3D porous PCL-PEG-PCL / strontium, magnesium and boron multi-doped hydroxyapatite composite scaffolds for bone tissue engineering

Yedekçi, Buşra; Tezcaner, Ayşen; Yılmaz, Bengi; Demir, Teyfik; Evis, Zafer

doi:10.1016/j.jmbbm.2021.104941

Cited by 13 publications

(8 citation statements)

References 75 publications

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“…Fabrication of a porous structure with good mechanical properties is a great idea to increase the interchange between consuming and excretory materials, which is necessary for cell metabolism and regrowth. It was also reported before that the presence of porosity in the PCL matrix can enhance the degradability of PCL (Yedekçi, Tezcaner et al 2021).…”

Section: Introductionsupporting

confidence: 55%

In-vitro evaluation of PCL-based film for guiding segmental bone defect

Khodabakhshi

Soleimanimehr

Haghighi

et al. 2022

Preprint

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Segmental bone tissue engineering is a highly effective approach for the repair of large bone defects. In this paper, a PCL-based guide film was developed for controlling segmental bone tissue engineering. 3D bioprinting was used to fabricate the PCL/NaCl-based cylindrical films. The effects of the film's thickness and NaCl concentration on the mechanical properties, degradability, swelling behavior, porosity, and cytotoxicity of the samples were investigated. Response surface methodology was employed to study the mechanical behavior using the central composite design (CCD) results showed that increasing the NaCl concentration up to 10% wt. significantly improved the degradability, swelling, and hydrophilicity of the films. It was also indicated that the maximum stiffness of the guide films under vertical loading was almost 5 times more than the maximum stiffness in the horizontal loading direction, but the samples showed greater compressive strength and elongation under horizontal compressive loading. All the evidence indicated that the mechanical properties of the films were more dependent on the film thickness so the thicker films with an 800µm thickness had better mechanical properties in both vertical and horizontal loading. Cytotoxicity assay also approved the non-toxic effect of the PCL films on the MC3T3 osteoblast cell line. Based on the results, the PCL-based films were a suitable candidate to act as a guide for segmental bone tissue engineering.

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Section: Introductionsupporting

confidence: 55%

In-vitro evaluation of PCL-based film for guiding segmental bone defect

Khodabakhshi

Soleimanimehr

Haghighi

et al. 2022

Preprint

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“…In addition, the particle size and the surface properties of the inorganic phase influence their incorporation within polymer matrices, thus, changing the degradation and mechanical properties of the composites [ 36 ]. Strontium-substituted calcium nano-HA has been recently reported as a component of composite scaffolds based on gelatin [ 45 ], poly(hydroxyalkanoates) [ 46 ], poly(lactide-co-glycolide) [ 47 ], or PCL-poly(ethylene glycol) [ 48 ]. These composite scaffolds have been used in BTE, indicating an enhancement of osteogenic cell differentiation and reduction of the osteoclastic activity.…”

Section: Introductionmentioning

confidence: 99%

Promotion of In Vitro Osteogenic Activity by Melt Extrusion-Based PLLA/PCL/PHBV Scaffolds Enriched with Nano-Hydroxyapatite and Strontium Substituted Nano-Hydroxyapatite

et al. 2023

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Bone tissue engineering has emerged as a promising strategy to overcome the limitations of current treatments for bone-related disorders, but the trade-off between mechanical properties and bioactivity remains a concern for many polymeric materials. To address this need, novel polymeric blends of poly-L-lactic acid (PLLA), polycaprolactone (PCL) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) have been explored. Blend filaments comprising PLLA/PCL/PHBV at a ratio of 90/5/5 wt% have been prepared using twin-screw extrusion. The PLLA/PCL/PHBV blends were enriched with nano-hydroxyapatite (nano-HA) and strontium-substituted nano-HA (Sr-nano-HA) to produce composite filaments. Three-dimensional scaffolds were printed by fused deposition modelling from PLLA/PCL/PHBV blend and composite filaments and evaluated mechanically and biologically for their capacity to support bone formation in vitro. The composite scaffolds had a mean porosity of 40%, mean pores of 800 µm, and an average compressive modulus of 32 MPa. Polymer blend and enriched scaffolds supported cell attachment and proliferation. The alkaline phosphatase activity and calcium production were significantly higher in composite scaffolds compared to the blends. These findings demonstrate that thermoplastic polyesters (PLLA and PCL) can be combined with polymers produced via a bacterial route (PHBV) to produce polymer blends with excellent biocompatibility, providing additional options for polymer blend optimization. The enrichment of the blend with nano-HA and Sr-nano-HA powders enhanced the osteogenic potential in vitro.

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“…Fabrication of a porous structure with good mechanical properties is a great idea to increase the interchange between consuming and excretory materials, which is necessary for cell metabolism and regrowth 15 . It was also reported before that the presence of porosity in the PCL matrix can enhance the degradability of PCL 16 …”

Section: Introductionmentioning

confidence: 99%

“…15 It was also reported before that the presence of porosity in the PCL matrix can enhance the degradability of PCL. 16 PCL in the form of filament for 3D printing approaches like fused deposition modeling (FDM) has some great properties such as inject ability of fused, suitable solidification rate, and self-setting, whereas, in the case of 3D printing, PCL in the form of ink does not have the good bridging capacity to form a suspended layer. 17 In order to solve this problem, the use of a suitable temporary porogen has been reported many times in previous studies.…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of polycaprolactone‐based structure for guiding segmental bone defect

Khodabakhshi,

Soleimanimehr,

Etemadi Haghighi

et al. 2023

Polymer Engineering & Sci

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Segmental bone tissue engineering is a highly effective approach for the repair of large bone defects. In this article, 3D printing was used to fabricate the polycaprolactone (PCL)/NaCl‐based cylindrical structures. The effects of the structure's thickness (L) and NaCl concentration on the mechanical properties, degradability, swelling behavior, porosity, and cytotoxicity of the samples were investigated. Response surface methodology was employed to study the mechanical behavior using the central composite design (CCD). Results showed that increasing the NaCl concentration up to 10% wt significantly improved the degradability, swelling, and hydrophilicity of the structures. It was also indicated that the maximum stiffness of the guide structures under vertical loading was almost five times larger than the horizontal loading direction, but the samples showed greater compressive strength and elongation under horizontal compressive loading. Results indicated that the mechanical properties of the structures were more dependent on the structure thickness so the thicker structures with an 800 μm thickness had better mechanical properties in both vertical and horizontal loading. Cytotoxicity assay also approved the nontoxic effect of the PCL structures on the MC3T3 osteoblast cell line. Based on the results, the PCL‐based structures were a suitable candidate to act as a guide for segmental bone tissue engineering.Highlights A 3D‐printed conduit was manufactured to guide bone reconstruction Response surface methodology (RSM) using central composite design (CCD) was employed NaCl concentration and the diameter of strands were the main parameters This 3D‐printed guiding approach is useful for segmental bone tissue engineering

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3D porous PCL-PEG-PCL / strontium, magnesium and boron multi-doped hydroxyapatite composite scaffolds for bone tissue engineering

Cited by 13 publications

References 75 publications

In-vitro evaluation of PCL-based film for guiding segmental bone defect

In-vitro evaluation of PCL-based film for guiding segmental bone defect

Promotion of In Vitro Osteogenic Activity by Melt Extrusion-Based PLLA/PCL/PHBV Scaffolds Enriched with Nano-Hydroxyapatite and Strontium Substituted Nano-Hydroxyapatite

In vitro evaluation of polycaprolactone‐based structure for guiding segmental bone defect

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