3D PHOVIS: 3D photoacoustic visualization studio

Cho, Seonghee; Baik, Jinwoo; Managuli, Ravi; Kim, Chulhong

doi:10.1016/j.pacs.2020.100168

Cited by 82 publications

(50 citation statements)

References 43 publications

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“…In the case of LV, the peak signal enhancement was about nine folds at 1 min post-injection and then decreases gradually. Figure 4 f shows the volume-rendered image of the overlaid PA/US images, where the SLN and LV are further highlighted with blue color, which is obtained by subtracting the control image from the image at 15 min post-injection [ 45 ] ( Supplementary Video S2 ). The US image depicts the overall structural information of the rat body while the PA image highlights specific structures, including the vasculature, SLN, and LV.…”

Section: Resultsmentioning

confidence: 99%

In Vivo Dual-Modal Photoacoustic and Ultrasound Imaging of Sentinel Lymph Nodes Using a Solid-State Dye Laser System

Han

Choi

Ahn

et al. 2020

Sensors

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Photoacoustic imaging (PAI) is being actively investigated as a non-invasive and non-radioactive imaging technique for sentinel lymph node (SLN) biopsy. By taking advantage of optical and ultrasound imaging, PAI probes SLNs non-invasively with methylene blue (MB) in both live animals and breast cancer patients. However, these PAI systems have limitations for widespread use in clinics and commercial marketplaces because the lasers used by the PAI systems, e.g., tunable liquid dye laser systems and optical parametric oscillator (OPO) lasers, are bulky in size, not economical, and use risky flammable and toxic liquid dyes. To overcome these limitations, we are proposing a novel dual-modal photoacoustic and ultrasound imaging system based on a solid-state dye laser (SD-PAUSI), which is compact, convenient, and carries far less risk of flammability and toxicity. Using a solid-state dye handpiece that generates 650-nm wavelength, we successfully imaged the MB tube positioned deeply (~3.9 cm) in chicken breast tissue. The SLNs were also photoacoustically detected in the in vivo rats beneath a 2.2-cm-thick layer of chicken breast, which is deeper than the typical depth of SLNs in humans (1.2 ± 0.5 cm). Furthermore, we showed the multispectral capability of the PAI by switching the dye handpiece, in which the MB-dyed SLN was selectively highlighted from the surrounding vasculature. These results demonstrated the great potential of the SD-PAUSI as an easy but effective modality for SLN detection.

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Section: Resultsmentioning

confidence: 99%

In Vivo Dual-Modal Photoacoustic and Ultrasound Imaging of Sentinel Lymph Nodes Using a Solid-State Dye Laser System

Han

Choi

Ahn

et al. 2020

Sensors

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“…9 (b) shows three-dimensional (3D) photoacoustic images of blood vessels and gold nanorods at 545 nm and 655 nm, respectively, obtained using 3D Photoacoustic Visualization Studio (3D PHOVIS) software. The 3D PHOVIS is a freely available special 3D visualization software package developed by Cho et al for visualization and rapid processing of photoacoustic data [ 33 ]. We used 3D PHOVIS's volume renderer function to create 3D images of photoacoustic data acquired with excitation wavelengths of 545 nm and 655 nm.…”

Section: Resultsmentioning

confidence: 99%

“…9 (a) In vivo time-lapse molecular overlapped MAP photoacoustic images of the blood vessels and gold nanorods injected in the mouse's ear for 100 seconds. (Supplementary Movie S1) (b) In vivo 3D photoacoustic images of blood vessels and gold nanorods at 545 nm and 655 nm using 3D Photoacoustic Visualization Studio (3D PHOVIS) [ 33 ]. …”

Section: Resultsmentioning

confidence: 99%

Quickly Alternating Green and Red Laser Source for Real-time Multispectral Photoacoustic Microscopy

et al. 2020

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Multispectral photoacoustic microscopy uses a wavelength-dependent absorption difference as a contrast mechanism to image the target molecule. In this paper, we present a novel multispectral pulsed fiber laser source, which selectively alternates the excitation wavelengths between green and red colors based on the stimulated Raman scattering (SRS) effect for imaging. This laser has a high pulse repetition rate (PRR) of 300 kHz and high pulse energy of more than 200 nJ meeting the real-time requirements of optical-resolution photoacoustic microscopy imaging. By switching the polarization state of the pump light and optical paths of the pump light, the operating wavelengths of the light source can be selectively alternated at the same fast PRR for any two SRS peak wavelengths between 545 and 655 nm. At 545 nm excitation wavelength, molecular photoacoustic signals from both blood vessels and gold nanorods were obtained simultaneously. However, at 655 nm, the photoacoustic signals of gold nanorods were dominant because the absorption of light by the blood vessels decreased drastically in the spectral region over 600 nm. Thus the multispectral photoacoustic system designed using the novel laser source implemented here could simultaneously monitor the time-dependent fast movement of two molecules independently, having different wavelength-dependent absorption properties at a high repetition rate of 0.49 frames per second (fps).

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“…3g). Supplementary Movie 2 was made using a specialized 3D visualization software package, namely 3D PA Visualization Studio 65 .…”

Section: Methodsmentioning

confidence: 99%

High-speed three-dimensional photoacoustic computed tomography for preclinical research and clinical translation

et al. 2021

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Photoacoustic computed tomography (PACT) has generated increasing interest for uses in preclinical research and clinical translation. However, the imaging depth, speed, and quality of existing PACT systems have previously limited the potential applications of this technology. To overcome these issues, we developed a three-dimensional photoacoustic computed tomography (3D-PACT) system that features large imaging depth, scalable field of view with isotropic spatial resolution, high imaging speed, and superior image quality. 3D-PACT allows for multipurpose imaging to reveal detailed angiographic information in biological tissues ranging from the rodent brain to the human breast. In the rat brain, we visualize whole brain vasculatures and hemodynamics. In the human breast, an in vivo imaging depth of 4 cm is achieved by scanning the breast within a single breath hold of 10 s. Here, we introduce the 3D-PACT system to provide a unique tool for preclinical research and an appealing prototype for clinical translation.

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3D PHOVIS: 3D photoacoustic visualization studio

Cited by 82 publications

References 43 publications

In Vivo Dual-Modal Photoacoustic and Ultrasound Imaging of Sentinel Lymph Nodes Using a Solid-State Dye Laser System

In Vivo Dual-Modal Photoacoustic and Ultrasound Imaging of Sentinel Lymph Nodes Using a Solid-State Dye Laser System

Quickly Alternating Green and Red Laser Source for Real-time Multispectral Photoacoustic Microscopy

High-speed three-dimensional photoacoustic computed tomography for preclinical research and clinical translation

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