3D Bioprinting of Biomimetic Bilayered Scaffold Consisting of Decellularized Extracellular Matrix and Silk Fibroin for Osteochondral Repair

Zhang, Xiao; Liu, Yang; Zuo, Qiang; Wang, Qingyun; Li, Zuxi; Yan, Kai; Yuan, Tao; Zhang, Yi; Shen, Kai; Xie, Rui; Fan, Weimin

doi:10.18063/ijb.v7i4.401

Cited by 26 publications

(11 citation statements)

References 58 publications

(62 reference statements)

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“…Drug-loaded scaffolds were also designed to enhance the regenerative potential of encapsulated cells for OC tissue repair. Among the different biochemical cues, TGFs, BMP and kartogenin (KGN) were the most used for in vivo tissue regeneration [ 52 , 183 , 186 , 187 ]. More in detail, the spatial organization of TGF-β and BMP2 within the cartilage and bone regions, respectively, was exploited to enhance OC regeneration in vivo [ 186 ].…”

Section: In Vivo Advances In the 3d Bioprinting Of Oc Tissuementioning

confidence: 99%

“…Among the different biochemical cues, TGFs, BMP and kartogenin (KGN) were the most used for in vivo tissue regeneration [ 52 , 183 , 186 , 187 ]. More in detail, the spatial organization of TGF-β and BMP2 within the cartilage and bone regions, respectively, was exploited to enhance OC regeneration in vivo [ 186 ]. Reported concentrations of TGF-β were in the range of 0.1–4 µm/mL, while in the case of BMP2, the concentrations were in the range of 4–5 µg/mL [ 186 , 187 ].…”

Section: In Vivo Advances In the 3d Bioprinting Of Oc Tissuementioning

confidence: 99%

“…More in detail, the spatial organization of TGF-β and BMP2 within the cartilage and bone regions, respectively, was exploited to enhance OC regeneration in vivo [ 186 ]. Reported concentrations of TGF-β were in the range of 0.1–4 µm/mL, while in the case of BMP2, the concentrations were in the range of 4–5 µg/mL [ 186 , 187 ]. KGN is a small molecule capable to upregulate chondrogenic gene expression and foster the selective differentiation of BMSCs towards CCs [ 52 , 188 ].…”

Section: In Vivo Advances In the 3d Bioprinting Of Oc Tissuementioning

confidence: 99%

“…As already discussed in previous sections for cartilage and bone, also for the OC tissue, one of the most investigated strategies to improve the mechanical properties of hydrogel scaffolds involves the printing of a cell-laden hydrogel within a thermoplastic framework made of, e.g. PCL [ 25 , 51 , 52 , 186 , 187 , 189 ]. Zhang and co-workers [ 186 ] extruded PCL to print the frame of the bone layer and filled the empty microchannels by printing a bone dECM/SF bioink.…”

Section: In Vivo Advances In the 3d Bioprinting Of Oc Tissuementioning

confidence: 99%

“…PCL [ 25 , 51 , 52 , 186 , 187 , 189 ]. Zhang and co-workers [ 186 ] extruded PCL to print the frame of the bone layer and filled the empty microchannels by printing a bone dECM/SF bioink. The cartilage region was made of a cartilage dECM/SF bioink printed on top of the bone region.…”

Section: In Vivo Advances In the 3d Bioprinting Of Oc Tissuementioning

confidence: 99%

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Integrating bioprinting, cell therapies and drug delivery towards in vivo regeneration of cartilage, bone and osteochondral tissue

Abbadessa,

Ronca,

Salerno

2023

Drug Deliv. and Transl. Res.

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The biological and biomechanical functions of cartilage, bone and osteochondral tissue are naturally orchestrated by a complex crosstalk between zonally dependent cells and extracellular matrix components. In fact, this crosstalk involves biomechanical signals and the release of biochemical cues that direct cell fate and regulate tissue morphogenesis and remodelling in vivo. Three-dimensional bioprinting introduced a paradigm shift in tissue engineering and regenerative medicine, since it allows to mimic native tissue anisotropy introducing compositional and architectural gradients. Moreover, the growing synergy between bioprinting and drug delivery may enable to replicate cell/extracellular matrix reciprocity and dynamics by the careful control of the spatial and temporal patterning of bioactive cues. Although significant advances have been made in this direction, unmet challenges and open research questions persist. These include, among others, the optimization of scaffold zonality and architectural features; the preservation of the bioactivity of loaded active molecules, as well as their spatio-temporal release; the in vitro scaffold maturation prior to implantation; the pros and cons of each animal model and the graft-defect mismatch; and the in vivo non-invasive monitoring of new tissue formation. This work critically reviews these aspects and reveals the state of the art of using three-dimensional bioprinting, and its synergy with drug delivery technologies, to pattern the distribution of cells and/or active molecules in cartilage, bone and osteochondral engineered tissues. Most notably, this work focuses on approaches, technologies and biomaterials that are currently under in vivo investigations, as these give important insights on scaffold performance at the implantation site and its interaction/integration with surrounding tissues. Graphical Abstract

