3D Bioprinting Technology and Hydrogels Used in the Process

Lima, Tainara de P. L.; Canelas, Caio Augusto de Almeida; Concha, Víktor Oswaldo Cárdenas; Costa, F.M.; Passos, Marcele Fonseca

doi:10.3390/jfb13040214

Cited by 8 publications

(3 citation statements)

References 186 publications

(186 reference statements)

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“…However, the delicate nature of the hydrogel results in fragile and mechanically unstable constructs. [143] Furthermore, cells in a bio- License. [142] Copyright 2022, The Authors, published by SAGE Publications.…”

Section: Construction Of Biomimetic Organ/tissue Constructmentioning

confidence: 99%

“…However, the delicate nature of the hydrogel results in fragile and mechanically unstable constructs. [ 143 ] Furthermore, cells in a bioprinted construct are usually embedded in crosslinked hydrogel, therefore a diffusion limit, which is ≈100–200 µm for oxygen in hydrogels resulting in insufficient cellular access to nutrients and oxygen, is one of the challenges in the bioprinted construct. [ 19 ]…”

Section: Application Of Nfm Fabrication Techniques For Construction O...mentioning

confidence: 99%

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Recent Progress in Fabrication of Electrospun Nanofiber Membranes for Developing Physiological In Vitro Organ/Tissue Models

Kim,

Youn,

Lee

et al. 2023

Macromolecular Bioscience

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Nanofiber membranes (NFMs), which have an extracellular matrix (ECM)‐mimicking structure and unique physical properties, have garnered great attention as biomimetic materials for developing physiologically relevant in vitro organ/tissue models. Recent progress in NFM fabrication techniques immensely contributed to the development of NFM‐based cell culture platforms for constructing physiological organ/tissue models. However, despite the significance of the NFM fabrication technique, an in‐depth discussion of the fabrication technique and its future aspect was insufficient. This review provides an overview of the current state‐of‐the‐art of NFM fabrication techniques from electrospinning techniques to post‐processing techniques for the fabrication of various types of NFM‐based cell culture platforms. Moreover, the advantages of the NFM‐based culture platforms in the construction of organ/tissue models are discussed especially for tissue barrier models, spheroids/organoids, and biomimetic organ/tissue constructs. Finally, the review concludes with perspectives on challenges and future directions for fabrication and utilization of NFMs.This article is protected by copyright. All rights reserved

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Section: Construction Of Biomimetic Organ/tissue Constructmentioning

confidence: 99%

Section: Application Of Nfm Fabrication Techniques For Construction O...mentioning

confidence: 99%

Recent Progress in Fabrication of Electrospun Nanofiber Membranes for Developing Physiological In Vitro Organ/Tissue Models

Kim,

Youn,

Lee

et al. 2023

Macromolecular Bioscience

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show abstract

“…Three-dimensional bioprinting technology enabled the precise deposition of diverse cell types [20,21], bioinks [22,23], and biomaterials, culminating in the fabrication of intricate tissue [24] and organ constructs with exceptional cellular-level precision. Despite persistent challenges such as sustaining long-term tissue viability and navigating regulatory considerations [25,26], recent advances in 3D bioprinting exhibit immense potential for the prospect of tailored functional tissue replacements and the acceleration of drug development processes.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Optimization of 3D-Printed Silica Scaffolds for Neural Precursor Cell Cultivation

Kastrinaki,

Pechlivani,

Gkekas

et al. 2023

JFB

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The latest developments in tissue engineering scaffolds have sparked a growing interest in the creation of controlled 3D cellular structures that emulate the intricate biophysical and biochemical elements found within versatile in vivo microenvironments. The objective of this study was to 3D-print a monolithic silica scaffold specifically designed for the cultivation of neural precursor cells. Initially, a preliminary investigation was conducted to identify the critical parameters pertaining to calcination. This investigation aimed to produce sturdy and uniform scaffolds with a minimal wall-thickness of 0.5 mm in order to mitigate the formation of cracks. Four cubic specimens, with different wall-thicknesses of 0.5, 1, 2, and 4 mm, were 3D-printed and subjected to two distinct calcination profiles. Thermogravimetric analysis was employed to examine the freshly printed material, revealing critical temperatures associated with increased mass loss. Isothermal steps were subsequently introduced to facilitate controlled phase transitions and reduce crack formation even at the minimum wall thickness of 0.5 mm. The optimized structure stability was obtained for the slow calcination profile (160 min) then the fast calcination profile (60 min) for temperatures up to 900 °C. In situ X-ray diffraction analysis was also employed to assess the crystal phases of the silicate based material throughout various temperature profiles up to 1200 °C, while scanning electron microscopy was utilized to observe micro-scale crack formation. Then, ceramic scaffolds were 3D-printed, adopting a hexagonal and spherical channel structures with channel opening of 2 mm, and subsequently calcined using the optimized slow profile. Finally, the scaffolds were evaluated in terms of biocompatibility, cell proliferation, and differentiation using neural precursor cells (NPCs). These experiments indicated proliferation of NPCs (for 13 days) and differentiation into neurons which remained viable (up to 50 days in culture). In parallel, functionality was verified by expression of pre- (SYN1) and post-synaptic (GRIP1) markers, suggesting that 3D-printed scaffolds are a promising system for biotechnological applications using NPCs.

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Unlocking the future of precision manufacturing: A comprehensive exploration of 3D printing with fiber-reinforced composites in aerospace, automotive, medical, and consumer industries

Tuli,

Khatun,

Rashid

2024

Heliyon

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3D Bioprinting Technology and Hydrogels Used in the Process

Cited by 8 publications

References 186 publications

Recent Progress in Fabrication of Electrospun Nanofiber Membranes for Developing Physiological In Vitro Organ/Tissue Models

Recent Progress in Fabrication of Electrospun Nanofiber Membranes for Developing Physiological In Vitro Organ/Tissue Models

Fabrication and Optimization of 3D-Printed Silica Scaffolds for Neural Precursor Cell Cultivation

Unlocking the future of precision manufacturing: A comprehensive exploration of 3D printing with fiber-reinforced composites in aerospace, automotive, medical, and consumer industries

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