3D-Printed Poly(ε-Caprolactone)/Hydroxyapatite Scaffolds Modified with Alkaline Hydrolysis Enhance Osteogenesis In Vitro

Park, Sangbae; Kim, Jae Eun; Han, Jinsub; Jeong, Seung; Lim, Jae Woon; Lee, Myung Chul; Son, Hyunmok; Kim, Hong Bae; Choung, Yun‐Hoon; Seonwoo, Hoon; Chung, Jong Hoon; Jang, Kitae

doi:10.3390/polym13020257

Cited by 32 publications

(20 citation statements)

References 36 publications

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“…A previous work compared the differentiation potential of PCL and PCL/HA scaffolds with hDPCs, human umbilical cord mesenchymal stem cells (MSCs) and human bone marrow derived MSCs, with the hDPSCs presenting superior osteogenic outcomes [41]. Other have been successfully applying hDPSCs for the evaluation of PCL/HA scaffolds for bone tissue regeneration [46,52,53].…”

Section: Discussionmentioning

confidence: 99%

3D Printed Poly(?-Caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on Composite Preparation by Melt Blending or Solvent Casting Techniques and Influence of Bioceramic Content on Scaffold Properties

Biscaia¹,

Branquinho²,

Alvites³

et al. 2022

Preprint

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Bone tissue engineering has been developed in the past decades, with the engineering of bone substitutes on the vanguard of this regenerative approach. PCL based scaffolds are fairly applied for bone regeneration, and several composites have been incorporated, as to improve the devices’ mechanical properties and tissue ingrowth. In this study, HA was incorporated on PCL based scaffolds with two different proportions, 80:20 and 60:40. Devices were produced with two different techniques, SC and MB, and further investigated with regards to their mechanical characteristics and in vitro cytocompatibility. Results show the MB devices to present more promising mechanical properties, along with the incorporation of HA. The latter is also related to an increase in osteogenic activity and promotion. Overall, this study suggest PCL:HA scaffolds to be promising candidates for bone tissue engineering, particularly when produced by the MB method.

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Section: Discussionmentioning

confidence: 99%

3D Printed Poly(?-Caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on Composite Preparation by Melt Blending or Solvent Casting Techniques and Influence of Bioceramic Content on Scaffold Properties

Biscaia¹,

Branquinho²,

Alvites³

et al. 2022

Preprint

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“…Specific biomaterials such as those mentioned above and living cells become the ink needed to print scaffolds and structures on which cells are seeded. This technology is also applied to dentistry and a recent study demonstrated that DPSCs grown on a scaffold obtained with different biomaterials and printed with the 3D bioprinter show high cell viability and osteogenic differentiation as well as mineralization comparable to more canonical cell culture [ 107 – 109 ].…”

Section: Overview Of Different Types Of Dental Stem Cellsmentioning

confidence: 99%

Hypes and Hopes of Stem Cell Therapies in Dentistry: a Review

Baena

Casasco

Monti

2022

Stem Cell Rev and Rep

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One of the most exciting advances in life science research is the development of 3D cell culture systems to obtain complex structures called organoids and spheroids. These 3D cultures closely mimic in vivo conditions, where cells can grow and interact with their surroundings. This allows us to better study the spatio-temporal dynamics of organogenesis and organ function. Furthermore, physiologically relevant organoids cultures can be used for basic research, medical research, and drug discovery. Although most of the research thus far focuses on the development of heart, liver, kidney, and brain organoids, to name a few, most recently, these structures were obtained using dental stem cells to study in vitro tooth regeneration. This review aims to present the most up-to-date research showing how dental stem cells can be grown on specific biomaterials to induce their differentiation in 3D. The possibility of combining engineering and biology principles to replicate and/or increase tissue function has been an emerging and exciting field in medicine. The use of this methodology in dentistry has already yielded many interesting results paving the way for the improvement of dental care and successful therapies. Graphical abstract

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“…All subjects were undergoing extractions as a part of routine orthodontic or periodontal therapy. The sample size determination was conducted based on previous studies that have performed similar studies on stem cells [13,14]. However, sample size is not a critical determinant in stem cell experiments as stem cells have uniform properties and are cultured through many passages for the tests.…”

