3D Bioprinted GelMA‐Nanoclay Hydrogels Induce Colorectal Cancer Stem Cells Through Activating Wnt/β‐Catenin Signaling

Zhang, Yanmei; Wang, Zixuan; Hu, Qifan; Luo, Hao; Lu, Bingchuan; Gao, Yunhe; Qiao, Zhi; Zhou, Yongsen; Fang, Yajun; Gu, Jin; Zhang, Ting; Xiong, Zhuo

doi:10.1002/smll.202200364

Cited by 22 publications

(16 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 d,e). LGR5 and CD133 are well-known CSCs markers [ 20 ]. Then, we identified and sorted epithelial cells (EpCAM +) [ 21 ] and stem cells (EpCAM + /CD133 + , EpCAM + /LGR5 +) from the GC and GM populations (Figure S 1 a, b).…”

Section: Resultsmentioning

confidence: 99%

AQP5 complements LGR5 to determine the fates of gastric cancer stem cells through regulating ULK1 ubiquitination

Zhao

Bie

et al. 2022

J Exp Clin Cancer Res

View full text Add to dashboard Cite

Background Cancer stem cells (CSCs) are regarded as the "seed cells" for tumorigenesis, metastasis, recurrence and drug resistance. However, specific surface markers of CSCs of different origins have not been documented. Methods Single-cell sequencing was used to analyze the highly expressed genes in cancer stem cells of gastric cancer patients, and it was verified that AQP5 was specifically highly expressed in gastric cancer stem cells (GC-CSCs) in vivo and in vitro. The effect of AQP5-promoting LGR5 on the malignant biological function of GC-CSCs was investigated. The mechanism by which AQP5 affects GC-CSCs was explored through transcriptome sequencing, proteomic detection, mass spectrometry, etc. Results We report the identification and validation of AQP5 as a potentially specific surface marker of GC-CSCs. AQP5 was significantly upregulated in CSCs isolated from gastric cancer patients and in spheroid cells, and AQP5 was coexpressed with the canonical stem marker LGR5. Biologically, AQP5 promoted the sphere formation, proliferation, migration and invasion of GC cells in vitro and enhanced tumorigenesis in vivo. Furthermore, AQP5 coordinated with LGR5 and synergistically promoted the tumorigenesis of GC-CSCs. At the mechanistic level, AQP5 activated autophagy by inducing the LC3I/LC3II transformation in GC-CSCs, which was crucial for the biological functions of AQP5. Finally, we demonstrated that AQP5 recruited the E3 ligase TRIM21 to the key autophagy protein ULK1 and induced the K63-mediated ubiquitination of ULK1. Conclusions We elucidate a novel surface marker, AQP5, which is specifically expressed by GC-CSCs. Furthermore, our study creates a link between AQP5 and LGR5 and highlights the necessity of targeting both surface markers simultaneously as a promising approach for the treatment of gastric cancer patients.

show abstract

Section: Resultsmentioning

confidence: 99%

AQP5 complements LGR5 to determine the fates of gastric cancer stem cells through regulating ULK1 ubiquitination

Zhao

Bie

et al. 2022

J Exp Clin Cancer Res

View full text Add to dashboard Cite

show abstract

“…However, the relatively low mechanical properties of pure silk fibroin hydrogels limited the clinical applications. According to previous investigations, two-dimensional (2D) clay nanosheets could improve the hydrogel mechanical strength. − Therefore, 2D mica–Fe 3 O 4 (MF) nanosheets as an artificial peroxidase were synthesized and employed to prepare the silk fibroin nanozyme hydrogel. The method for nanozyme hydrogel preparation is illustrated in Figure a.…”

Section: Resultsmentioning

confidence: 99%

Silk Fibroin Nanozyme Hydrogel with Self-Supplied H₂O₂ for Enhanced Antibacterial Therapy

