2021
DOI: 10.1088/1758-5090/abff11
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3D bioprinted human iPSC-derived somatosensory constructs with functional and highly purified sensory neuron networks

Abstract: Engineering three-dimensional (3D) sensible tissue constructs, along with the complex microarchitecture wiring of the sensory nervous system, has been an ongoing challenge in the tissue engineering field. By combining 3D bioprinting and human pluripotent stem cell (hPSC) technologies, sensible tissue constructs could be engineered in a rapid, precise, and controllable manner to replicate 3D microarchitectures and mechanosensory functionalities of the native sensory tissue (e.g. response to external stimuli). H… Show more

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Cited by 13 publications
(9 citation statements)
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“…To evaluate cellular behaviors in the GCG support gels, gelatin and GelMA were mixed in different ratios, such as 1:3 (25:75 GCG), 2:2 (50:50 GCG), and 0:1 (0:100 GCG), and then cross-linked by UV light to make stable hydrogels in biological conditions. In our previous study, bulk GelMA prepolymer solution as a thermal healing supporting bath was used as an ECM-mimic for fabricating neurospheroid brain-like constructs. Here, we emphasize the ECM-mimic function of the GCG support baths compared with the bulk GelMA hydrogel.…”
Section: Resultsmentioning
confidence: 99%
“…To evaluate cellular behaviors in the GCG support gels, gelatin and GelMA were mixed in different ratios, such as 1:3 (25:75 GCG), 2:2 (50:50 GCG), and 0:1 (0:100 GCG), and then cross-linked by UV light to make stable hydrogels in biological conditions. In our previous study, bulk GelMA prepolymer solution as a thermal healing supporting bath was used as an ECM-mimic for fabricating neurospheroid brain-like constructs. Here, we emphasize the ECM-mimic function of the GCG support baths compared with the bulk GelMA hydrogel.…”
Section: Resultsmentioning
confidence: 99%
“…Results from previous studies indicate that gelatin can be a suitable biomatrix for 3D bioprinting of stem cells [ 33 , 34 , 35 , 36 , 37 , 38 ] and may even in itself promote their neural differentiation [ 39 , 40 , 41 ]. The results suggest that culturing BC in gelatin-based hydrogels, which are enzymatically cross-linked with mTG, do not have a negative impact on viability compared to BC cultures on pre-cross-linked gelatin and reference cultures.…”
Section: Discussionmentioning
confidence: 99%
“…A CNS–peripheral nervous system model, generated with specialized dorsal horn sensory neurons responsive to µ-opioid receptor activators and inhibitors, can be applied to study ascending spinal sensory pathways 206 . Similarly, neuro-mesodermal assembloids and 3D-bioprinted somatosensory constructs respond to capsaicin and menthol, recapitulating the receptive features of several pathways governing pain, temperature and taste 207 , 208 .…”
Section: Region-specific Cns Modelsmentioning
confidence: 99%