3D bioprinter applied picosecond pulsed electric fields for targeted manipulation of proliferation and lineage specific gene expression in neural stem cells

Petrella, Ross Aaron; Mollica, Peter A.; Zamponi, Martina; Reid, John A.; Xiao, Shu; Bruno, Robert D.; Sachs, Patrick C.

doi:10.1088/1741-2552/aac8ec

Cited by 15 publications

(32 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the effect of pulsed ES on stem cell proliferation is cell type specific. Petrella et al [ 38 ] compared the effects of picosecond pulsed electric field on NSCs and MSCs. Pulsed ES has no influence on MSC proliferation but improves NSC proliferation and astrocyte-specific differentiation by upregulating GFAP after 24 h under 40 kV/cm.…”

Section: Electrical Stimulation Enhances Stem Cell Neural Differentiationmentioning

confidence: 99%

“…An amine functionalized MWCNT and polyethylene glycol dipropionate (PEGDA) polymer composite complex was fabricated into a tunable porous neural scaffold that could promote neural stem cell proliferation and neuronal differentiation via a stereolithography 3D printer [ 77 ]. Petrella et al used a 3D printer anchored with a picosecond pulse electric field electrode to print MSCs and NSCs [ 38 ]. Their data indicated that 40 kV/cm at 1800 pulses can promote astrocyte specific differentiation but not alter differentiation of MSCs.…”

Section: Effect Of Electrical Stimulation Through Conductive Materials On Neural Differentiationmentioning

confidence: 99%

See 1 more Smart Citation

Electrical Stimulation Promotes Stem Cell Neural Differentiation in Tissue Engineering

Cheng

Huang

Yue

et al. 2021

Stem Cells International

View full text Add to dashboard Cite

Nerve injuries and neurodegenerative disorders remain serious challenges, owing to the poor treatment outcomes of in situ neural stem cell regeneration. The most promising treatment for such injuries and disorders is stem cell-based therapies, but there remain obstacles in controlling the differentiation of stem cells into fully functional neuronal cells. Various biochemical and physical approaches have been explored to improve stem cell-based neural tissue engineering, among which electrical stimulation has been validated as a promising one both in vitro and in vivo. Here, we summarize the most basic waveforms of electrical stimulation and the conductive materials used for the fabrication of electroactive substrates or scaffolds in neural tissue engineering. Various intensities and patterns of electrical current result in different biological effects, such as enhancing the proliferation, migration, and differentiation of stem cells into neural cells. Moreover, conductive materials can be used in delivering electrical stimulation to manipulate the migration and differentiation of stem cells and the outgrowth of neurites on two- and three-dimensional scaffolds. Finally, we also discuss the possible mechanisms in enhancing stem cell neural differentiation using electrical stimulation. We believe that stem cell-based therapies using biocompatible conductive scaffolds under electrical stimulation and biochemical induction are promising for neural regeneration.

show abstract

Section: Electrical Stimulation Enhances Stem Cell Neural Differentiationmentioning

confidence: 99%

Section: Effect Of Electrical Stimulation Through Conductive Materials On Neural Differentiationmentioning

confidence: 99%

Electrical Stimulation Promotes Stem Cell Neural Differentiation in Tissue Engineering

Cheng

Huang

Yue

et al. 2021

Stem Cells International

View full text Add to dashboard Cite

show abstract

“…However, limited by the technique of the picosecond pulse generator, psPEFs, especially their potential electro‐biological effects, have rarely been studied. Petrella et al [2018] noted the promising application of a 730 ps, 1016 V/m psPEF in neural stimulation and neural stem cell proliferation. In addition, our simulation work found that picosecond voltage pulse matching an impulse‐radiated antenna could achieve a focus in breast tissue, and when the input was 1 V Gaussian pulse (200 ps in pulse width), the intensity of the focal spots was 62.8 V/m [Li et al, 2018b], which further indicated the promising potential of psPEFs in noninvasive neuromodulation.…”

Section: Introductionmentioning

confidence: 99%

Picosecond Pulse Electrical Field Suppressing Spike Firing in Hippocampal CA1 in Rat In Vivo

