Gel and pTi@M-Gel groups.…”

Section: Resultsmentioning

confidence: 90%

“…3D printed Ti 6 Al 4 V porous scaffolds were prepared by additive manufacturing technique via an EBM system (Q10, Arcam, Sweden) as previously reported. 34 Spherical titanium alloy powder (Grade 23, particle size 45–100 μm) was melted layer by layer according to the preset parameters. The pTi scaffolds were designed based on about 70% in porosity and about 600 μm in pore diameter.…”

Section: Methodsmentioning

confidence: 99%

The combination of multi-functional ingredients-loaded hydrogels and three-dimensional printed porous titanium alloys for infective bone defect treatment

Qiao

et al. 2020

J Tissue Eng

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Biomaterial with the dual-functions of bone regeneration and antibacterial is a novel therapy for infective bone defects. Three-dimensional (3D)-printed porous titanium (pTi) benefits bone ingrowth, but its microporous structure conducive to bacteria reproduction. Herein, a multifunctional hydrogel was prepared from dynamic supramolecular assembly of sodium tetraborate (Na2B4O7), polyvinyl alcohol (PVA), silver nanoparticles (AgNPs) and tetraethyl orthosilicate (TEOS), and composited with pTi as an implant system. The pTi scaffolds have ideal pore size and porosity matching with bone, while the supramolecular hydrogel endows pTi scaffolds with antibacterial and biological activity. In vitro assessments indicated the 3D composite implant was biocompatible, promoted bone marrow mesenchymal stem cells (BMSCs) proliferation and osteogenic differentiation, and inhibited bacteria, simultaneously. In vivo experiments further demonstrated that the implant showed effective antibacterial ability while promoting bone regeneration. Besides distal femur defect, the innovative scaffolds may also serve as an ideal biomaterial (e.g. dental implants) for other contaminated defects.

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“…Therefore, improving the bioactivity of osteoblast-related cells on the surface of titanium alloy is anticipated to treat the postoperative complications of osteoporosis patients. Bai et al ( 17 ) constructed a pTi/poloxamer 407 hydrogel system loaded with zoledronate, a bisphosphonate, thus obtaining an optimized osseointegration effect in osteoporotic defect models. In addition, Vladescu et al ( 10 ) coated 3D-printed Ti6Al4V scaffolds with HA, and calcium phosphate nanoparticles to improve the bioactivity and biocompatibility of the scaffolds.…”

Section: Introductionmentioning

confidence: 99%

3D‑printed Ti6Al4V scaffolds combined with pulse electromagnetic fields enhance osseointegration in osteoporosis

Ye¹,

Liu²,

Yan³

et al. 2021

Mol Med Rep

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The loosening and displacement of prostheses after dental implantation and arthroplasty is a substantial medical burden due to the complex correction surgery. Three-dimensional (3D)-printed porous titanium (pTi) alloy scaffolds are characterized by low stiffness, are beneficial to bone ingrowth, and may be used in orthopedic applications. However, for the bio-inert nature between host bone and implants, titanium alloy remains poorly compatible with osseointegration, especially in disease conditions, such as osteoporosis. In the present study, 3D-printed pTi scaffolds with ideal pore size and porosity matching the bone tissue, were combined with pulse electromagnetic fields (PEMF), an exogenous osteogenic induction stimulation, to evaluate osseointegration in osteoporosis. In vitro, external PEMF significantly improved osteoporosis-derived bone marrow mesenchymal stem cell proliferation and osteogenic differentiation on the surface of pTi scaffolds by enhancing the expression of alkaline phosphatase, runt-related transcription factor-2, osteocalcin, and bone morphogenetic protein-2. In vivo, Microcomputed tomography analysis and histological evaluation indicated the external PEMF markedly enhanced bone regeneration and osseointegration. This novel therapeutic strategy has potential to promote osseointegration of dental implants or artificial prostheses for patients with osteoporosis.

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3D printed porous biomimetic composition sustained release zoledronate to promote osteointegration of osteoporotic defects

Cited by 60 publications

References 50 publications

Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration

Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration

The combination of multi-functional ingredients-loaded hydrogels and three-dimensional printed porous titanium alloys for infective bone defect treatment

3D‑printed Ti6Al4V scaffolds combined with pulse electromagnetic fields enhance osseointegration in osteoporosis

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