3D Bioprinting in Skin Related Research: Recent Achievements and Application Perspectives

Olejnik, Anna; Semba, Julia Anna; Kulpa, Adam; Dańczak‐Pazdrowska, Aleksandra; Rybka, Jakub Dalibor; Gornowicz‐Porowska, Justyna

doi:10.1021/acssynbio.1c00547

Cited by 52 publications

(43 citation statements)

References 134 publications

(274 reference statements)

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“…The demonstration that the bioartificial skin could protect the patient’s inner tissues from the incoming UV light supports the clinical use of the UGRSKIN model. The suitability of this skin model should be compared with other types of skin substitutes generated by 3D printing or other biofabrication methods [ 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range

et al. 2022

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The most recent generation of bioengineered human skin allows for the efficient treatment of patients with severe skin defects. Despite UV sunlight can seriously affect human skin, the optical behavior in the UV range of skin models is still unexplored. In the present study, absorbance and transmittance of the UGRSKIN bioartificial skin substitute generated with human skin cells combined with fibrin-agarose biomaterials were evaluated for: UV-C (200–280 nm), -B (280–315 nm), and -A (315–400 nm) spectral range after 7, 14, 21 and 28 days of ex vivo development. The epidermis of the bioartificial skin substitute was able to mature and differentiate in a time-dependent manner, expressing relevant molecules able to absorb most of the incoming UV radiation. Absorbance spectral behavior of the skin substitutes showed similar patterns to control native skin (VAF > 99.4%), with values 0.85–0.90 times lower than control values at 7 and 14- days and 1.05–1.10 times the control values at 21- and 28-days. UV absorbance increased, and UV transmission decreased with culture time, and comparable results to the control were found at 21 and 28 days. These findings support the use of samples corresponding to 21 or 28 days of development for clinical purposes due to their higher histological similarities with native skin, but also because of their absorbance of UV radiation.

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Section: Discussionmentioning

confidence: 99%

Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range

et al. 2022

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“…Since then tremendous advances have been reported in the field of 3D bioprinting technologies in general and 3D bioprinting of skin equivalents. Several recent reviews give very detailed insight into the role of different printing technologies, as well as into the use of different structural components, i.e., biomaterials or bioinks, and various cell types [ 99 , 123 , 127 , 128 ].…”

Section: Human Skin Equivalentsmentioning

confidence: 99%

“…Pressure-based bioprinting, or extrusion bioprinting, is the most popular technique for skin tissue engineering as it allows a wide range of biomaterials with different viscosities being printed at room temperature. The technique of pressure-based bioprinting applies pneumatic pressure or mechanical pistons for continuous deposition of the biomaterials [ 127 , 128 , 129 ]. Inkjet-based bioprinting uses a drop-on-demand printing mode usually mediated by thermal or piezoelectric effects [ 130 ].…”

Section: Human Skin Equivalentsmentioning

confidence: 99%

Modelling the Complexity of Human Skin In Vitro

et al. 2023

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The skin serves as an important barrier protecting the body from physical, chemical and pathogenic hazards as well as regulating the bi-directional transport of water, ions and nutrients. In order to improve the knowledge on skin structure and function as well as on skin diseases, animal experiments are often employed, but anatomical as well as physiological interspecies differences may result in poor translatability of animal-based data to the clinical situation. In vitro models, such as human reconstructed epidermis or full skin equivalents, are valuable alternatives to animal experiments. Enormous advances have been achieved in establishing skin models of increasing complexity in the past. In this review, human skin structures are described as well as the fast evolving technologies developed to reconstruct the complexity of human skin structures in vitro.

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“…Finally, 3D-bioprinted full-functional skin substitute with nerve, vessel and lymph integration is still a theoretical model in lab. The full-function relies on the coordination of bioprinted skin appendages with recipient nerve, vessel and lymph [ 190 ]. For example, bioprinted sweat glands embedded in skin substitutes could be functionalized by recipient neural control and sufficient blood supply for exocrine sweat [ 191 ].…”

Section: Limitationsmentioning

confidence: 99%

Advances in 3D skin bioprinting for wound healing and disease modeling

Zhang

Zhao

et al. 2022

Regenerative Biomaterials

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Even with many advances in design strategies over the past three decades, an enormous gap remains between existing tissue engineering skin and natural skin. Currently available in vitro skin models still cannot replicate the three-dimensionality and heterogeneity of the dermal microenvironment sufficiently to recapitulate many of the known characteristics of skin disorder or disease in vivo. Three-dimensional (3D) bioprinting enables precise control over multiple compositions, spatial distributions, and architectural complexity, therefore offering hope for filling the gap of structure and function between natural and artificial skin. Our understanding of wound healing process and skin disease would thus be boosted by the development of in vitro models that could more completely capture the heterogeneous features of skin biology. Here we provide an overview of recent advances in 3D skin bioprinting, as well as design concepts of cells and bioinks suitable for the bioprinting process. We focus on the applications of this technology for engineering physiological or pathological skin model, focusing more specifically on the function of skin appendages and vasculature. We conclude with current challenges and the technical perspective for further development of 3D skin bioprinting.

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3D Bioprinting in Skin Related Research: Recent Achievements and Application Perspectives

Cited by 52 publications

References 134 publications

Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range

Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range

Modelling the Complexity of Human Skin In Vitro

Advances in 3D skin bioprinting for wound healing and disease modeling

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