3D Printed Bioconstructs: Regenerative Modulation for Genetic Expression

Shende, Pravin; Trivedi, Riddhi

doi:10.1007/s12015-021-10120-2

Cited by 28 publications

(25 citation statements)

References 64 publications

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“…3D bio-printing of PCL/ HAP/PLGA biomaterials added with gene (DNA, mRNA, miRNA, siRNA, Growth factor: BMP2, etc.) can be used for gene therapy, expression and modulation (Shende and Trivedi, 2021). An adaptive 3D bio-printing approach can be used for depositing material directly over the surface of a free-moving human hand.…”

Section: Biomaterials For Soft and Hard Tissuesmentioning

confidence: 99%

Additive manufacturing techniques for the fabrication of tissue engineering scaffolds: a review

Kumar

Sharma

2021

RPJ

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Purpose Additive manufacturing (AM) or solid freeform fabrication (SFF) technique is extensively used to produce intrinsic 3D structures with high accuracy. Its significant contributions in the field of tissue engineering (TE) have significantly increased in the recent years. TE is used to regenerate or repair impaired tissues which are caused by trauma, disease and injury in human body. There are a number of novel materials such as polymers, ceramics and composites, which possess immense potential for production of scaffolds. However, the major challenge is in developing those bioactive and patient-specific scaffolds, which have a required controlled design like pore architecture with good interconnectivity, optimized porosity and microstructure. Such design not only supports cell proliferation but also promotes good adhesion and differentiation. However, the traditional techniques fail to fulfill all the required specific properties in tissue scaffold. The purpose of this study is to report the review on AM techniques for the fabrication of TE scaffolds. Design/methodology/approach The present review paper provides a detailed analysis of the widely used AM techniques to construct tissue scaffolds using stereolithography (SLA), selective laser sintering (SLS), fused deposition modeling (FDM), binder jetting (BJ) and advanced or hybrid additive manufacturing methods. Findings Subsequently, this study also focuses on understanding the concepts of TE scaffolds and their characteristics, working principle of scaffolds fabrication process. Besides this, mechanical properties, characteristics of microstructure, in vitro and in vivo analysis of the fabricated scaffolds have also been discussed in detail. Originality/value The review paper highlights the way forward in the area of additive manufacturing applications in TE field by following a systematic review methodology.

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Section: Biomaterials For Soft and Hard Tissuesmentioning

confidence: 99%

Additive manufacturing techniques for the fabrication of tissue engineering scaffolds: a review

Kumar

Sharma

2021

RPJ

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“…A bioprinted model mimics vascularization and cellular and microfluidic interactions to improve efficiency in correlating in vivo and in vitro results of drug screening, cancer genesis, metastasis, diagnosis and drug response (52). The cell aggregation in bioprinting can be scaffold-based or scaffold-free wherein scaffold-based bioprinting uses various natural and synthetic polymers that mimic the extracellular environment facilitating cell proliferation and scaffold-free cell aggregation uses a large number of cells that create an extracellular environment to proliferate (53,54). Both types of bioprinted products consisting of tumour cells were studied for multiple applications in cancer research.…”

Section: Bioprinting Tumour Modelsmentioning

confidence: 99%

3D Printed Personalized Medicine for Cancer: Applications for Betterment of Diagnosis, Prognosis and Treatment

2021

Self Cite

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Cancer treatment is challenging due to the tumour heterogeneity that makes personalized medicine a suitable technique for providing better cancer treatment. Personalized medicine analyses patient-related factors like genetic make-up and lifestyle and designs treatments that offer the benefits of reduced side effects and efficient drug delivery. Personalized medicine aims to provide a holistic way for prevention, diagnosis and treatment. The customization desired in personalized medicine is produced accurately by 3D printing which is an established technique known for its precision. Different 3D printing techniques exhibit their capability in producing cancer-specific medications for breast, liver, thyroid and kidney tumours. Three-dimensional printing displays major influence on cancer modelling and studies using cancer models in treatment and diagnosis. Three-dimensional printed personalized tumour models like physical 3D models, bioprinted models and tumour-onchip models demonstrate better in vitro and in vivo correlation in drug screening, cancer metastasis and prognosis studies. Three-dimensional printing helps in cancer modelling; moreover, it has also changed the facet of cancer treatment. Improved treatment via custommade 3D printed devices, implants and dosage forms ensures the delivery of anticancer agents efficiently. This review covers recent applications of 3D printed personalized medicine in various cancer types and comments on the possible future directions like application of 4D printing and regularization of 3D printed personalized medicine in healthcare.Keywords personalized medicine • Tumour-on-chip • 3D bioprinting • 3D printing • Cancer modelling

