3D hybrid structures based on biomimetic membranes and Caryophyllus aromaticus - “green” synthesized nano-silver with improved bioperformances

Barbinta-Patrascu, Marcela Elisabeta; Badea, Nicoleta; Bacalum, Mihaela; Ungureanu, Camelia; Şuică-Bunghez, Ioana-Raluca; Iordache, Stefan Marian; Pîrvu, Cristian; Zgura, Irina; Maraloiu, Valentin-Adrian

doi:10.1016/j.msec.2019.03.069

Cited by 27 publications

(25 citation statements)

References 78 publications

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“…Cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, as described previously [ 52 ]. First, the cells were seeded in 96-well plates (20,000 cells/well) and cultured for 24 h in medium.…”

Section: Methodsmentioning

confidence: 99%

Copper(II) Complexes with Mixed Heterocycle Ligands as Promising Antibacterial and Antitumor Species

et al. 2020

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Complexes with mixed ligands [Cu(N-N)2(pmtp)](ClO4)2 ((1) N-N: 2,2′-bipyridine; (2) L: 1,10-phenanthroline and pmpt: 5-phenyl-7-methyl-1,2,4-triazolo[1,5-a]pyrimidine) were synthesized and structurally and biologically characterized. Compound (1) crystallizes into space group Pa and (2) in P-1. Both complexes display an intermediate stereochemistry between the two five-coordinated ones. The biological tests indicated that the two compounds exhibited superoxide scavenging capacity, intercalative DNA properties, and metallonuclease activity. Tests on various cell systems indicated that the two complexes neither interfere with the proliferation of Saccharomyces cerevisiae or BJ healthy skin cells, nor cause hemolysis in the active concentration range. Nevertheless, the compounds showed antibacterial potential, with complex (2) being significantly more active than complex (1) against all tested bacterial strains, both in planktonic and biofilm growth state. Both complexes exhibited a very good activity against B16 melanoma cells, with a higher specificity being displayed by compound (1). Taken together, the results indicate that complexes (1) and (2) have specific biological relevance, with potential for the development of antitumor or antimicrobial drugs.

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Section: Methodsmentioning

confidence: 99%

Copper(II) Complexes with Mixed Heterocycle Ligands as Promising Antibacterial and Antitumor Species

et al. 2020

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“…In previous studies, TEM images showed that the silver nanoparticles are coated with liposomes [4], and could be located both in the intervesicular medium and in the vesicular multilamellar structures [24].…”

Section: Preparation Of Silver-based Biocompositesmentioning

confidence: 90%

“…This work develops a "green" bottom-up strategy to achieve antioxidant delivery systems based on vitamin C, biomimetic membranes (liposomes) and biogenic nano-silver synthesized from Arctium lappa leaves aqueous extract. Liposomes are self-assembling vesicles containing one or more lipid bilayers with a unique structure (similar to the structure of biological membranes) conferring them the ability to load both hydrophilic and lipophilic compounds, and also some other interesting properties such as: protection of active agents [1,2], building block for optoelectronic devices [3], and also enhanced stability and improved biological properties (such as: antioxidant, antimicrobial and antiproliferative activities) for silver-based composites [4][5][6][7]. The "green" synthesis of silver nanoparticles using aqueous vegetal extracts is a rapid eco-friendly technique which implies three stages: (i) reduction of silver ions; (ii) growth of AgNPs, and (iii) stabilization of developed metallic nanoparticles [8].…”

Section: Introductionmentioning

confidence: 99%

“…"Green" synthesized silver nanoparticles are recognized for their interesting biological activities. Thus, recent researches summarizes the antioxidant properties of AgNPs phytosynthesized from aqueous extract of Glycyrrhiza glabra (licorice, sweet wood) [9], cloves [4,10] and Citrus peels [5,11,12]. The incorporation of AgNPs in polyurethane nanovesicles [13], liposomes [7,14], or phytosomes [6] resulted in biocomposites with enhanced biological performances.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Silver-based Biogenic Systems Generated from Arctium lappa Leaves

Barbinta-Patrascu¹,

Besliu²,

Meghea³

2020

Rev. Chim.

