3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties

Bashiri, Zahra; Fomeshi, Motahareh Rajabi; Hamidabadi, Hatef Ghasemi; Jafari, Davod; Alizadeh, Sanaz; Bojnordi, Maryam Nazm; Orive, Gorka; Dolatshahi-Pirouz, Alireza; Zahiri, Maria; Reis, Rui L.; Kundu, Subhas C.; Gholipourmalekabadi, Mazaher

doi:10.1016/j.mtbio.2023.100666

Cited by 18 publications

(13 citation statements)

References 96 publications

(98 reference statements)

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“…The DPS was fabricated by a protocol described in our recently published work . The porous structure of the DPS, degradation and swelling ratios, mechanical behaviors, and its potential for osteogenic differentiation of MSCs were evaluated in that study .…”

Section: Resultsmentioning

confidence: 99%

Decellularized Placental Sponge: A Platform for Coculture of Mesenchymal Stem Cells/Macrophages to Assess an M2 Phenotype and Osteogenic Differentiation In Vitro and In Vivo

Khosrowpour,

Hashemi,

Mohammadi-Yeganeh

et al. 2024

ACS Omega

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Effective communication between immune and bone-forming cells is crucial for the successful healing of bone defects. This study aimed to assess the potential of a decellularized placental sponge (DPS) as a coculture system for inducing M1/M2 polarization in macrophages and promoting osteogenic differentiation in adipose-derived mesenchymal stem cells (AD-MSCs), both in vitro and in vivo. We prepared the DPS and conducted a comprehensive characterization of its biomechanical properties, antibacterial activity, and biocompatibility. In vitro, we examined the influence of the DPS on the polarization of macrophages cocultured with AD-MSCs through nitric oxide assays, cytokine assays, phagocytosis tests, and real-time polymerase chain reaction (PCR). For in vivo assessment, we utilized micro-CT imaging, histological evaluations, and real-time PCR to determine the impact of the DPS seeded with Wharton's jelly mesenchymal stem cells (WJ-MSCs) on bone regeneration in a calvarial bone defect model. The coculture of AD-MSCs and macrophages on the DPS led to increased production of IL-10, upregulation of CD206, Arg1, and YM1 gene expression, and enhanced phagocytic capacity for apoptotic thymocytes. Concurrently, it reduced the secretion of TNF-α and nitric oxide (NO), downregulated the expression of CD86, NOS2, and IRF5 genes, and decreased macrophage phagocytosis of yeast. These results indicated polarization of macrophages toward an M2-like phenotype. In vivo, the presence of the DPS resulted in enhanced bone formation at the defect site. Immunostaining demonstrated that both the DPS and DPS + WJ-MSC constructs induced macrophage polarization toward an M2 phenotype, as compared to the control defect. In conclusion, this immunomodulatory effect, coupled with its biocompatibility and biomechanical properties resembling natural bone, positions the DPS as an attractive candidate for further exploration in the field of bone tissue engineering and regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

Decellularized Placental Sponge: A Platform for Coculture of Mesenchymal Stem Cells/Macrophages to Assess an M2 Phenotype and Osteogenic Differentiation In Vitro and In Vivo

Khosrowpour,

Hashemi,

Mohammadi-Yeganeh

et al. 2024

ACS Omega

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“…Furthermore, 3D printing provides exact control over material composition and mechanical properties, allowing researchers to tailor scaffold properties to meet specific requirements. 13 Furthermore, the integration of many cell types into a single scaffold is made possible by 3D printing. Because it enables the incorporation of diverse cell populations like keratinocytes, fibroblasts, and endothelial cells, this capability is especially helpful in skin tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

“…This is because 3D printing makes it feasible to design and create intricate geometries that resemble the natural architecture of human skin, allowing for the creation of scaffolds that are specifically tailored to each patient and better support tissue regeneration and cell growth. Furthermore, 3D printing provides exact control over material composition and mechanical properties, allowing researchers to tailor scaffold properties to meet specific requirements . Furthermore, the integration of many cell types into a single scaffold is made possible by 3D printing.…”

Section: Introductionmentioning

confidence: 99%

3D Printing of Alginate/Chitosan-Based Scaffold Empowered by Tyrosol-Loaded Niosome for Wound Healing Applications: In Vitro and In Vivo Performances

Beram,

Ali,

Mesbahian

et al. 2024

ACS Appl. Bio Mater.

