3D Printed Hydrogel Multiassay Platforms for Robust Generation of Engineered Contractile Tissues

Abstract

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A structure-supporting, self-healing, and high permeating hydrogel bioink for establishment of diverse homogeneous tissue-like constructs

“…8 B), it was not easy for the sparse cell clusters to communicate with each other, making GEL-ALG inferior to the TSHSP bioink in terms of nerve tissue repair. In addition to cell distribution, appropriate mechanical external force and extracellular matrix are also beneficial to myogenesis [ 50 , 76 , 77 ]. In this work, we mainly focused on the biological behavior of cells in the new bioink.…”

“…In this work, we mainly focused on the biological behavior of cells in the new bioink. Therefore, we did not apply decellularized extracellular matrix or external force stimuli (such as stretching) as in other reports, resulting no myotubes occurrence in our models [ 50 , 76 , 77 ]. For cartilage engineering, in addition to the above factors, the autocrine effect of cells, which depends on the direct interaction of cell-cell and cell-extracellular matrix, also promotes cartilage regeneration by increasing the size of cell clusters [ 78 ].…”

Bioengineeredin vitroskeletal muscles as new tools for muscular dystrophies preclinical studies

“…In order to mimic the extracellular environment and the native cellular morphology, the main bioengineering strategy is focused on the 3D encapsulation of muscular cell precursors in biocompatible materials. In the last years, 3D bioprinting, [43][44][45][46][47][48][49][50][51][52][53][54] hydrogel molding, [55][56][57][58][59] and microporous scaffolds [60][61][62] have been implemented to fabricate skeletal muscle tissues.…”

“…To mass-produce structures with posts or cantilevers, Christensen et al described a stereolithographic method to 3D print poly(ethylene glycol) diacrylate (PEGDA) hydrogels with high precision and high accuracy (Figure 4(b)). 59 These PEGDA platforms with anchored cantilevers were used to cast fibrin hydrogel muscle bundles around these pillars. Extracellular matrix derived materials obtained by decellularization (dECM) have emerged as novel natural hydrogels to engineer muscle tissue.…”

Coupling 3D Printing and Novel Replica Molding for In House Fabrication of Skeletal Muscle Tissue Engineering Devices

“…[ 20 ] In this case, high throughput was obtained at the expense of versatility, as each variation in size and shape of the pillars would require a new micromilled aluminum mold. Hydrogel 3D printing [ 21 ] involves 3D printers that are expensive, difficult to operate, and not accessible for standard biomedical laboratories. Recently, a set of devices containing small hook‐shaped pillars has been created using a 3D printer, however, this method involved an expensive machine and was only partially successful due to the replica molding strategy based on hard, plastic molds.…”