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“…3D bioprinting is currently widely applied in the fields of tissue or bone regeneration (20,21), neuroblastoma cell culture systems (22), neural catheters or implant engineering (23), vaccine delivery (24), molecular diagnosis (25), surgical models (26), and other fields. In addition, 3D bioprinting technology exhibits significant potential for delivering genes to defective cells in tissue engineering, regenerative medicine, and treating various diseases, especially bone defects (27). The polymer materials used for 3D bioprinting are divided into two categories, namely non-biodegradable polymers and biodegradable polymers.…”

Section: The Application Of Gene Modification Based On 3d Bioprintingmentioning

confidence: 99%

“…It is worth noting that the relationship between the structure of the molecule and its function is crucial. Developing dynamic 3D bioprinting models rely on their biochemical information, while the molecular information provided by nucleic acids, their interactions, functions, and specific mechanisms may lead to enhanced delivery systems (27). However, current DNA synthesis methods face several challenges, such as errors caused by genome sequencing, excessively long oligonucleotides, low yields, time-consuming processes, and the limitations presented by the laboratory environment and conditions (27).…”

Section: Future Trendsmentioning

confidence: 99%

“…Developing dynamic 3D bioprinting models rely on their biochemical information, while the molecular information provided by nucleic acids, their interactions, functions, and specific mechanisms may lead to enhanced delivery systems (27). However, current DNA synthesis methods face several challenges, such as errors caused by genome sequencing, excessively long oligonucleotides, low yields, time-consuming processes, and the limitations presented by the laboratory environment and conditions (27). Therefore, direct gene-printing has attracted increasing attention since it provides a fast, convenient, and efficient way for producing nucleic acids while simultaneously avoiding the limitations mentioned above during the production process.…”

Section: Future Trendsmentioning

confidence: 99%

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Narrative review of gene modification: applications in three-dimensional (3D) bioprinting

Fu¹,

Shen²,

Chen³

et al. 2021

Ann Transl Med

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Objective: This article focused on the application scenarios of three-dimensional (3D) bioprinting and gene-editing technology in various medical fields, including gene therapy, tissue engineering, tumor microenvironment simulation, tumor model construction, cancer regulation and expression, osteogenesis, and skin and vascular regeneration, and summarizing its development prospects and shortcomings.Background: 3D bioprinting is a process based on additive manufacturing that uses biological materials as the microenvironment living cells. The scaffolds and carriers manufactured by 3D bioprinting technology provide a safe, efficient, and economical platform for genes, cells, and biomolecules. Gene modification refers to replacing, splicing, silencing, editing, controlling or inactivating genes and delivering new genes.The combination of this technology that changes cell function or cell fate or corrects endogenous mutations and 3D bioprinting technology has been widely used in various medical field.Methods: We conducted a literature search for papers published up to March 2021 on the gene modification combined with 3D bioprinting in various medical fields via PubMed, Web of Science, China National Knowledge Infrastructure (CNKI). The following medical subject heading terms were included for a MEDLINE search: "3D printing/gene editing", "3D printing/genetic modification", "3D printing/seed cell", "bioprinting/gene editing", "bioprinting/genetic modification", "bioprinting/seed cell", "scaffold/gene editing", "scaffold/genetic modification", "scaffold/seed cell", "gene/scaffold", "gene/bioprinting", "gene/3D printing". Quantitative and qualitative data was extracted through interpretation of each article. Conclusions:We have reviewed the application scenarios of 3D bioprinting and gene-editing technology in various medical fields, it provides an efficient and accurate delivery system for personalized tumor therapy, enhancing the targeting effect while maintaining the integrity of the fabricated structure. It exhibits significant application potential in developing tumor drugs. In addition, scaffolds obtained via 3D bioprinting provide gene therapy applications for skin and bone healing and repair and inducing stem cell differentiation.It also considers the future development direction in this field, such as the emergence and development of

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3D Printed Bioconstructs: Regenerative Modulation for Genetic Expression

Cited by 28 publications

References 64 publications

Additive manufacturing techniques for the fabrication of tissue engineering scaffolds: a review

Additive manufacturing techniques for the fabrication of tissue engineering scaffolds: a review

3D Printed Personalized Medicine for Cancer: Applications for Betterment of Diagnosis, Prognosis and Treatment

Narrative review of gene modification: applications in three-dimensional (3D) bioprinting

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