3D porous chitin sponge with high absorbency, rapid shape recovery, and excellent antibacterial activities for noncompressible wound

Fang, Yan; Xu, Yuehua; Wang, Zhiguo; Zhou, Wenjing; Yan, Liyu; Fan, Xuesen; Liu, Haiqing

doi:10.1016/j.cej.2020.124169

Cited by 126 publications

(66 citation statements)

References 44 publications

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“…Interconnected pores of the hemostatic sponge could endow itself with the ability to concentrate blood clotting factors and rapidly recover initial shape 5,10,29 . Moreover, they were able to provide a comfortable niche to support host cell in ltration, vascularization, and tissue ingrowth 30 .…”

Section: Resultsmentioning

confidence: 99%

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Microchannelled chitosan sponge grafting with hydrophobic alkyl chain for enhanced noncompressible hemorrhage and tissue regeneration

Li-jun¹,

Jiang²,

H³

et al. 2020

Preprint

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Developing an anti-infective shape-memory hemostatic sponge with ability of guiding in situ tissue regeneration for noncompressible hemorrhage in civilian and battlefield settings remains a challenge. Here, hemostatic chitosan sponge with highly interconnective microchannels was engineered by combining 3D printed fiber leaching and freeze-drying methods and then modified with hydrophobic alkyl chains. The microchannelled alkylated chitosan sponge (MACS) exhibited a strong capacity for water/blood absorption and rapid shape recovery. Compared to clinically used gauze, gelatin sponge, CELOX, and CELOX-gauze, the MACS demonstrated higher pro-coagulant and hemostatic capacities in lethally normal/heparinized rat and pig liver perforation models. Also, it exhibited strong anti-infective activity against S. aureus and E. coli. Additionally, it promoted liver parenchymal cell infiltration, vascularization, and tissue integration in a rat liver defect model. Overall, the MACS demonstrated promising clinical translational potential in cost effectively treating lethal noncompressible hemorrhage and in facilitating wound healing.

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Section: Resultsmentioning

confidence: 99%

“…1N). Water/blood absorbability of the MACSs The main hemostatic mechanism of expandable hemostats was mechanical compression on the bleeding site, which mainly resulted from water/blood-triggered shape recovery and volume expansion 1,5,14,27,29 . Thus, strong water/blood absorbability was indispensable for expandable hemostats.…”

Section: Resultsmentioning

confidence: 99%

Microchannelled chitosan sponge grafting with hydrophobic alkyl chain for enhanced noncompressible hemorrhage and tissue regeneration

Li-jun¹,

Jiang²,

H³

et al. 2020

Preprint

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“…The haemolysis ratio was determined following a previously reported method [ 38 ]. About 10 mg of sample was immersed into 4.5 mL of normal saline at 37 °C for 24 h to yield the leaching solution.…”

Section: Methodsmentioning

confidence: 99%

Improved hemostatic effects by Fe3+ modified biomimetic PLLA cotton-like mat via sodium alginate grafted with dopamine

Zhang

et al. 2021

Bioactive Materials

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The development of an excellent, bioabsorbable hemostatic material for deep wound remains a challenge. In this work, a biodegradable cotton-like biomimetic fibrous mat of poly ( l -lactic acid) (PLLA) was made by melt spinning. Subsequently, SD composite was prepared by cross-linking sodium alginate (SA) with dopamine (DA). It was immobilized on the fibre surface, which inspired by mussel byssus. Finally, Fe 3+ was loaded onto the 0.5SD/PLLA composite by chelation with the carboxyl of alginate and phenolic hydroxy of dopamine. The haemostasis experiment found that the hemostatic time 47 s in vitro. However, the bleeding volume was 0.097 g and hemostatic time was 23 s when 20Fe 3+ -0.5SD/PLLA was applied in the haemostasis of the rat liver. As a result of its robust hydrophilicity and bouffant cotton-like structure, it could absorb a large water from blood, which could concentrate the component of blood and reduce the clotting time. Furthermore, the addition of Fe 3+ in the 0.5SD/PLLA had a significant effect on improve hemostatic property. It also displayed excellent antibacterial property for Escherichia coli and Staphylococcus aureus . Notably, it possesses superior hemocompatibility, cytocompatibility and histocompatibility. Hence, 20Fe 3+ -0.5SD/PLLA has high potential application in haemostasis for clinical settings due to its outstanding properties.

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Section: Introductionmentioning

confidence: 99%

“…Obtained material exhibited instant water/blood-triggered expansion and superabsorbent capacity. Moreover, authors proved such chitinous 3D sponges attract and stimulate blood cells/platelets, promoting blood coagulation and display superior hemostatic performance to traditional hemostatic materials [52].The discovery of chitin in the skeleton of Cambrian demosponge Vauxia gracilenta (Walcott, 1920) [53], closely related to verongiids, confirmed a million-years-long success of sponges belonging to the order Verongiida, with respect to naturally optimized architecture of their chitinous, robust, fiber-based, microporous skeletons. The content of chitin in verongiids is species-dependent and ranges between 5% and 70% [4,24,27,29,30].…”

mentioning

confidence: 99%

3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo

et al. 2020

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Structure-based tissue engineering requires large-scale 3D cell/tissue manufacture technologies, to produce biologically active scaffolds. Special attention is currently paid to naturally pre-designed scaffolds found in skeletons of marine sponges, which represent a renewable resource of biomaterials. Here, an innovative approach to the production of mineralized scaffolds of natural origin is proposed. For the first time, a method to obtain calcium carbonate deposition ex vivo, using living mollusks hemolymph and a marine-sponge-derived template, is specifically described. For this purpose, the marine sponge Aplysin aarcheri and the terrestrial snail Cornu aspersum were selected as appropriate 3D chitinous scaffold and as hemolymph donor, respectively. The formation of calcium-based phase on the surface of chitinous matrix after its immersion into hemolymph was confirmed by Alizarin Red staining. A direct role of mollusks hemocytes is proposed in the creation of fine-tuned microenvironment necessary for calcification ex vivo. The X-ray diffraction pattern of the sample showed a high CaCO3 amorphous content. Raman spectroscopy evidenced also a crystalline component, with spectra corresponding to biogenic calcite. This study resulted in the development of a new biomimetic product based on ex vivo synthetized ACC and calcite tightly bound to the surface of 3D sponge chitin structure.

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3D porous chitin sponge with high absorbency, rapid shape recovery, and excellent antibacterial activities for noncompressible wound

Cited by 126 publications

References 44 publications

Microchannelled chitosan sponge grafting with hydrophobic alkyl chain for enhanced noncompressible hemorrhage and tissue regeneration

Microchannelled chitosan sponge grafting with hydrophobic alkyl chain for enhanced noncompressible hemorrhage and tissue regeneration

Improved hemostatic effects by Fe3+ modified biomimetic PLLA cotton-like mat via sodium alginate grafted with dopamine

3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo

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