3D cell-printing of gradient multi-tissue interfaces for rotator cuff regeneration

Chae, Suhun; Yong, Uijung; Park, Wonbin; Choi, Yoo-mi; Kang, Homan; Jang, Jinah; Choi, Hak; Cho, Dong‐Woo

doi:10.1016/j.bioactmat.2022.05.004

Cited by 22 publications

(26 citation statements)

References 69 publications

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“…First, intraoperative NIR fluorescence imaging can differentiate the tumors from nearby normal tissues by visualizing them separately with two different colors. Since the bone-targeted NIR fluorophores are mostly 800 nm emitting, the combination of 700 nm or over 1,000 nm emitting fluorophores targeting other tissues, including cartilage [ 50 ], tendon [ 27 ], nerve [ 51 ], vasculature [ 25 ], and inflammation [ 28 ], enables real-time multispectral imaging and/or the prognostic evaluation of bone metastasis. Second, the photothermal effect of P800SO3-PEG with the features of noninvasiveness and high selectivity makes it useful as a promising adjunct and/or alternative technique to traditional cancer treatments [ 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

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P800SO3-PEG: a renal clearable bone-targeted fluorophore for theranostic imaging

et al. 2022

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Background Due to the deep tissue penetration and reduced scattering, NIR-II fluorescence imaging is advantageous over conventional visible and NIR-I fluorescence imaging for the detection of bone growth, metabolism, metastasis, and other bone-related diseases. Methods Bone-targeted heptamethine cyanine fluorophores were synthesized by substituting the meso-carbon with a sulfur atom, resulting in a bathochromic shift and increased fluorescence intensity. The physicochemical, optical, and thermal stability of newly synthesized bone-targeted NIR fluorophores was performed in aqueous solvents. Calcium binding, bone-specific targeting, biodistribution, pharmacokinetics, and 2D and 3D NIR imaging were performed in animal models. Results The newly synthesized S-substituted heptamethine fluorophores demonstrated a high affinity for hydroxyapatite and calcium phosphate, which improved bone-specific targeting with signal-background ratios > 3.5. Particularly, P800SO3-PEG showed minimum nonspecific uptake, and most unbound molecules were excreted into the urinary bladder. Histological analyses demonstrated that P800SO3-PEG remained stable in the bone for over two weeks and was incorporated into bone matrices. Interestingly, the flexible thiol ethylene glycol linker on P800SO3-PEG induced a promising photothermal effect upon NIR laser irradiation, demonstrating potential theranostic imaging. Conclusions P800SO3-PEG shows a high affinity for bone tissues, deeper tissue imaging capabilities, minimum nonspecific uptake in the major organs, and photothermal effect upon laser irradiation, making it optimal for bone-targeted theranostic imaging.

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Section: Discussionmentioning

confidence: 99%

“…NIR-I images were obtained using the K-FLARE imaging system as described previously in detail [ 24 – 27 ]. Briefly, a 760 nm laser source (11 mW/cm 2 ) was used for excitation, and a 785 nm long-pass (LP) filter was used to collect the NIR-I signal.…”

Section: Methodsmentioning

confidence: 99%

P800SO3-PEG: a renal clearable bone-targeted fluorophore for theranostic imaging

et al. 2022

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“…For the regeneration of the tendon–bone interface (TBI), which is present in rotator cuff (RC) injuries, a 3D bioprinted scaffold mimicking the multi-tissue characteristics of TBI was developed [ 80 ]. The RC is composed of four tendinous entheses consisting of the following three distinct, yet continuous, tissue layers: the tendon, the fibrocartilage, and the bone [ 6 ].…”

Section: Tendon-derived Decm Bioinksmentioning

confidence: 99%

“…The Achilles’ tendons were decellularized and pepsin-digested to a final concentration of 20 mg/mL, obtaining the tendon-derived extracellular matrix (TdECM) bioink. The other bioinks were composed of bone-derived extracellular matrix (BdECM) and polyurethane/poly-caprolactone (PU/PCL) [ 80 ].…”

Section: Tendon-derived Decm Bioinksmentioning

confidence: 99%

Decellularized Extracellular Matrix-Based Bioinks for Tendon Regeneration in Three-Dimensional Bioprinting

Khalak

García‐Villén

Ruiz‐Alonso

et al. 2022

IJMS

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In the last few years, attempts to improve the regeneration of damaged tendons have been rising due to the growing demand. However, current treatments to restore the original performance of the tissue focus on the usage of grafts; although, actual grafts are deficient because they often cannot provide enough support for tissue regeneration, leading to additional complications. The beneficial effect of combining 3D bioprinting and dECM as a novel bioink biomaterial has recently been described. Tendon dECMs have been obtained by using either chemical, biological, or/and physical treatments. Although decellularization protocols are not yet standardized, recently, different protocols have been published. New therapeutic approaches embrace the use of dECM in bioinks for 3D bioprinting, as it has shown promising results in mimicking the composition and the structure of the tissue. However, major obstacles include the poor structural integrity and slow gelation properties of dECM bioinks. Moreover, printing parameters such as speed and temperature have to be optimized for each dECM bioink. Here, we show that dECM bioink for 3D bioprinting provides a promising approach for tendon regeneration for future clinical applications.

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“…NIR uorescence imaging systems NIR-I images were obtained using the K-FLARE imaging system as described previously in detail [22][23][24][25].…”

Section: Calcium Salts Binding Assaymentioning

confidence: 99%

Renal clearable bone-targeted contrast agents for theranostic imaging

Wang

Kang

Dinh

et al. 2022

Preprint

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Background: Due to the deep tissue penetration and reduced scattering, NIR-II fluorescence imaging is advantageous over conventional visible and NIR-I fluorescence imaging for the detection of bone growth, metabolism, metastasis, and other bone-related diseases. Methods: Here, we designed bone-targeted heptamethine cyanine fluorophores by substituting the meso-carbon with a sulfur atom, resulting in a bathochromic shift and increased fluorescence intensity, which together enables NIR-II tail imaging. Results: The newly synthesized S-substituted heptamethine fluorophores demonstrated a high affinity for hydroxyapatite and calcium phosphate, which improved bone-specific targeting with signal-background ratios >3.5. Particularly, P800SO3-PEG showed minimum nonspecific uptake, and most unbound molecules were excreted into the urinary bladder. Histological analyses demonstrated that P800SO3-PEG remained stable in the bone for over two weeks and was incorporated into bone matrices. Interestingly, the flexible thiol ethylene glycol linker on P800SO3-PEG induced a promising photothermal effect, making this bone-targeted agent a potential theranostic imaging agent.Conclusions: P800SO3-PEG shows a high affinity for bone tissues, minimum nonspecific uptake in the major organs, and photothermal effect upon laser irradiation, making it promising as a targeted NIR theranostic agent for bone diseases.

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3D cell-printing of gradient multi-tissue interfaces for rotator cuff regeneration

Cited by 22 publications

References 69 publications

P800SO3-PEG: a renal clearable bone-targeted fluorophore for theranostic imaging

P800SO3-PEG: a renal clearable bone-targeted fluorophore for theranostic imaging

Decellularized Extracellular Matrix-Based Bioinks for Tendon Regeneration in Three-Dimensional Bioprinting

Renal clearable bone-targeted contrast agents for theranostic imaging

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