3D Functional Ultrasound Imaging of Pigeons

Rau, Richard; Kruizinga, Pieter; Mastik, Frits; Belau, Markus; Jong, Nico de; Bosch, Johannes G.; Scheffer, Wolfgang; Maret, Georg

doi:10.1101/302323

Cited by 9 publications

(9 citation statements)

References 38 publications

(50 reference statements)

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“…Since its introduction in 2011 17 , fUS has been used to image neural activity during epileptic seizures 17 , olfactory stimuli 21 , and behavioral tasks in freely moving rodents 22,23 . It has also been applied to many non-rodent species including ferrets 24 , pigeons 25 , non-human primates (NHPs) 26 , and humans [27][28][29] . Unlike functional magnetic resonance imaging (fMRI), fUS can be applied in freely moving subjects using miniature, head-mountable transducers.…”

Section: Mainmentioning

confidence: 99%

Single-trial decoding of movement intentions using functional ultrasound neuroimaging

Norman

Maresca

Christopoulos

et al. 2021

Neuron

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Brain-machine interfaces (BMI) are powerful devices for restoring function to people living with paralysis. Leveraging significant advances in neurorecording technology, computational power, and understanding of the underlying neural signals, BMI have enabled severely paralyzed patients to control external devices, such as computers and robotic limbs. However, high-performance BMI currently require highly invasive recording techniques, and are thus only available to niche populations. Here, we show that a minimally invasive neuroimaging approach based on functional ultrasound (fUS) imaging can be used to detect and decode movement intention signals usable for BMI. We trained non-human primates to perform memory-guided movements while using epidural fUS imaging to record changes in cerebral blood volume from the posterior parietal cortex -a brain area important for spatial perception, multisensory integration, and movement planning. Using hemodynamic signals acquired during movement planning, we classified left-cued vs. right-cued movements, establishing the feasibility of ultrasonic BMI. These results demonstrate the ability of fUS-based neural interfaces to take advantage of the excellent spatiotemporal resolution, sensitivity, and field of view of ultrasound without breaching the dura or physically penetrating brain tissue.

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Section: Mainmentioning

confidence: 99%

Single-trial decoding of movement intentions using functional ultrasound neuroimaging

Norman

Maresca

Christopoulos

et al. 2021

Neuron

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“…Functional ultrasound (fUS) has recently emerged as an efficient alternative to BOLD fMRI for functional brain mapping, with millisecond time and mesoscopic (∼100 × 100 × 200 μm) spatial resolution 9,10 . It has been used to investigate olfactory 5,11,12 , visual 13,14 , and somatosensory 9,15,16 responses in anesthetized and awake rodents, and applied to ferrets 17 , pigeons 18 , monkeys 19 and humans 20,21 . In the mouse olfactory bulb (OB), we have recently shown that global fUS sensory responses are tightly correlated to odor 22 .…”

mentioning

confidence: 99%

Transfer functions linking neural calcium to single voxel functional ultrasound signal

et al. 2020

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Functional ultrasound imaging (fUS) is an emerging technique that detects changes of cerebral blood volume triggered by brain activation. Here, we investigate the extent to which fUS faithfully reports local neuronal activation by combining fUS and two-photon microscopy (2PM) in a co-registered single voxel brain volume. Using a machine-learning approach, we compute and validate transfer functions between dendritic calcium signals of specific neurons and vascular signals measured at both microscopic (2PM) and mesoscopic (fUS) levels. We find that transfer functions are robust across a wide range of stimulation paradigms and animals, and reveal a second vascular component of neurovascular coupling upon very strong stimulation. We propose that transfer functions can be considered as reliable quantitative reporters to follow neurovascular coupling dynamics.

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“…This means that the deep cortical folds of the visual cortex within the calcarine sulcus are inaccessible to these techniques (27,28). New functional ultrasound (fUS) imaging techniques have recently been found to provide high spatiotemporal resolution (100 μm, 1 Hz) even in deep structures (up to 1.5 cm) (29,30), and have been applied to three-dimensional (3D) mapping of the visual system of rodents and pigeons (4,29,31). The technique measures changes in the cerebral blood volume (CBV) within microvessels using ultrafast Doppler (32), combined with spatiotemporal clutter filtering based on singularvalue decomposition (33).…”

mentioning

confidence: 99%

Functional ultrasound imaging of deep visual cortex in awake nonhuman primates

Blaize

Arcizet

Gesnik

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Deep regions of the brain are not easily accessible to investigation at the mesoscale level in awake animals or humans. We have recently developed a functional ultrasound (fUS) technique that enables imaging hemodynamic responses to visual tasks. Using fUS imaging on two awake nonhuman primates performing a passive fixation task, we constructed retinotopic maps at depth in the visual cortex (V1, V2, and V3) in the calcarine and lunate sulci. The maps could be acquired in a single-hour session with relatively few presentations of the stimuli. The spatial resolution of the technology is illustrated by mapping patterns similar to ocular dominance (OD) columns within superficial and deep layers of the primary visual cortex. These acquisitions using fUS suggested that OD selectivity is mostly present in layer IV but with extensions into layers II/III and V. This imaging technology provides a new mesoscale approach to the mapping of brain activity at high spatiotemporal resolution in awake subjects within the whole depth of the cortex.

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3D Functional Ultrasound Imaging of Pigeons

Cited by 9 publications

References 38 publications

Single-trial decoding of movement intentions using functional ultrasound neuroimaging

Single-trial decoding of movement intentions using functional ultrasound neuroimaging

Transfer functions linking neural calcium to single voxel functional ultrasound signal

Functional ultrasound imaging of deep visual cortex in awake nonhuman primates

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