Section: Sample Size Determination and Sample Collectionmentioning

confidence: 99%

Preliminary Evaluation of Proliferation, Wound Healing Properties, Osteogenic and Chondrogenic Potential of Dental Pulp Stem Cells Obtained from Healthy and Periodontitis Affected Teeth

Fageeh

2021

Cells

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Background: Dental pulp tissue within the central cavity of the tooth is composed of dental pulp stem cells (DPSC). These mesenchymal stem cells have good proliferative as well as differentiation potential. DPSC has been isolated even from teeth with inflamed pulps and is found to retain their proliferative and differentiation potential. Little research is available about the viability and differentiation potential of DPSC obtained from teeth with periodontitis. In the present study, the aim was to compare the morphological features, stem cell marker (MSC) expression, proliferation rate, migratory and wound healing properties, osteogenic and chondrogenic differentiation potential of DPSCs obtained from periodontally healthy teeth (hDPSCs) and periodontitis affected teeth (pDPSCs). Methods: Dental pulp tissue was obtained from periodontally healthy volunteers (n = 3) and patients with periodontitis undergoing extraction of mobile teeth (n = 3). DPSC were isolated using the explant technique and cultured. All the experiments were performed at early passage (Passage 2), late passage (Passage 6) and after cryopreservation. Morphological features of the hDPSCs and pDPSCs were ascertained using microscopy. The expression of cell surface stem cell markers was assessed by the flow cytometry method. The proliferation and growth rate of the cells were assayed by plotting a growth curve from 0–13 days of culture. The migratory characteristics were assessed by wound scratch assay. Osteogenic and chondrogenic differentiation of the cells was assessed using standard protocols with and without induction. Results: DPSCs were successfully obtained from periodontally healthy teeth (hDPSC) and periodontitis-affected teeth (pDPSCs). The data suggests that there were no morphological differences observed in early passage cells between the two cohorts. Cryopreservation did change the morphology of pDSPCs. There was no significant difference in the positive expression of mesenchymal markers CD73, CD90 and CD105 in early passage cells. However, serial passaging and cryopreservation affected the marker expression in pDPSCs. A faint expression of hematopoietic stem cell markers CD34, CD45 and MHC class II antigen HLA-DR was observed in both the cell types. The expression of HLA-DR is upregulated in pDPSCs compared to hDPSC. A significantly slower growth rate and slower wound healing properties was observed in pDPSCs compared to hDPSC. In late passage and after cryopreservation, the migratory ability of pDPSCs was found to be increased drastically. There was no significant difference in osteogenic potential between the two cell types. However, the chondrogenic potential of pDPSCs was significantly lower compared to hDPSc. Yet, pDPSCs showed enhanced osteogenesis and chondrogenesis at late passage as well as after cryopreservation. Conclusion: The results of this novel study shed light on the isolation of viable DPSC from periodontitis-affected teeth. These cells exhibit a slower growth rate and migratory characteristics compared to their healthy counterparts. There was no difference in osteogenic potential but a reduction in chondrogenic potential was seen in pDPSCs compared to hDPSC. The findings reveal that DPSC from periodontitis-affected teeth presents an easy and viable option for regenerative medicine application. Some additional nutritive factors and protocols may be required to attain better regenerative benefits while using pDPSCs.

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3D-Printed Poly(ε-Caprolactone)/Hydroxyapatite Scaffolds Modified with Alkaline Hydrolysis Enhance Osteogenesis In Vitro

Cited by 32 publications

References 36 publications

3D Printed Poly(?-Caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on Composite Preparation by Melt Blending or Solvent Casting Techniques and Influence of Bioceramic Content on Scaffold Properties

3D Printed Poly(?-Caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on Composite Preparation by Melt Blending or Solvent Casting Techniques and Influence of Bioceramic Content on Scaffold Properties

Hypes and Hopes of Stem Cell Therapies in Dentistry: a Review

Preliminary Evaluation of Proliferation, Wound Healing Properties, Osteogenic and Chondrogenic Potential of Dental Pulp Stem Cells Obtained from Healthy and Periodontitis Affected Teeth

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