3D culture of human pluripotent stem cells in RGD-alginate hydrogel improves retinal tissue development

Abstract

The burden of retinal disease is ever growing with the increasing age of the world-wide population. Transplantation of retinal tissue derived from human pluripotent stem cells (PSCs) is considered a promising treatment. However, derivation of retinal tissue from PSCs using defined media is a lengthy process and often variable between different cell lines. This study indicated that alginate hydrogels enhanced retinal tissue development from PSCs, whereas hyaluronic acid-based hydrogels did not. This is the first study to show that 3D culture with a biomaterial scaffold can improve retinal tissue derivation from PSCs. These findings indicate potential for the clinical application of alginate hydrogels for the derivation and subsequent transplantation retinal tissue. This work may also have implications for the derivation of other pigmented, neural or epithelial tissue.

In situ crosslinkable hydrogels for engineered cellular microenvironments

“…Этот результат показал, что альгинатные инъ-екционные и самовосстанавливающиеся гидрогели обладают большим потенциалом для восстановле-ния центральной нервной системы. Недавно группа Lako разработала альгинатные гидрогели, конъюги-рованные с клеточным адгезивным пептидом RGD (Arg-Gly-Asp) для усиления клеточной активнос-ти гидрогелей [14]. Пептидные звенья ковалент-но встраивали в альгинатный полимерный каркас с последующей сшивкой полимерного хлоридом кальция (CaCl 2 ).…”

“…This result suggested that alginate-based injectable and self-healing hydrogels have great potential in central nervous system repair applications. Recently, the Lako group developed alginate hydrogels conjugated with cell adhesive peptide (Arg-Gly-Asp, RGD) to enhance cellular activity of the hydrogels [14]. They covalently conjugated the peptide sequences into the alginate polymer backbone and fabricated hydrogels using calcium chloride (CaCl 2 ).…”

Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells

“…However, despite these impressive advances, many gaps remain in our understanding of the molecular mechanisms of PR loss, and retinal degenerations remain essentially untreatable. Improvements in the directed differentiation of pluripotent stem cell (PSC) derived retinal cells have shown that PSCs can differentiate into retinal pigment epithelium (RPE) and retinal neurons, including PR cells 2–15 , and can give rise to 3D ‘mini-retinas’, organized into self-forming multi-layered retinal tissues 6, 14–21 . During embryogenesis, the eyefield first appears as an optic vesicle (OV) that evaginates outward from the diencephalon.…”

Recent progress in biomedical applications of RGD‐based ligand: From precise cancer theranostics to biomaterial engineering: A systematic review

“…Taken together, preferential advantages were reported for RGD-alginate scaffold compared to current HA-based hydrogel for retinal cell delivery (Hunt et al, 2017). Indeed, retinal pigmented epithelium (RPE) formation from stem cells has been developed in the presence of RGD alginate scaffold.…”