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Section: In Vivo Advances In the 3d Bioprinting Of Oc Tissuementioning

confidence: 99%

Section: In Vivo Advances In the 3d Bioprinting Of Oc Tissuementioning

confidence: 99%

Section: In Vivo Advances In the 3d Bioprinting Of Oc Tissuementioning

confidence: 99%

Section: In Vivo Advances In the 3d Bioprinting Of Oc Tissuementioning

confidence: 99%

Section: In Vivo Advances In the 3d Bioprinting Of Oc Tissuementioning

confidence: 99%

See 3 more Smart Citations

Integrating bioprinting, cell therapies and drug delivery towards in vivo regeneration of cartilage, bone and osteochondral tissue

Abbadessa,

Ronca,

Salerno

2023

Drug Deliv. and Transl. Res.

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A Review of Composition–Structure–Function Properties and Tissue Engineering Strategies of Articular Cartilage: Compare Condyle Process and Knee Joint

Liu

Zhang

et al. 2022

Adv Eng Mater

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Temporomandibular joint (TMJ) and knee joint are two of the most important joints in human body. The TMJ, connecting mandible and skull, regulates mandibular movement. The movable circular upper end of the mandible is called condyle, and the upper concave part above the condyle is called articular fossa. Between condyle and articular fossa is a disc composed of fibrocartilage, which is called articular disc. The articular disc has a buffering effect of absorbing pressure, which makes the condyle easy to move in opening, closing, and chewing movement. [1][2][3] Knee joint is the largest and most complex joint in human body, composed of lower tibia, upper femur, and patella. Placed between the articular surfaces of the medial and lateral condyles of the femur and tibia, the semilunar fibrocartilage structure, called meniscus, can buffer the vibration of the joint and avoid direct bone friction. [4] The condylar cartilage of TMJ and the tibial cartilage of knee joint are highly comparable because they are in similar positions and mechanical environment. The superficial layer of condylar cartilage is fibrocartilage, and its deeper layer is hyaline-like cartilage. The hyaline cartilage rich in proteoglycan is covered with surface fibrocartilage layer rich in collagen. [5] However, the tibial artilage is consistent with most articular cartilage and belongs to hyaline cartilage.In terms of biomechanics, there are differences and similarities in the test methods of condylar cartilage and tibial plateau cartilage. Both use dynamic thermomechanical analysis (DMA) and nanoindentation technology to analyze the modulus of cartilage under dynamic compression, and establish finite element models to simulate the biomechanical state of real cartilage. [6,7] For condylar cartilage, indentation creep test is often used to test its compressive properties, which is a way to extract the data of aggregate modulus, Poisson's ratio, and solid matrix permeability by fitting creep data with curves. [8] Other research methods, such as dynamic small strain shear test using automatic dynamic viscoelastometer, have also been applied to measuring the biomechanical properties of condylar cartilage. Almost all biomechanical experiments of condylar cartilage are in vitro. [9] However, for the tibial cartilage, the stress force applied in the test is mostly the stress under the natural motion state, such as walking and jumping. And most of the experiments are in vivo. After a certain motion stimulus is applied, the specific strain response is measured by magnetic resonance imaging (MRI)

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Remote Magnetic Microengineering and Alignment of Spheroids into 3D Cellular Fibers

Demri

Dumas

Nguyen

et al. 2022

Adv Funct Materials

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Developing in vitro models that recapitulate the in vivo organization of living cells in a 3D microenvironment is one of the current challenges in the field of tissue engineering. In particular for anisotropic tissues where alignment of precursor cells is required for them to create functional structures. Herein, a new method is proposed that allows aligning in the direction of a uniform magnetic field both individual cells (muscle, stromal, and stem cells) or spheroids in a thermoresponsive collagen hydrogel. In an all-in-one approach, spheroids are generated at high throughput by magnetic engineering using microfabricated micromagnets and are used as building blocks to create 3D anisotropic tissue structures of different scales. The magnetic cells and spheroids alignment process is optimized in terms of magnetic cell labeling, concentration, and size. Anisotropic structures are induced to form fibers in the direction of the magnetic alignment, with the respective roles of the magnetic field, the mechanical stretching of hydrogel or co-culture of the aligned cells with non-magnetic stromal cells, being investigated. Over days, spheroids fuse into 3D tubular structures, oriented in the direction of the magnetic alignment. Moreover, in the case of the muscle cells model, multinucleated cells can be observed within the fibers.

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3D Bioprinting of Biomimetic Bilayered Scaffold Consisting of Decellularized Extracellular Matrix and Silk Fibroin for Osteochondral Repair

Cited by 26 publications

References 58 publications

Integrating bioprinting, cell therapies and drug delivery towards in vivo regeneration of cartilage, bone and osteochondral tissue

Integrating bioprinting, cell therapies and drug delivery towards in vivo regeneration of cartilage, bone and osteochondral tissue

A Review of Composition–Structure–Function Properties and Tissue Engineering Strategies of Articular Cartilage: Compare Condyle Process and Knee Joint

Remote Magnetic Microengineering and Alignment of Spheroids into 3D Cellular Fibers

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