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This work aims at designing through a �green� bottom-up strategy, four types of bio-based systems containing artificial cell membranes (with or without vitamin C) and silver nanoparticles (AgNPs) phyto-generated from aqueous extract of Arctium lappa leaves. The �green� approaches for AgNP synthesis, by using the vast bioreducing potential of plants are low-cost and eco-friendly procedures.Exploiting vegetal wastes as precursors for phyto-metallic nanoparticles has received attention from scientific community in the last years due to its significant economic and environmental impacts. UV-Vis absorption spectra demonstrated the biosynthesis of metallic nanoparticles which exhibited a strong SPR peak located at 437 nm wavelengths. The formation of the biocomposites containing the �green� synthesized AgNPs has been demonstrated by UV-Vis absorption spectroscopy and Dynamic Light Scattering measurements. DLS results highlighted the nanoscale size of the prepared �green� AgNPs. The physical stability of the obtained silver-based systems was evaluated through zeta potential values, based on electrophoretic mobility. The systems loaded with vitamin C proved to be more stable and showed enhanced antioxidant activities (between 72.3% to 88% evaluated by chemiluminescence technique). Our results demonstrated that these developed silver-based biogenic systems generated from Arctium lappa leaves could be used as antioxidant agents in various biomedical applications.

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“…The biological evaluation was performed through in vitro tests; simulated body fluid (SBF) soaking for 14 days, at 37 • C, the testing solution being prepared according to Kokubo et al [29], as well as MTT assay (cell viability) [30] on human fibroblast BJ cells, the values being read at 24 h.…”

Section: Biological Characterizationmentioning

confidence: 99%

Development of Vitroceramic Coatings and Analysis of Their Suitability for Biomedical Applications

et al. 2019

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Within the field of tissue engineering, thin films have been studied to improve implant fixation of metallic or ceramic materials in bone, connective tissue, oral mucosa or skin. In this context, to enhance their suitability as implantable devices, titanium-based substrates received a superficial vitroceramic coating by means of laser ablation. Further, this study describes the details of fabrication and corresponding tests in order to demonstrate the bioactivity and biocompatibility of the newly engineered surfaces. Thus, the metallic supports were covered with a complex material composed of SiO 2 , P 2 O 5 , CaO, MgO, ZnO and CaF 2 , in the form of thin layers via a physical deposition techniques, namely pulsed laser deposition. The resulting products were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning and transmission electron microscopy coupled with energy dispersive X-ray spectroscopy, selected area electron diffraction, and electron energy loss spectroscopy. It was found that a higher substrate temperature and a lower working pressure lead to the highest quality film. Finally, the samples biocompatibility was assessed and they were found to be bioactive after simulated body fluid soaking and biocompatible through the MTT cell viability test.Coatings 2019, 9, 671 2 of 13 drawbacks, like fibrous tissue growth, slow osseointegration and reduced close contact with the host bone, were noticed upon wider use of titanium alloy implants [19]. Thus, the quest is to develop a composite material with all the mechanical properties of titanium alloys, encapsulated in a leak-proof ceramic, that can be biologically preactivated prior to implant [20,21].With the advent of laser-based additive manufacturing [22], such composite material is now a reality. In this case, a commercial or custom-made ceramic target is ablated by a pulsed laser, a plume of plasma is generated and directed towards the metallic substrate, upon which it settles, forming a thin layer that physically binds to the support [23,24]. Our research group has already reported the growth of nanostructured akermanite-based thin films by pulsed laser deposition, with good biocompatibility and bioactivity [25]. Since both the target characteristics and processing conditions strongly influence the properties of the final coatings [26], more studies are necessary in order to elucidate which are the driven factors and optimized set of parameters.Thus, the aim of this work is to produce a material that is durable and readily implantable. A type of vitroceramic coatings on titanium substrates were developed, characterized and then immersed in simulated biological fluid to be coated with a hydroxyapatite-like layer and demonstrate its bioactivity [27,28]. Further biological tests indicated that, indeed, this combination of materials resulted in an artifact that responds well to the in vitro cell proliferation. Author Contributions: Conceptualization, S.-I.J. and C.B.; methodology, C.B. and D.M.; validation, S.-I.J.; investigation...

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3D hybrid structures based on biomimetic membranes and Caryophyllus aromaticus - “green” synthesized nano-silver with improved bioperformances

Cited by 27 publications

References 78 publications

Copper(II) Complexes with Mixed Heterocycle Ligands as Promising Antibacterial and Antitumor Species

Copper(II) Complexes with Mixed Heterocycle Ligands as Promising Antibacterial and Antitumor Species

Antioxidant Silver-based Biogenic Systems Generated from Arctium lappa Leaves

Development of Vitroceramic Coatings and Analysis of Their Suitability for Biomedical Applications

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