3D-Printed Chitosan-Based Hydrogels Loaded with Levofloxacin for Tissue Engineering Applications

Koumentakou, Ioanna; Noordam, Michiel Jan; Michopoulou, Anna; Terzopoulou, Zoi; Bikiaris, Dimitrios N.

doi:10.1021/acs.biomac.3c00362

Cited by 9 publications

(5 citation statements)

References 80 publications

(183 reference statements)

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“…The main challenge in the preliminary study was selecting the appropriate combination of materials in which the extract would be encapsulated and present high 3D printability. In the presented work, the influence of different concentrations of chitosan on the properties of the prepared hydrogel and its printing possibilities was assessed, while the concentration of the remaining ingredients remained at the same level [ 24 ]. Previous findings by Zhang et al show a significant dependence of the CS/Gel ratio, where the best osteoblastic responses, including cell adherence progress, spreading area, proliferation, and focal adhesion rate-related gene expression, were demonstrated when CS is in a larger amount than Gel [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

“…Two printing parameters were investigated to ascertain the printability of each hydrogel. To compute the uniformity factor and porosity factor, three distinct areas and fibers were examined using a microscope (ZEISS SteREO Discovery.V20 Motorized Stereo Microscope, Jena, Germany) and ImageJ (https://imagej.net/nih-image/, accessed on 27 March 2023) [24].…”

Section: Preliminary Studies Of 3d-printed Scaffolds Preparationmentioning

confidence: 99%

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3D-Printed Chitosan-Based Scaffolds with Scutellariae baicalensis Extract for Dental Applications

Paczkowska-Walendowska,

Koumentakou,

Lazaridou

et al. 2024

Pharmaceutics

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The plant material Scutellariae baicalensis radix, which is rich in flavones (baicalin), possesses antibacterial, antifungal, antioxidant, and anti-inflammatory properties. This work aimed to develop a 3D-printed chitosan-based hydrogel rich in Scutellariae baicalensis extract as an innovative approach for the personalized treatment of periodontal diseases. Chitosan-based hydrogels were prepared, and the printability of the prepared hydrogels was determined. The hydrogel with 2.5% w/v of high molecular-weight chitosan (CS), 2% w/v gelatin (Gel), and 10% w/w of extract (Ex) presented the best printability, producing smooth and uniform scaffolds. It was proved that the CS/Gel/Ex hydrogel was stabilized by hydrogen bonds and remained in amorphous dispersion in the 3D-printed structures (confirmed by ATR-FTIR and XRPD). Due to the amorphization of the active substance, a significant increase in the release of baicalin in vitro was observed. It was demonstrated that there was an initial burst release and a continuous release profile (n = 3). Higuchi kinetic was the most likely baicalin release kinetic. The second fit, the Korsmeyer–Peppas kinetics model, showed coupled diffusion of the active ingredient in the hydrated matrix and polymer relaxation regulated release, with n values ranging from 0.45 to 0.89. The anti-inflammatory properties of 3D-printed scaffolds were assessed as the ability to inhibit the activity of the hyaluronidase enzyme. Activity was assessed as IC50 = 63.57 ± 4.98 mg hydrogel/mL (n = 6). Cytotoxicity tests demonstrated the biocompatibility of the material. After 24 h of exposure to the 2.5CS/2Gel/10Ex scaffold, fibroblasts migrated toward the scratch, closed the “wound” by 97.1%, and significantly accelerated the wound healing process. The results render the 3D-printed CS/Gel/extract scaffolds as potential candidates for treating periodontal diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Preliminary Studies Of 3d-printed Scaffolds Preparationmentioning

confidence: 99%

3D-Printed Chitosan-Based Scaffolds with Scutellariae baicalensis Extract for Dental Applications

Paczkowska-Walendowska,

Koumentakou,

Lazaridou

et al. 2024

Pharmaceutics

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“…The hydrogels presented good printability and were printed with the use of inkjet-based technology, enabling the production of smooth and uniform scaffolds. Moreover, research has demonstrated the outstanding incorporation of Levofloxacin, which can be continuously released for 48 h [158].…”

Section: Chitosanmentioning

confidence: 99%

An Approach to 3D Printing Techniques, Polymer Materials, and Their Applications in the Production of Drug Delivery Systems

Cardoso,

Araújo

2024

Compounds

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Three-dimensional printing (3DP) technologies are characterized as a set of innovative manufacturing techniques that allow for the creation of complex and/or personalized three-dimensional physical objects on the work surface of a 3D printing machine (based on the computer-aided design (CAD) project designs of these parts). Three-dimensional printing techniques are widely used in various areas of knowledge, such as education, engineering, and biomedicine. Polymeric materials are widely used for these applications, mainly due to their desirable workability during part manufacturing, compatibility with other chemical materials, the wide range of polymers with different physical and chemical characteristics, and the possibility for recycling. The development of polymeric drug delivery systems (DDSs) by 3D printing is currently an active field of research, both in academia and industry, given the potential of this technique for medical purposes. In this context, this work reviews potential polymers for the production of drug delivery systems via 3D printing techniques. The demonstrations of the main 3DP techniques used for drug delivery applications include their working principles and advantages and how the technologies develop the final product. In addition, potential synthetic and natural polymers that are currently used in 3DP drug delivery devices are presented and discussed based on recent scientific studies.

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“…The nitrogen group in its polymer backbone has been reported to act as an excellent electrochemical substrate for energy devices. The N atom acts as dopant that could link to the improvement of the carbon substrate conductivity, contributing to charge transfer within energy devices [ 19 , 147 , 148 ]. Other than that, S atom contained in keratin could also serve the same purpose [ 79 ].…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%

Current significance and future perspective of 3D-printed bio-based polymers for applications in energy conversion and storage system

Putri,

Intasanta,

Hoven

2024

Heliyon

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3D-Printed Chitosan-Based Hydrogels Loaded with Levofloxacin for Tissue Engineering Applications

Cited by 9 publications

References 80 publications

3D-Printed Chitosan-Based Scaffolds with Scutellariae baicalensis Extract for Dental Applications

3D-Printed Chitosan-Based Scaffolds with Scutellariae baicalensis Extract for Dental Applications

An Approach to 3D Printing Techniques, Polymer Materials, and Their Applications in the Production of Drug Delivery Systems

Current significance and future perspective of 3D-printed bio-based polymers for applications in energy conversion and storage system

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