3D bioprinting of hydrogel‐based biomimetic microenvironments

Luo, Yongxiang; Wei, Xu; Huang, Peng

doi:10.1002/jbm.b.34262

Cited by 31 publications

(40 citation statements)

References 108 publications

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“…Notably the rigidity, the surface properties, the chemical/biochemical composition, the permeability to cells and the degradability are critical factors controlling cell fate and tissue homeostasis. For this reason, hydrogels are among the best candidates as inks for printing 3D scaffolds for cell culture [7][8][9][10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

3D printing of a biocompatible low molecular weight supramolecular hydrogel by dimethylsulfoxide water solvent exchange

Chalard

Mauduit

Souleille

et al. 2020

Additive Manufacturing

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A fragile and non-thixotropic biocompatible low molecular weight gel is printed in 3D structures by a solvent exchange process. The 3D printing process is based on the continuous extrusion of a solution of a small amphiphile molecule, N-heptyl-D-galactonamide, in dimethylsulfoxide, that forms a gel in contact with water. The diffusion of water in the dimethylsulfoxide / N-heptyl-D-galactonamide solution triggers the self-assembly of the molecule into supramolecular fibers and the setting of the ink. The conditions for getting a well-defined pattern and the dimensions of the constructs have been determined. The resulting constructs can be easily dissolved, orienting its application as a sacrificial ink or a temporary support. This method opens the way to the injection and the 3D printing of other fragile and non-thixotropic supramolecular hydrogels.

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Section: Introductionmentioning

confidence: 99%

3D printing of a biocompatible low molecular weight supramolecular hydrogel by dimethylsulfoxide water solvent exchange

Chalard

Mauduit

Souleille

et al. 2020

Additive Manufacturing

View full text Add to dashboard Cite

show abstract

“…This strategy has been successful in guiding the fate of microparticle‐adhered cell unitary blocks, or through incorporation in cell‐laden bioinks. The latter is particularly valuable, since the direct incorporation of growth factors in bioinks results in rapid diffusion, often reducing bioactive molecules overall concentration comparing to that achieved with sustained presentation via microparticle‐mediated controlled delivery . Researchers have explored these microparticles features for instance by incorporating gelatin microparticles loaded with either VEGF or BMP‐2 into bioinks for achieving accelerated osteodifferentiation and regional angiogenesis on bioprinted 3D living constructs with well‐defined architecture .…”

Section: Cell–biomaterials Assembliesmentioning

confidence: 99%

Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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Bottom‐up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom‐up assembly of unitary living blocks toward the creation of higher order bioarchitectures based on multicellular‐rich structures or multicomponent cell–biomaterial synergies are described. An up‐to‐date critical overview of long‐term existing and rapidly emerging technologies for integrative bottom‐up tissue engineering is provided, including discussion of their practical challenges and required advances. It is envisioned that a combination of cell–biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues for therapies and disease models, as well as tools for fundamental biological studies.

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“…[6][7][8][9] Taking inspiration from the ECM, which plays an important role not only as a mechanical support but also as a key functional regulator for tissue maturation, considerable efforts have been made to develop hydrogels that recreate the composition or properties of native articular cartilage to guide tissue-specific formation. [10][11][12][13] Currently, tissue-derived dECM (t-dECM) is considered a promising biomaterial since it is a direct way to provide the complex composition of native tissue, which is difficult to reproduce using common biomaterials. Results from studies about cartilage-derived ECM as hydrogel revealed chondro-inductivity and potential for supporting new matrix synthesis, without the need for further functionalization.…”

Section: Introductionmentioning

confidence: 99%

Development of a Biomimetic Hydrogel Based on Predifferentiated Mesenchymal Stem‐Cell‐Derived ECM for Cartilage Tissue Engineering

Antich

Jiménez

Vicente

et al. 2021

Adv Healthcare Materials

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The use of decellularized extracellular matrix (dECM) as a biomaterial has been an important step forward for the development of functional tissue constructs. In addition to tissues and organs, cell cultures are gaining a lot of attention as an alternative source of dECM. In this work, a novel biomimetic hydrogel is developed based on dECM obtained from mesenchymal stem cells (mdECM) for cartilage tissue engineering. To this end, cells are seeded under specific culture conditions to generate an early chondrogenic extracellular matrix (ECM) providing cues and elements necessary for cartilage development. The composition is determined by quantitative, histological, and mass spectrometry techniques. Moreover, the decellularization process is evaluated by measuring the DNA content and compositional analyses, and the hydrogel is formulated at different concentrations (3% and 6% w/v). Results show that mdECM derived hydrogels possess excellent biocompatibility and suitable physicochemical and mechanical properties for their injectability. Furthermore, it is evidenced that this hydrogel is able to induce chondrogenesis of mesenchymal stem cells (MSCs) without supplemental factors and, furthermore, to form hyaline cartilage-like tissue after in vivo implantation. These findings demonstrate for the first time the potential of this hydrogel based on mdECM for applications in cartilage repair and regeneration.

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3D bioprinting of hydrogel‐based biomimetic microenvironments

Cited by 31 publications

References 108 publications

3D printing of a biocompatible low molecular weight supramolecular hydrogel by dimethylsulfoxide water solvent exchange

3D printing of a biocompatible low molecular weight supramolecular hydrogel by dimethylsulfoxide water solvent exchange

Advanced Bottom‐Up Engineering of Living Architectures

Development of a Biomimetic Hydrogel Based on Predifferentiated Mesenchymal Stem‐Cell‐Derived ECM for Cartilage Tissue Engineering

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