3D <i>in vivo</i> imaging with extended‐focus optical coherence microscopy

Chen, Yu; Fingler, Jeff; Fraser, Scott E.

doi:10.1002/jbio.201700008

Cited by 5 publications

(2 citation statements)

References 42 publications

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“…OCT imaging data of the human retina were acquired from a custom SD-OCT system (2048-pixel spectrometer) developed at Varocto Inc, as described in our previous work [29]. OCM imaging data of the microbead phantom and zebrafish embryo were acquired from custom spectral-domain OCM (SD-OCM) systems (4096-pixel spectrometer) described in our previous studies [30,31].…”

Section: Oct Imaging Experiments With the Multi-shaping Techniquementioning

confidence: 99%

“…The multi-shaping technique was applied on experimental data obtained from imaging a microbead phantom by our extended-focus OCM (xf-OCM) system [31]. The phantom, used to characterize the performance of the xf-OCM system, consisted of latex microbeads of 0.3μm diameter (LB-3, Sigma-Aldrich) immersed in a 0.75% phytagel solution, with a concentration of ~10 8 particles/mL.…”

Section: Imaging Of Microbead Phantommentioning

confidence: 99%

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Multi-shaping technique reduces sidelobe magnitude in optical coherence tomography

Chen¹,

Fingler²,

Fraser³

2017

Biomed. Opt. Express

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Shaping methods that are commonly used in Fourier-domain optical coherence tomography (FD-OCT) can suppress sidelobe artifacts in the axial direction, but they typically broaden the mainlobe of the point spread function (PSF) and reduce the axial resolution. To improve OCT image quality without this tradeoff, we have developed a multi-shaping technique that reduces the axial sidelobe magnitude dramatically and achieves better axial resolution than conventional shaping methods. This technique is robust and compatible in various FD-OCT imaging systems. Testing of multi-shaping in three experimental settings shows that it reduced the axial sidelobe contribution by more than 8 dB and improved the contrast to noise by at least 30% and up to three-fold. Multi-shaping enables accurate image analysis and is potentially useful in many OCT applications.

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Section: Oct Imaging Experiments With the Multi-shaping Techniquementioning

confidence: 99%

Section: Imaging Of Microbead Phantommentioning

confidence: 99%

Multi-shaping technique reduces sidelobe magnitude in optical coherence tomography

Chen¹,

Fingler²,

Fraser³

2017

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

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Volumetric optical coherence microscopy with a high space-bandwidth-time product enabled by hybrid adaptive optics

Liu

Mulligan

Adie

2018

Biomed. Opt. Express

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Optical coherence microscopy (OCM) is a promising modality for high resolution imaging, but has limited ability to capture large-scale volumetric information about dynamic biological processes with cellular resolution. To enhance the throughput of OCM, we implemented a hybrid adaptive optics (hyAO) approach that combines computational adaptive optics with an intentionally aberrated imaging beam generated via hardware adaptive optics. Using hyAO, we demonstrate the depth-equalized illumination and collection ability of an astigmatic beam compared to a Gaussian beam for cellular-resolution imaging. With this advantage, we achieved volumetric OCM with a higher space-bandwidth- product compared to Gaussian-beam acquisition that employed focus-scanning across depth. HyAO was also used to perform volumetric time-lapse OCM imaging of cellular dynamics over a 1mm × 1mm × 1mm field-of-view with 2 μm isotropic spatial resolution and 3-minute temporal resolution. As hyAO is compatible with both spectral-domain and swept-source beam-scanning OCM systems, significant further improvements in absolute volumetric throughput are possible by use of ultrahigh-speed swept sources.

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Extended-depth-of-field imaging with an ultra-thin folded lens

Zhang,

Wang,

et al. 2024

J. Opt. Soc. Am. A

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Optical systems with extended depth of field (EDOF) are crucial for observation and measurement applications, where achieving compactness and a substantial depth of field (DOF) presents a considerable challenge with conventional optical elements. In this paper, we propose an innovative solution for the miniaturization of EDOF imaging systems by introducing an ultra-thin annular folded lens (AFL). To validate the practical feasibility of the theory, we design an annular four-folded lens with an effective focal length of 80.91 mm and a total thickness of only 8.50 mm. Simulation results show that the proposed folded lens has a DOF of 380.55 m. We further developed an AFL-based test system exhibiting a resolution of 0.11 mrad across a wide wavelength range of 486–656 nm. Additionally, we present experimental results from a miniature compact prototype, which further highlights the promising potential of folded lenses for long-range EDOF imaging.

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3D in vivo imaging with extended‐focus optical coherence microscopy

Cited by 5 publications

References 42 publications

Multi-shaping technique reduces sidelobe magnitude in optical coherence tomography

Multi-shaping technique reduces sidelobe magnitude in optical coherence tomography

Volumetric optical coherence microscopy with a high space-bandwidth-time product enabled by hybrid adaptive optics

Extended-depth-of-field imaging with an ultra-thin folded lens

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