3D culturing of human pluripotent stem cells-derived endothelial cells for vascular regeneration

Abstract

Rationale: Human induced pluripotent stem cell-derived endothelial cells can be candidates for engineering therapeutic vascular grafts. Methods: Here, we studied the role of three-dimensional culture on their characteristics and function both in vitro and in vivo . Results: We found that differentiated hPSC-EC can re-populate decellularized biomatrices; they remain viable, undergo maturation and arterial/venous specification. Human PSC-EC develop antifibrotic, vasoactive and anti-inflammatory properties during recellularization. In vivo , a robust increase in perfusion was detected at the engraftment sites after subcutaneous implantation of an hPSC-EC-laden hydrogel in rats. Histology confirmed survival and formation of capillary-like structures, suggesting the incorporation of hPSC-EC into host microvasculature. In a canine model, hiPSC-EC-seeded onto decellularised vascular segments were functional as aortic grafts. Similarly, we showed the retention and maturation of hiPSC-EC and dynamic remodelling of the vessel wall with good maintenance of vascular patency. Conclusions: A combination of hPSC-EC and biomatrices may be a promising approach to repair ischemic tissues.

Experimental conditions for efficient retention of vascular endothelial cells on channel wall using lipid bubbles and acoustic interference

“…5) Although artificial blood vessels have been constructed from vascular cells by forming two-layer microtubes 6) using light-curing resin, the fabrication of vascular features, such as multiple bifurcations, has posed a challenge. Moreover, in the three-dimensional culturing method, where endothelial cells are contained in hydrogel implants, 7) the products were transplanted into rats and dogs to confirm the formation of capillary structures. However, the strength of a monolayer structure of matured vascular endothelial cells is insufficient.…”

Injectable hypoxia-preconditioned cartilage progenitor cells-laden GelMA microspheres system for enhanced osteoarthritis treatment

“…Therefore, it is essential to maintain the stemness and maximize the efficacy of cultured stem cells before harvesting these cells. Three-dimensional (3D) culture technology can mimic a more actual and beneficial physiological environment through cell to cell and cell to matrix interaction [ [19] , [20] , [21] ]. In addition, previous studies have demonstrated that mesenchymal stem cells (MSCs) cultured in 3D condition possess an enhanced stemness and improved therapeutic effects [ 14 , 22 ].…”