3D-Printed Poly-Caprolactone Scaffolds Modified With Biomimetic Extracellular Matrices for Tarsal Plate Tissue Engineering

Abstract

Tarsal plate regeneration has always been a challenge in the treatment of eyelid defects. The commonly used clinical treatments such as hard palate mucosa grafts cannot achieve satisfactory repair effects. Tissue engineering has been considered as a promising technology. However, tarsal plate tissue engineering is difficult to achieve due to its complex structure and lipid secretion function. Three-dimensional (3D) printing technology has played a revolutionary role in tissue engineering because it can fabricate complex scaffolds through computer aided design (CAD). In this study, it was novel in applying 3D printing technology to the fabrication of tarsal plate scaffolds using poly-caprolactone (PCL). The decellularized matrix of adipose-derived mesenchymal stromal cells (DMA) was coated on the surface of the scaffold, and its biofunction was further studied. Immortalized human SZ95 sebocytes were seeded on the scaffolds so that neutral lipids were secreted for replacing meibocytes. In vitro experiments revealed excellent biocompatibility of DMA-PCL scaffolds with sebocytes. In vivo experiments revealed excellent sebocytes proliferation on the DMA-PCL scaffolds. Meanwhile, sebocytes seeded on the scaffolds secreted abundant neutral lipid in vitro and in vivo. In conclusion, a 3D-printed PCL scaffold modified with DMA was found to be a promising substitute for tarsal plate tissue engineering.

Total or subtotal replacement of tarsal plate by novel silicone plate for upper eyelid reconstruction in malignant tumors

“…[ 10 ] Chen et al . [ 19 ] in 2020 uses three-dimensional printed poly-caprolactone scaffolds modified with biomimetic extracellular matrices for tarsal plate tissue engineering. However, the main drawbacks were the requirement of a second surgical site, the uneven thickness of harvested cartilage, and the curvature of the auricular cartilage not coinciding with the lid curvature.…”

Sebaceous gland: Milestones of 30‐year modelling research dedicated to the “brain of the skin”

“…Furthermore, patient‐specific 3D culture models and SKP may open new perspectives in understanding the genetic basis of skin diseases and in a therapeutic context in the field of personalized medicine. [ 50,64,80 ] In the future, such 3D systems could be combined with new scaffold technologies [ 98 ] and microfluidic platforms [ 99 ] allowing precise control and measurement of different culture parameters.…”

“…[ 96 ] More recently, the long‐term expansion of epidermal stem cells in spheroids and organoids [ 50,64,65 ] promises a more efficient culture method for human epidermal stem cells. Meanwhile, current culture methods for SKP are not equally efficient, [ 97 ] and new technologies, such as 3D‐printed scaffolds modified with biomimetic extracellular matrices, [ 98 ] to improve the proliferation and long‐term epigenetic stability of the cells, and microfluidic platforms/organ‐on‐a‐chip technology [ 99 ] are crucial.…”

Aplicações da manufatura aditiva em oftalmologia

“…É composto de densos tecidos conjuntivos, fibras elásticas ricas e um grande número de glândulas meibomianas. Estudo desenvolvido por Chen et al (39) teve como objetivo avaliar o desenvolvimento de células similares aos meibócitos semeadas em uma estrutura de PCL para desenvolvimento por meio da engenharia de tecidos de uma placa tarsal. Citocompatibilidade, adesão celular, proliferação e adipogênese foram verificados in vitro usando sebócitos humanos e in vivo em camundongos com resultados promissores.…”