3D Cell Culture: Techniques For and Beyond Organoid Applications

Sumbalova Koledova, Zuzana

doi:10.1007/978-1-0716-3674-9_1

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…The ability to obtain a high degree of cell organization, cell–cell interactions and ECM components from all these cell types is closely linked to the use of methods and technologies developed to facilitate the formation of 3D cell models capable of mimicking the complexity of tissues and organs. Spheroids and organoids represent the two most important structures where cells are cultured in 3D [ 22 ]. The first is the simplest model of 3D organization, composed of cellular aggregates primarily formed via cell-to cell adhesion derived from cell lines, primary cells or tumor biopsies in mono- or co-cultures.…”

Section: Introductionmentioning

confidence: 99%

“…Microfluidics have also opened up the development of miniaturized cell models, known as organ-on-a-chip models, which combine different organoids with the ultimate goal of better reflecting the physiology of the human organ [ 37 ]. The interested reader may consult the recent reviews in [ 22 , 38 , 39 ] describing the advantages and limits for obtaining 3D models and also the strategies for overcoming them.…”

Section: Introductionmentioning

confidence: 99%

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Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

Bloise,

Giannaccari,

Guagliano

et al. 2024

Cells

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Over the past decade, the development of three-dimensional (3D) models has increased exponentially, facilitating the unravelling of fundamental and essential cellular mechanisms by which cells communicate with each other, assemble into tissues and organs and respond to biochemical and biophysical stimuli under both physiological and pathological conditions. This section presents a concise overview of the most recent updates on the significant contribution of different types of 3D cell cultures including spheroids, organoids and organ-on-chip and bio-printed tissues in advancing our understanding of cellular and molecular mechanisms. The case studies presented include the 3D cultures of breast cancer (BC), endometriosis, the liver microenvironment and infections. In BC, the establishment of 3D culture models has permitted the visualization of the role of cancer-associated fibroblasts in the delivery of exosomes, as well as the significance of the physical properties of the extracellular matrix in promoting cell proliferation and invasion. This approach has also become a valuable tool in gaining insight into general and specific mechanisms of drug resistance. Given the considerable heterogeneity of endometriosis, 3D models offer a more accurate representation of the in vivo microenvironment, thereby facilitating the identification and translation of novel targeted therapeutic strategies. The advantages provided by 3D models of the hepatic environment, in conjunction with the high throughput characterizing various platforms, have enabled the elucidation of complex molecular mechanisms underlying various threatening hepatic diseases. A limited number of 3D models for gut and skin infections have been developed. However, a more profound comprehension of the spatial and temporal interactions between microbes, the host and their environment may facilitate the advancement of in vitro, ex vivo and in vivo disease models. Additionally, it may pave the way for the development of novel therapeutic approaches in diverse research fields. The interested reader will also find concluding remarks on the challenges and prospects of using 3D cell cultures for discovering cellular and molecular mechanisms in the research areas covered in this review.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

Bloise,

Giannaccari,

Guagliano

et al. 2024

Cells

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Rocznica Odkrycia Struktury Dna – Nowe Wyzwania Dla Medycyny

Słomski,

Wielgus,

Danielewski

et al. 2023

frp

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Odkrycie DNA i poznanie jego struktury miało duże znaczenie zarówno na polu naukowym jak i w życiu codziennym. Rozpoznanie DNA jako związku stanowiącego nośnik informacji genetycznej pozwoliło na rozwój genetyki oraz opracowanie metod genetyki molekularnej. Znalazły one zastosowanie m. in. w medycynie, genetyce sądowej i kryminalistyce, diagnostyce molekularnej chorób genetycznych, pozyskiwaniu substancji bioaktywnych z zastosowaniem biotechnologii roślin, analizie archiwalnego DNA (aDNA), innowacyjnej technologii wykorzystania tkanek transgenicznych zwierząt dla celów biomedycznych, transgenicznych zwierzętach jako bioreaktorach oraz zastosowania organizmów modyfikowanych do prewencji chorób. W kwestii zdrowia, analizy DNA pozwoliły na wykrycie podłoża chorób genetycznych, a także przyczyniły się do poznania mechanizmów prowadzących do ich rozwoju. Techniki genetyki molekularnej znacząco ułatwiają i czynią bardziej dokładnym analizy pokrewieństwa i ocenę przynależności śladów biologicznych pozostawionych na miejscu zbrodni. Stale polepszający się warsztat metod analiz DNA umożliwił też postawienie kolejnego kroku milowego w nauce w postaci opracowania technik sekwencjonowania i poznania genomów człowieka oraz wielu innych organizmów. Z kolei opanowanie informacji genomowej dało możliwość produkcji wielu substancji biologicznie aktywnych na skalę przemysłową.

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3D Cell Culture: Techniques For and Beyond Organoid Applications

Cited by 2 publications

References 31 publications

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

Rocznica Odkrycia Struktury Dna – Nowe Wyzwania Dla Medycyny

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