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spinning and 3D printing techniques for wound healing applications. The niosomes, measuring 185.40 ± 6.40 nm with a polydispersity index of 0.168 ± 0.012, encapsulated tyrosol with an efficiency of 77.54 ± 1.25%. The scaffold's microsized porous structure (600−900 μm) enhances water absorption, promoting cell adhesion, migration, and proliferation. Mechanical property assessments revealed the scaffold's enhanced resilience, with niosomes increasing the compressive strength, modulus, and strain to failure, indicative of its suitability for wound healing. Controlled tyrosol release was demonstrated in vitro, essential for therapeutic efficacy. The scaffold exhibited significant antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus, with substantial biofilm inhibition and downregulation of bacterial genes (ndvb and icab). A wound healing assay highlighted a notable increase in MMP-2 and MMP-9 mRNA expression and the wound closure area (69.35 ± 2.21%

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“…In terms of skin tissue engineering, grafting material must have sufficient mechanical, biocompatible, and biodegradable, as well as the ability to enable normal tissue repair (Bashiri et al, 2023). In this regard, biological resources based on animal ECM have been used to produce skin substitutes for wound healing, and the decellularization method has achieved a significant level of success (Ghasemi Hamidabadi et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Engineering of a decellularized bovine skin coated with antibiotics‐loaded electrospun fibers with synergistic antibacterial activity for the treatment of infectious wounds

Alizadeh,

Majidi,

Jahani

et al. 2024

Biotech & Bioengineering

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An ideal antibacterial wound dressing with strong antibacterial behavior versus highly drug‐resistant bacteria and great wound‐healing capacity is still being developed. There is a clinical requirement to progress the current clinical cares that fail to fully restore the skin structure due to post‐wound infections. Here, we aim to introduce a novel two‐layer wound dressing using decellularized bovine skin (DBS) tissue and antibacterial nanofibers to design a bioactive scaffold with bio‐mimicking the native extracellular matrix of both dermis and epidermis. For this purpose, polyvinyl alcohol (PVA)/chitosan (CS) solution was loaded with antibiotics (colistin and meropenem) and electrospun on the surface of the DBS scaffold to fabricate a two‐layer antibacterial wound dressing (DBS‐PVA/CS/Abs). In detail, the characterization of the fabricated scaffold was conducted using biomechanical, biological, and antibacterial assays. Based on the results, the fabricated scaffold revealed a homogenous three‐dimensional microstructure with a connected pore network, a high porosity and swelling ratio, and favorable mechanical properties. In addition, according to the cell culture result, our fabricated two‐layer scaffold surface had a good interaction with fibroblast cells and provided an excellent substrate for cell proliferation and attachment. The antibacterial assay revealed a strong antibacterial activity of DBS‐PVA/CS/Abs against both standard strain and multidrug‐resistant clinical isolates of Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli. Our bilayer antibacterial wound dressing is strongly suggested as an admirable wound dressing for the management of infectious skin injuries and now promises to advance with preclinical and clinical research.

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3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties

Cited by 18 publications

References 96 publications

Decellularized Placental Sponge: A Platform for Coculture of Mesenchymal Stem Cells/Macrophages to Assess an M2 Phenotype and Osteogenic Differentiation In Vitro and In Vivo

Decellularized Placental Sponge: A Platform for Coculture of Mesenchymal Stem Cells/Macrophages to Assess an M2 Phenotype and Osteogenic Differentiation In Vitro and In Vivo

3D Printing of Alginate/Chitosan-Based Scaffold Empowered by Tyrosol-Loaded Niosome for Wound Healing Applications: In Vitro and In Vivo Performances

Engineering of a decellularized bovine skin coated with antibiotics‐loaded electrospun fibers with synergistic antibacterial activity for the treatment of infectious wounds

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