Li,

Yan,

et al. 2023

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

Peroxidase-mimicking nanozyme therapy has emerged as a powerful tool in antibacterial therapy. Due to the critical role in reactive oxygen species (ROS) generation, various in situ H 2 O 2 supplying nanoagents have been developed to guarantee the therapeutic effect. However, the challenges in reliance on external stimulus, instability of the H 2 O 2 donor, and risk of leakage limited their antibacterial efficiency and actual application. In this work, we fabricated an injectable silk fibroin/ ZnO NP/mica−Fe 3 O 4 (SFZM) composite nanozyme hydrogel with spontaneous H 2 O 2 generation and peroxidase-mimicking nanozyme properties. The ZnO NPs could produce H 2 O 2 without light or other external stimulus. Under a weak acid environment, the H 2 O 2 produced from ZnO NPs could be in situ transformed to ROS by magnetic mica−Fe 3 O 4 nanosheets, leading to nearly 50% enhancement in antibacterial activity compared with a silk fibroin−ZnO hydrogel. Meanwhile, the SFZM nanozyme hydrogel displayed in vivo adhesion and hemostasis properties. As a result, the SFZM hydrogel could accelerate the healing of bacteria-infected wounds in rats. More interestingly, the ZnO exhibited continued H 2 O 2 generation during at least 12 days, suggesting the long-term enhanced antibacterial effect of the SFZM hydrogel. Also, the SFZM nanozyme hydrogel showed low nanomaterial leakage and demonstrated biocompatibility both in vitro and in vivo.

show abstract

“…In addition, the mechanism governing stemness regulation was investigated, and GelMA-nanoclay hydrogel-activated Wnt/-catenin signaling was found to contribute to the induction and enrichment of CSCs. 78 To construct a TME, normal stromal cells were activated and reprogrammed into tumor-related stromal cells. Their results showed that tumor-related markers were overexpressed by the activated stromal cells, and they modified the ECM.…”

Section: Case Studies Of Bioprinting Cancermentioning

confidence: 99%

“…In conclusion, this model is appropriate for CRC tumor biology research studies and the creation of highly personalized cancer treatments. 2,77,78 Glioblastoma multiforme (GBM)…”

Section: Colorectal Cancer (Crc)mentioning

confidence: 99%

3D bioprinting complex models of cancer

Sharma

Perez²,

Silva

et al. 2023

Biomater. Sci.

View full text Add to dashboard Cite

show abstract

3D Bioprinted GelMA‐Nanoclay Hydrogels Induce Colorectal Cancer Stem Cells Through Activating Wnt/β‐Catenin Signaling

Abstract: two-tailed unpaired t-tests for comparisons of two groups. Significant differences between groups were noted as follows: * for p < 0.05, ** for p < 0.01, and n.s, not significant.

Cited by 22 publications

References 68 publications

AQP5 complements LGR5 to determine the fates of gastric cancer stem cells through regulating ULK1 ubiquitination

AQP5 complements LGR5 to determine the fates of gastric cancer stem cells through regulating ULK1 ubiquitination

Silk Fibroin Nanozyme Hydrogel with Self-Supplied H₂O₂ for Enhanced Antibacterial Therapy

3D bioprinting complex models of cancer

Contact Info

Product

Resources

About

3D Bioprinted GelMA‐Nanoclay Hydrogels Induce Colorectal Cancer Stem Cells Through Activating Wnt/β‐Catenin Signaling

Abstract: two-tailed unpaired t-tests for comparisons of two groups. Significant differences between groups were noted as follows: * for p < 0.05, ** for p < 0.01, and n.s, not significant.

Cited by 22 publications

References 68 publications

AQP5 complements LGR5 to determine the fates of gastric cancer stem cells through regulating ULK1 ubiquitination

AQP5 complements LGR5 to determine the fates of gastric cancer stem cells through regulating ULK1 ubiquitination

Silk Fibroin Nanozyme Hydrogel with Self-Supplied H2O2 for Enhanced Antibacterial Therapy

3D bioprinting complex models of cancer

Contact Info

Product

Resources

About

Silk Fibroin Nanozyme Hydrogel with Self-Supplied H₂O₂ for Enhanced Antibacterial Therapy