3D-Printed Microfluidic Device for the Detection of Pathogenic Bacteria Using Size-based Separation in Helical Channel with Trapezoid Cross-Section

Lee, Won–Jae; Kwon, Donghoon; Choi, Woong; Jung, Gyoo Yeol; Jeon, Sangmin

doi:10.1038/srep07717

Cited by 253 publications

(208 citation statements)

References 36 publications

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“…11 Each structure had a surface area of 6250 mm 2 . A fused deposition modelling (FDM) process was used with a Replicator 2 (MakerBot, USA) printer in poly(lactic) acid (PLA) and uPrint SE (Stratasys, USA) printer in acrylonitrile butadiene styrene (ABS).…”

Section: Additive Manufacturingmentioning

confidence: 99%

Assessment of the biocompatibility of three-dimensional-printed polymers using multispecies toxicity tests

et al. 2015

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Additive manufacturing was adopted in multiple fields of life sciences. It is also becoming a popular tool for rapid prototyping of microfluidic and biomedical devices. Limited studies have been performed to investigate the biological implications of using 3D printed polymers. Here we assessed the biocompatibility of seven commercially available polymers, using a battery of standardized bioassays for chemical risk assessment. Our data show that leachates from photopolymers substrata appear to be very toxic to vertebrates and several invertebrate indicator organisms. These results demonstrate significant consequences for the use of selected photopolymers in the fabrication of bio-devices. V C 2015 AIP Publishing LLC.

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Section: Additive Manufacturingmentioning

confidence: 99%

Assessment of the biocompatibility of three-dimensional-printed polymers using multispecies toxicity tests

et al. 2015

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“…Various groups have successfully demonstrated the fabrication of 3D printed microfluidics devices using different technology platforms for bioanalytical studies [9], fluid handling (e.g. integrated valves and pumps) [10,11], as well as cell processing and separation [12,13]. However, the development of 3D printed microfluidic devices for organs-on-chip applications remains relatively under-explored.…”

Section: Introductionmentioning

confidence: 99%

A 3D printed microfluidic perfusion device for multicellular spheroid cultures

et al. 2017

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The advent of 3D printing technologies promises to make microfluidic organ-on-chip technologies more accessible for the biological research community. To date, hydrogel-encapsulated cells have been successfully incorporated into 3D printed microfluidic devices. However, there is currently no 3D printed microfluidic device that can support multicellular spheroid culture, which facilitates extensive cell–cell contacts important for recapitulating many multicellular functional biological structures. Here, we report a first instance of fabricating a 3D printed microfluidic cell culture device capable of directly immobilizing and maintaining the viability and functionality of 3D multicellular spheroids. We evaluated the feasibility of two common 3D printing technologies i.e. stereolithography (SLA) and PolyJet printing, and found that SLA could prototype a device comprising of cell immobilizing micro-structures that were housed within a microfluidic network with higher fidelity. We have also implemented a pump-free perfusion system, relying on gravity-driven flow to perform medium perfusion in order to reduce the complexity and footprint of the device setup, thereby improving its adaptability into a standard biological laboratory. Finally, we demonstrated the biological performance of the 3D printed device by performing pump-free perfusion cultures of patient-derived parental and metastatic oral squamous cell carcinoma tumor and liver cell (HepG2) spheroids with good cell viability and functionality. This paper presents a proof-of-concept in simplifying and integrating the prototyping and operation of a microfluidic spheroid culture device, which will facilitate its applications in various drug efficacy, metabolism and toxicity studies.

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“…Nanometric details can even help to interact with micro-organisms, not just at a cellular, but also at a molecular level. Potential applications include the development of enhanced organson-chips, the manufacture of biological traps and the development of selective filters capable of capturing bacteria, viruses and pathogens for subsequent studies [29][30][31]. The optimal biological properties of ceramic materials may result in improved performance, as our cell culture results, detailed further on, help to put forward.…”

Section: Resultsmentioning

confidence: 92%

Monolithic 3D labs- and organs-on-chips obtained by lithography-based ceramic manufacture

Lantada

Romero

Schwentenwein³

et al. 2017

Int J Adv Manuf Technol

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In this study, we present a novel approach for the design and development of three-dimensional monolithic ceramic microsystems with complex geometries and with potential applications in the biomedical field, mainly linked to labson-chips and organs-on-chips. The microsystem object of study stands out for its having a complex three-dimensional geometry, for being obtained as a single integrated element, hence reducing components, preventing leakage and avoiding post-processes, and for having a cantilever porous ceramic membrane aimed at separating cell culture chambers at different levels, which imitates the typical configuration of transwell assays. The design has been performed taking account of the special features of the manufacturing technology and includes ad hoc incorporated supporting elements, which do not affect overall performance, for avoiding collapse of the cantilever ceramic membrane during debinding and sintering. The manufacture of the complex three-dimensional microsystem has been accomplished by means of lithography-based ceramic manufacture, the additive manufacturing technology which currently provides the most appealing compromises between overall part size and precision when working with ceramic materials. The microsystem obtained provides one of the most remarkable examples of monolithic bio-microsystems and, to our knowledge, a step forward in the field of ceramic microsystems with complex geometries for lab-on-chip and organ-on-chip applications. Cell culture results help to highlight the potential of the proposed approach and the adequacy of using ceramic materials for biological applications and for interacting at a cellular level.

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3D-Printed Microfluidic Device for the Detection of Pathogenic Bacteria Using Size-based Separation in Helical Channel with Trapezoid Cross-Section

Cited by 253 publications

References 36 publications

Assessment of the biocompatibility of three-dimensional-printed polymers using multispecies toxicity tests

Assessment of the biocompatibility of three-dimensional-printed polymers using multispecies toxicity tests

A 3D printed microfluidic perfusion device for multicellular spheroid cultures

Monolithic 3D labs- and organs-on-chips obtained by lithography-based ceramic manufacture

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