Wang

Zhang³

et al. 2020

Bioelectromagnetics

View full text Add to dashboard Cite

Picosecond pulse electrical fields (psPEFs), due to their high temporal-resolution accuracy and localization, were viewed as a potential targeted and noninvasive method for neuromodulation. However, few studies have reported psPEFs regulating neuronal activity in vivo. In this paper, a preliminary study on psPEFs regulating action potentials in hippocampus CA1 of rats in vivo was carried out. By analyzing the neuronal spike firing rate in hippocampus CA1 pre-and post-psPEF stimulation, effects of frequency, duration, and dosimetry of psPEFs were studied. The psPEF used in this study had a pulse width of 500 ps and a field strength of 1 kV/mm, established by 1 kV picosecond voltage pulses. Results showed that the psPEF suppressed spike firing in hippocampal CA1 neurons. The suppression effect was found to be significant except for 10 s, 10 Hz. For shortduration stimulation (10 s), the inhibition rate of spike firing increased with frequency. At longer stimulation durations (1 and 2 min), the inhibition rate increased and decreased alternately as the frequency increased. Despite this, the inhibition rate at high frequencies (5 and 10 kHz) was significantly larger than that at 10 and 100 Hz. A cumulative effect of psPEF on spike firing inhibition was found at low frequencies (10 and 100 Hz), which was saturated when frequency reached 500 Hz or higher. This paper conducts a study on psPEF regulating spike firing in hippocampal CA1 in vivo for the first time and guides subsequent study on psPEF achieving noninvasive neuromodulation. Bioelectromagnetics. 2020;41:617-629.

show abstract

“…Anesthetics are currently drugs with the greatest direct impact on the nerves, and are applied most frequently. As a commonly-used anesthetic, sevoflurane used to be considered as a landmark drug in inhalation anesthesia (7), which is characterized by low dose of muscle relaxant and rapid postoperative recovery, and it is often used in anesthesia for children (8,9). However, there have been different reports on the effect of sevoflurane on nerves so far.…”

Section: Introductionmentioning

confidence: 99%

Toxicity mechanism of sevoflurane in neural stem cells of rats through DNA methylation

Tian

Zhang

et al. 2019

Exp Ther Med

View full text Add to dashboard Cite

The present study investigated the influence of sevoflurane on the cytotoxicity of neural stem cells of rats and deoxyribonucleic acid (DNA) methylation, and analyzed the correlation between degree of methylation and neurotoxicity of sevoflurane. Ten healthy Sprague-Dawley rats aged 6–8 weeks were randomly selected. The neural stem cells in the hippocampus of rats were isolated, followed by multiplication culture and induced differentiation. The nerve-related factors were observed and detected under a microscope. Moreover, the neural stem cells were treated with sevoflurane in different concentrations. Three wells were only added with the normal medium as the control group (C 0 ), 3 wells were added with the low-concentration sevoflurane (0.2 g/ml) prepared by the medium as the low-concentration group (C 1 ), 3 wells were added with the moderate-concentration of sevoflurane (0.5 g/ml) as the moderate-concentration group (C 2 ), and 3 wells were added with the high-concentration sevoflurane (1 g/ml) as the high-concentration group (C 3 ). The apoptosis rate was detected and calculated via Cell Counting Kit-8 (CCK-8) assay, the content of genomic DNA methylation in neural stem cells in each group was detected via high-performance liquid chromatography (HPLC), and the distribution of methylation in the chromosome in each group was compared. During the culture, neurospheres were produced, and the expression levels of four neural markers were increased. With the increase of sevoflurane concentration and the prolongation of time, the apoptosis rate of stem cells was increased. The content of methylation in cells treated with sevoflurane in a higher concentration was higher than that in other groups (P<0.05). According to the Pearsons correlation analysis, the content of methylation in neural stem cells was directly proportional to the concentration of sevoflurane. Methylation mostly occurred in the autosome, and the content of methylation in the high-concentration group was higher than those in the moderate-concentration, low-concentration and control groups (P<0.05). In conclusion, the concentration of sevoflurane can affect the degree of methylation in neural stem cells of rats and produce certain cytotoxicity.

show abstract

3D bioprinter applied picosecond pulsed electric fields for targeted manipulation of proliferation and lineage specific gene expression in neural stem cells

Abstract: Changes in cell metabolism, proliferation, and gene expression after picosecond pulsed electric field exposure are cell type specific.

Cited by 15 publications

References 41 publications

Electrical Stimulation Promotes Stem Cell Neural Differentiation in Tissue Engineering

Electrical Stimulation Promotes Stem Cell Neural Differentiation in Tissue Engineering

Picosecond Pulse Electrical Field Suppressing Spike Firing in Hippocampal CA1 in Rat In Vivo

Toxicity mechanism of sevoflurane in neural stem cells of rats through DNA methylation

Contact Info

Product

Resources

About