“…This drawback hampers the rational choice of the region of interest for further photoactivation. Nevertheless, due to its monomeric nature, high contrast and high brightness of the activated form, PA-GFP is one of the most powerful PAFPs and has gained popularity in a variety of applications (29,66,88,91,144,161,165,258,269,272,274,297,320,335,377,426,427,461).…”